The Colorado potato beetle is one of the most serious pests of potato in temperate zone. Studies of the immunity and pathogenesis of this pest are mainly carried out at the active stage. However, in temperate zone, this pest spend most of its life
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Here we present a method for studying Colorado potato beetle hibernation under the natural conditions of the temperate zone as well as a technique for collecting beetles in winter. This method allows to obtain a desired number of overwintering individuals for various analyses at any stage of hibernation.