Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency

658 Views

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07:08 min

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February 2nd, 2024

DOI :

10.3791/65811-v

February 2nd, 2024

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Amani Alshaikh¹^,², Dmytro Grygoryev³, Dove Keith⁴, Brett Sheppard⁴^,⁵, Rosalie C Sears⁴^,⁶, Jungsun Kim³^,⁶, Abdenour Soufi¹

¹Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research, The University of Edinburgh, ²King Abdulaziz City for Science and Technology Health Sector (KACST), ³Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, ⁴Brenden-Colson Canter for Pancreatic Care, Oregon Health & Science University, ⁵Department of Surgery, Oregon Health & Science University, ⁶Department of Molecular & Medical Genetics, Oregon Health & Science University

Summary

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Pancreatic Ductal Adenocarcinoma

Reprogramming Protocol

Chapters in this video

0:00

Introduction

1:29

Lentivirus Preparation and Reprogramming Transduction for Pancreatic Ductal Adenocarcinoma Cells

4:22

Preparation of iMEF Feeder Cells and Reprogramming Transduction for Induced Pluripotent Stem Cells

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