We explore entomopathogenic fungal resources from wild Monochamus alternatus populations. Taking this species as an example, we could enrich the diversity of entomopathogenic fungi of other forest wood borers for future bio-control applications. We have recently obtained three fungal species certified as parasitical entomopathogenic fungi of Monochamus alternatus, two of which are not well-known, but have proven to be potent pathogens of insects.
This protocol helps the discovery of more EPFS of Monochamus alternatus, and performs a substitute way to evaluate their entomopathogenic activities, which can also provide insights into entomopathogenic fungal exploration for other forest wood borers.