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13:09 min
November 22nd, 2024
DOI :
10.3791/66857-v
Chapters
0:23
Introduction
1:19
Preparation for the Animal Procedure
2:07
Preparation for Hepatocytes Isolation
2:16
Initial Animal Procedure & Anesthesia
3:48
Cannulation & Liver Perfusion
5:14
Liver Digestion
6:35
Hepatocyte Purification
8:50
Hepatocyte Culture & RNA Collection
10:10
Representative Results
12:06
Conclusion
Transcript
原代肝细胞是体外肝脏相关研究的重要工具。然而,这些细胞的扩增和维持历来具有挑战性,因为它们在培养几天后会失去形态和功能。3D 类器官是一种尖端工具,能够在培养皿中重现组织以进行长期培养,克服了在体外无法扩增至原代肝细胞的问题。
这项工作的总体目标是详尽、详细和重要的方案,描述大鼠肝脏灌注和原代肝细胞分离的关键步骤,以使研究人员能够加速和优化 3D 类器官培养,在 42 摄氏度下进行温水浴,然后将灌注缓冲液和 P
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Summary
该方案在大鼠模型中提出了一种优化的两步胶原酶肝灌注技术,并展示了使用分离的肝细胞进行 3D 类器官的 体外 长期培养。
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大鼠肝脏灌注和原代肝细胞分离:一种对尖端 3D 类器官培养至关重要的旧程序
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