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Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture

687 Views

13:09 min

November 22nd, 2024

DOI :

10.3791/66857-v

November 22nd, 2024


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Biology

Chapters in this video

0:23

Introduction

1:19

Preparation for the Animal Procedure

2:07

Preparation for Hepatocytes Isolation

2:16

Initial Animal Procedure & Anesthesia

3:48

Cannulation & Liver Perfusion

5:14

Liver Digestion

6:35

Hepatocyte Purification

8:50

Hepatocyte Culture & RNA Collection

10:10

Representative Results

12:06

Conclusion

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