Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
1.0K Views
•
07:48 min
May 5th, 2023
DOI :
10.3791/65158-v
Chapters
0:04
Introduction
0:46
Self Healing Annealable Particle: Extracellular Matrix (SHAPE) Composite Preparation
2:40
Human Neural Stem Cell (hNSC) Bioink Preparation and Embedded 3D Printing
6:19
Results: Characterization of 3D Printed Human Neural Construct
7:11
Conclusion
Transcript
SHAPE复合材料中的嵌入式3D打印克服了传统的生物制造限制,能够对类似于天然细胞外环境的水凝胶内的机械敏感组织进行准确的图案化。SHAPE工具箱依赖于模块化生物材料设计,可以轻松更改打印载体配方。它还利用低成本材料和可访问的设备,以便其他研究小组进行简单的调整。
这种方法可用于创建空间定义的人类神经组织模型,以检查帕金森氏症等神经退行性疾病的神经元发育和通信。首先在超纯水中以每毫升2毫克的浓度制备碳酸钙溶液。将其与海藻酸盐溶液以相等的比例混合,并在室温下以650RPM磁力搅拌
Sign in or start your free trial to access this content
Summary
这项工作描述了在自修复可退火颗粒 - 细胞外基质复合材料中自由形式嵌入神经干细胞的协议。该协议能够以高保真度对互连的人类神经组织结构进行可编程模式化。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved