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	<tbody>
		<tr>
			<td>A83-01- ALK5 Inhibitor IV</td>
			<td>Twin Helix</td>
			<td>&#160;T3031</td>
			<td></td>
		</tr>
		<tr>
			<td>B27</td>
			<td>Thermofisher Scientific</td>
			<td>0080085SA</td>
			<td></td>
		</tr>
		<tr>
			<td>CFX Connect Real-Time PCR Detection System&#160;</td>
			<td>&#160;Bio-Rad</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>CHIR99021</td>
			<td>Twin Helix</td>
			<td>T2310</td>
			<td></td>
		</tr>
		<tr>
			<td>Click EdU Alexa 488 imaging kit</td>
			<td>Thermofisher Scientific</td>
			<td>C10499</td>
			<td></td>
		</tr>
		<tr>
			<td>Collagen, Type I, solution from rat tail</td>
			<td>Merck</td>
			<td>C3867-1VL</td>
			<td></td>
		</tr>
		<tr>
			<td>Dexamethasone&#160;</td>
			<td>Merck</td>
			<td>D4902</td>
			<td></td>
		</tr>
		<tr>
			<td>EGF</td>
			<td>Merck</td>
			<td>&#160;E9644</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal bovine serum (FBS)</td>
			<td>Euroclone</td>
			<td>ECS0180L</td>
			<td></td>
		</tr>
		<tr>
			<td>GELTREX LDEV FREE RGF BME</td>
			<td>Thermofisher Scientific</td>
			<td>A1413202</td>
			<td></td>
		</tr>
		<tr>
			<td>Heparin Sodium 25000 IU/5 ml</td>
			<td>B. Braun Melsungen AG</td>
			<td>B01AB01</td>
			<td></td>
		</tr>
		<tr>
			<td>HGF</td>
			<td>Peprotech</td>
			<td>&#160;100-39H&#160;</td>
			<td></td>
		</tr>
		<tr>
			<td>Insulin-Transferrin-Selenium solution 100x&#160;</td>
			<td>Thermofisher Scientific</td>
			<td>41400045</td>
			<td></td>
		</tr>
		<tr>
			<td>L-Glutamine solution</td>
			<td>Euroclone</td>
			<td>ECB3000D</td>
			<td></td>
		</tr>
		<tr>
			<td>Liver Digest Medium&#160;</td>
			<td>Thermofisher Scientific</td>
			<td>&#160;17703-034</td>
			<td></td>
		</tr>
		<tr>
			<td>Liver Perfusion Medium</td>
			<td>Thermofisher Scientific</td>
			<td>17701038</td>
			<td></td>
		</tr>
		<tr>
			<td>N2 supplement</td>
			<td>Thermofisher Scientific</td>
			<td>17502048</td>
			<td></td>
		</tr>
		<tr>
			<td>N-acetylcysteine</td>
			<td>Merck</td>
			<td>A9165</td>
			<td></td>
		</tr>
		<tr>
			<td>Nicotinamide</td>
			<td>Merck</td>
			<td>&#160;N-0636</td>
			<td></td>
		</tr>
		<tr>
			<td>Non-Essential Amino Acids</td>
			<td>Merck</td>
			<td>M7145</td>
			<td></td>
		</tr>
		<tr>
			<td>Normocin</td>
			<td>Aurogene</td>
			<td>ant-nr-1</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS buffer 1X</td>
			<td>PanReac AppliChem</td>
			<td>A0964,9050</td>
			<td></td>
		</tr>
		<tr>
			<td>Penicillin-streptomycin solution 100x</td>
			<td>Euroclone</td>
			<td>ECB3001D</td>
			<td></td>
		</tr>
		<tr>
			<td>Percoll</td>
			<td>Santa Cruz</td>
			<td>sc-296039A</td>
			<td></td>
		</tr>
		<tr>
			<td>Peristaltic pump</td>
			<td>Ismatec&#8482;&#160;</td>
			<td>MS-4/12 Reglo Digital Pump</td>
			<td></td>
		</tr>
		<tr>
			<td>TNFa</td>
			<td>Peprotech</td>
			<td>&#160;300-01A</td>
			<td></td>
		</tr>
		<tr>
			<td>TRI Reagent</td>
			<td>Merck</td>
			<td>T9424</td>
			<td></td>
		</tr>
		<tr>
			<td>Tubing&#160;</td>
			<td>Ismatec&#8482;&#160;</td>
			<td>&#160;ID.2,79mm</td>
			<td></td>
		</tr>
		<tr>
			<td>Williams&#39; E Medium, no glutamine</td>
			<td>Thermofisher Scientific</td>
			<td>31415029</td>
			<td></td>
		</tr>
		<tr>
			<td>Y27632</td>
			<td>Twin Helix</td>
			<td>T1725</td>
			<td></td>
		</tr>
	</tbody>
</table>

rat liver perfusion and primary hepatocytes isolation: an old procedure crucial for cutting-edge 3d organoids culture

大鼠肝脏灌注和原代肝细胞分离：一种对尖端 3D 类器官培养至关重要的旧程序

Primary hepatocytes are a commonly used tool for in vitro liver-related studies. However, the maintenance of these cells has always been a challenge due ...

rat-liver-perfusion-primary-hepatocytes-isolation-an-old-procedure

Research

JoVE Journal

Biology

Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture

423 Views.  Italian Liver Foundation. Introduction.Primary hepatocytes are an important tool for in vitro liver related studies. However, the expansion and maintenance of these cells have been historically challenging as they lose morphology and functionality after a few days in culture. 3D organoids are a cutting-edge tool able to recapitulate tissues in a dish for a long-term culture, overcoming the problem of the inability to expand to the primary hepatocytes in vitro.The overall goal of the present work is to describe ...