Name: Pleins feux sur l’auteur : Extraction écologique de composés bioactifs à l’aide de techniques assistées par micro-ondes à base de polyols
Uploaded: 2024-08-23T13:40:00
Description: The utilization of polyols as green solvents for extracting bioactive compounds from plant materials has gained attention due to their safety and inert ...

Cette étude a été financée par l’Université Mae Fah Luang. Les auteurs tiennent à remercier l’Institut du thé et du café de l’Université de Mae Fah Luang d’avoir facilité la connexion entre les chercheurs et les agriculteurs locaux concernant l’acquisition d’échantillons de café argenté.

Les détails des réactifs et de l’équipement utilisés dans cette étude sont énumérés dans la table des matériaux.
1. Préparation expérimentale
<ol><li>Préparation d’échantillons de plantes<ol><li>Récupérez le café frais silverskin (Coffea arabica) et séchez-le à 60 °C dans un séchoir à plateaux pendant 72 h11.</li>
		<li>Broyez le café séché en une poudre fine à l’aide d’un moulin et conservez-...

Extraction assistée par micro-ondes à base de polyol pour des rendements phénoliques et antioxydants

<ol>
	<li>Wawoczny, A., Gillner, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+most+potent+natural+pharmaceuticals,+cosmetics,+and+food+ingredients+isolated+from+plants+with+deep+eutectic+solvents.">The most potent natural pharmaceuticals, cosmetics, and food ingredients isolated from plants with deep eutectic solvents.</a> J Agric Food Chem. 71 (29), 10877-10900 (2023).</li><li>Syukur, M., Prahasiwi, M. 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O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sustainability+of+solvent+extraction+techniques+in+pollution+prevention+and+control.">Sustainability of solvent extraction techniques in pollution prevention and control.</a> Handbook of advanced approaches towards pollution prevention and control. , 33-66 (2021).</li><li>P&#322;otka-Wasylka, J., Rutkowska, M., Owczarek, K., Tobiszewski, M., Namie&#347;nik, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extraction+with+environmentally+friendly+solvents.">Extraction with environmentally friendly solvents.</a> TrAC, Trends Anal Chem. 91, 12-25 (2017).</li><li>Queffelec, J., Beraud, W., Torres, M. D., Dom&#237;nguez, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Advances+in+obtaining+ready-to-use+extracts+with+natural+solvents.">Advances in obtaining ready-to-use extracts with natural solvents.</a> Sustain Chem Pharm. 38, 101478 (2024).</li><li>Can Karaca, A., Erdem, I. G., Ak, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+polyols+on+gelation+kinetics,+gel+hardness,+and+drying+properties+of+alginates+subjected+to+internal+gelation.">Effects of polyols on gelation kinetics, gel hardness, and drying properties of alginates subjected to internal gelation.</a> LWT. 92, 297-303 (2018).</li><li>Nastasi, J. R., Daygon, V. D., Kontogiorgos, V., Fitzgerald, M. 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Divers facteurs jouent un rôle crucial dans la mise en œuvre réussie de l’EDM, tels que le contenu phytochimique des composants végétaux, la durée d’extraction, la température, la puissance des micro-ondes, le rapport solide-liquide et la concentration en solvant13. Les plantes présentent généralement des profils variables de composés phytochimiques ; Par conséquent, la sélection de plantes naturelles riches en antioxydants et en composés phénoliques est essentielle<sup class="...

De nos jours, il existe une tendance mondiale à la prise de conscience environnementale dans l’industrie de la beauté, ce qui conduit les fabricants à se concentrer sur les technologies vertes pour extraire les composants végétaux à l’aide d’alternatives durables1. En règle générale, les solvants traditionnels tels que l’éthanol, le méthanol et l’hexane sont utilisés pour extraire les composants phénoliques végétaux et les antioxydants naturels2. Néanmoins, la présence de résidus de solvants dans les extraits de plantes présente un risque potentiel pour la santé humaine, induisant une irritation cutanée et oculaire3, notamment en ce qui concerne leur utilisation prévue en cosmétique. Par conséquent, il est difficile d’éliminer ces résidus de solvants des extraits, un processus qui nécessite un investissement considérable en temps, en énergie et en ressources humaines4. Récemment, l’eau surchauffée, les liquides ioniques, les solvants eutectiques profonds et les solvants biodérivés sont apparus comme des approches prometteuses pour l’extraction par solvant vert5. Cependant, leur utilisation est encore limitée par la séparation des produits dans les procédés aqueux. Pour répondre à ces enjeux, le développement d’extraits prêts à l’emploi apparaît comme une solution viable6.
Les polyols sont souvent utilisés dans les formulations cosmétiques en tant qu’humectants en raison de leur bonne polarité et de leur capacité à retenir l’humidité de l’environnement7. De plus, des polyols tels que la glycérine, le propylène glycol, le butylène glycol, le méthylpropanediol, l’isopentyldiol, le pentylène glycol, le 1,2-hexanediol et l’hexylène glycol peuvent être utilisés pour l’extraction de plantes. Ils sont considérés comme des solvants non toxiques, biodégradables, respectueux de l’environnement, non réactifs et sûrs pour l’extraction des plantes8. De plus, les polyols peuvent résister à la chaleur générée lors de l’extraction assistée par micro-ondes (MAE) en raison de leur point d’ébullition élevé et de leur polarité9. Ces polyols sont généralement reconnus comme des produits chimiques sûrs (GRAS) par la Food and Drug Administration (FDA) des États-Unis. Contrairement aux solvants conventionnels tels que l’éthanol ou le méthanol, qui peuvent nécessiter une élimination rigoureuse de l’extrait en raison de leurs effets potentiellement nocifs, les polyols offrent l’avantage de minimiser l’énergie, le temps et les coûts associés aux processus d’élimination des solvants10. Cela permet non seulement de rationaliser le processus d’extraction, mais aussi d’améliorer l’efficacité et la durabilité globales de la méthode d’extraction. Des recherches antérieures ont utilisé des polyols tels que le propylène glycol et le butylène glycol comme solvants dans l’extraction de composés bioactifs des fleurs de Camellia sinensis 10 et de la pulpe de café11, révélant un potentiel important pour leur rôle en tant que solvants alternatifs durables dans le processus d’extraction des plantes. Ainsi, le développement et l’optimisation continus d’un système de solvants polyols-eau offrent un potentiel de progrès significatifs en chimie verte et en pratiques industrielles durables.
En général, les composés bioactifs présents dans les plantes sont synthétisés sous forme de métabolites secondaires. Ces composés peuvent être classés en trois groupes principaux : les terpènes et les terpénoïdes, les alcaloïdes et les composés phénoliques12. Diverses méthodes d’extraction sont utilisées dans différentes conditions pour isoler des composés bioactifs spécifiques des plantes. Les composés bioactifs des matières végétales peuvent être extraits à l’aide de techniques conventionnelles ou non conventionnelles. Les méthodes traditionnelles comprennent la macération, l’extraction par reflux et l’hydrodistillation, tandis que les méthodes non conventionnelles comprennent l’extraction assistée par ultrasons, l’extraction assistée par enzymes, l’extraction assistée par micro-ondes (MAE), l’extraction assistée par champ électrique pulsé, l’extraction par fluide supercritique et l’extraction par liquide sous pression13. Ces méthodes non conventionnelles sont conçues pour améliorer la sécurité en utilisant des solvants et des auxiliaires plus sûrs, en améliorant l’efficacité énergétique, en empêchant la dégradation des composants bioactifs et en réduisant la pollution de l’environnement14.
De plus, le MAE fait partie des technologies vertes sophistiquées pour extraire les composés bioactifs des plantes. Les procédures d’extraction conventionnelles nécessitent beaucoup de temps, d’énergie et des températures élevées, ce qui, au fil du temps, peut dégrader les composés bioactifs sensibles à la chaleur13. Contrairement aux extractions thermiques conventionnelles, la MAE facilite l’extraction de composés bioactifs en générant un chauffage localisé dans l’échantillon, en perturbant les structures cellulaires et en améliorant le transfert de masse, augmentant ainsi l’efficacité de l’extraction des composés. La chaleur est transférée de l’intérieur des cellules végétales par des micro-ondes, qui agissent sur les molécules d’eau à l’intérieur des composants de la plante13. De plus, le MAE a progressé pour améliorer l’extraction et la séparation des composés actifs, augmenter le rendement du produit, améliorer l’efficacité de l’extraction, nécessiter moins de produits chimiques et économiser du temps et de l’énergie tout en empêchant la destruction des composés bioactifs15.
Cette recherche porte sur l’extraction de composés phénoliques végétaux et d’antioxydants naturels par extraction assistée par micro-ondes (MAE) en utilisant différents types de polyols comme solvants. La teneur totale en phénols (TPC), la teneur totale en flavonoïdes (TFC) et les activités antioxydantes (DPPH, ABTS et FRAP) des extraits MAE à base de polyols sont déterminées. De plus, l’EAM à base de polyols est comparé à l’utilisation de solvants conventionnels tels que l’eau et l’éthanol. Cette recherche devrait contribuer au développement d’une technologie d’extraction écologiquement durable pour les composants naturels, en promouvant la durabilité en réduisant la dépendance aux produits chimiques dangereux, en raccourcissant les temps de traitement et en minimisant la consommation d’énergie dans la production de matières premières pour des applications potentielles dans l’industrie cosmétique.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1,2-Hexanediol</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>2,2 -Azino-bis 3 ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS)</td>
			<td>Sigma</td>
			<td>A1888</td>
			<td></td>
		</tr>
		<tr>
			<td>2,2-Diphenyl-1-picrylhydrazyl (DPPH)</td>
			<td>Sigma</td>
			<td>D9132</td>
			<td></td>
		</tr>
		<tr>
			<td>2,4,6-Tri(2-pyridyl)-s-triazine (TPTZ)</td>
			<td>Sigma</td>
			<td>93285</td>
			<td></td>
		</tr>
		<tr>
			<td>2-Digital balance</td>
			<td>Ohaus</td>
			<td></td>
			<td>Pioneer</td>
		</tr>
		<tr>
			<td>4-Digital balance</td>
			<td>Denver</td>
			<td>SI-234</td>
			<td></td>
		</tr>
		<tr>
			<td>6-hydroxy-2,5,7,8 tetramethylchroman -2-carboxylic acid (Trolox)</td>
			<td>Sigma</td>
			<td>238813</td>
			<td></td>
		</tr>
		<tr>
			<td>96-well plate</td>
			<td>SPL Life Science</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Absolute ethanol</td>
			<td>RCI Labscan</td>
			<td>64175</td>
			<td></td>
		</tr>
		<tr>
			<td>Acetic acid</td>
			<td>RCI Labscan</td>
			<td>64197</td>
			<td></td>
		</tr>
		<tr>
			<td>Aluminum chloride</td>
			<td>Loba Chemie</td>
			<td>898</td>
			<td></td>
		</tr>
		<tr>
			<td>Automatic pipette</td>
			<td>Labnet</td>
			<td></td>
			<td>Biopett</td>
		</tr>
		<tr>
			<td>Butylene glycol</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Ethos X advanced microwave extraction</td>
			<td>Milestone Srl, Sorisole, Italy</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Ferrous sulfate</td>
			<td>Ajex Finechem</td>
			<td>3850</td>
			<td></td>
		</tr>
		<tr>
			<td>Folin-Ciocalteu&#39;s reagent</td>
			<td>Loba Chemie</td>
			<td>3870</td>
			<td></td>
		</tr>
		<tr>
			<td>Freezer SF</td>
			<td>Sanyo</td>
			<td>C697(GYN)</td>
			<td></td>
		</tr>
		<tr>
			<td>Gallic acid</td>
			<td>Sigma</td>
			<td>398225</td>
			<td></td>
		</tr>
		<tr>
			<td>Grinder</td>
			<td>Ou Hardware Products Co.,Ltd</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Hexylene glycol</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrochloric acid (37%)</td>
			<td>RCI Labscan</td>
			<td>AR1107</td>
			<td></td>
		</tr>
		<tr>
			<td>Iron (III) chloride</td>
			<td>Loba Chemie</td>
			<td>3820</td>
			<td></td>
		</tr>
		<tr>
			<td>Isopentyldiol</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Methanol</td>
			<td>RCI Labscan</td>
			<td>67561</td>
			<td></td>
		</tr>
		<tr>
			<td>Methylpropanediol&#160;</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Pentylene glycol</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Potassium persulfate</td>
			<td>Loba Chemie</td>
			<td>5420</td>
			<td></td>
		</tr>
		<tr>
			<td>Propylene glycol</td>
			<td>Chanjao Longevity Co., Ltd.</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Quercetin</td>
			<td>Sigma</td>
			<td>Q4951</td>
			<td></td>
		</tr>
		<tr>
			<td>Refrigerated centrifuge</td>
			<td>Hettich</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium acetate</td>
			<td>Loba Chemie</td>
			<td>5758</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium carbonate</td>
			<td>Loba Chemie</td>
			<td>5810</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium hydroxide</td>
			<td>RCI Labscan</td>
			<td>AR1325</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium nitrite</td>
			<td>Loba Chemie</td>
			<td>5954</td>
			<td></td>
		</tr>
		<tr>
			<td>SPECTROstar Nano microplate reader</td>
			<td>BMG- LABTECH</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>SPSS software</td>
			<td>IBM SPSS Statistics 20</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Tray dryer</td>
			<td>France Etuves</td>
			<td>XUE343</td>
			<td></td>
		</tr>
	</tbody>
</table>

microwave-assisted extraction of phenolic compounds and antioxidants for cosmetic applications using polyol-based technology

L’utilisation de polyols comme solvants verts pour l’extraction de composés bioactifs à partir de matières végétales a attiré l’attention en raison de leur sécurité et de leur comportement inerte avec les produits chimiques bioactifs végétaux. Cette étude explore l’extraction durable de composés phénoliques et d’antioxydants naturels à partir de la peau d’argent du café à l’aide de la méthode d’extraction assistée par micro-ondes (MAE) avec des solvants à base de polyols : glycérine, propylène glycol (PG), butylène glycol (BG), méthylpropanediol (MPD), isopentyldiol (IPD), pentylène glycol, 1,2-hexanediol et hexylène glycol (HG). Une analyse comparative a été effectuée sur les extractions par solvant conventionnelles et non conventionnelles, en mettant l’accent sur leur impact sur les composés bioactifs de l’EAM, englobant des paramètres tels que la teneur phénolique totale (TPC), la teneur totale en flavonoïdes (TFC) et les activités antioxydantes telles que le test de piégeage radical 1,1-diphényl-2-picrylhydrazyle (DPPH), le test de piégeage radical de l’acide 2,2-azino-bis(-3-éthylbenzothiazoline-6-sulfonique) (ABTS) et le test de piégeage antioxydant réducteur ferrique (FRAP). Les valeurs les plus élevées ont été observées pour le TPC avec l’extraction aqueuse au 1,2-hexanediol (échantillon de 52,0 ± 3,0 mg de GAE/g), le TFC avec l’extraction aqueuse au 1,2-hexanediol (échantillon de 20,0 ± 1,7 mg QE/g), le DPPH avec l’extraction aqueuse au HG (échantillon de 13,6 ± 0,3 mg TE/g), l’ABTS avec l’extraction aqueuse au pentylène glycol (échantillon de 8,2 ± 0,1 mg TE/g) et le FRAP avec l’extraction aqueuse au HG (échantillon de 21,1 ± 1,3 mg Fe (II)). Cette recherche vise à faire progresser la technologie d’extraction respectueuse de l’environnement grâce à des composants végétaux naturels, en favorisant la durabilité en minimisant l’utilisation de produits chimiques dangereux tout en réduisant la consommation de temps et d’énergie, avec des applications potentielles dans les cosmétiques.

Nowadays, there is a global trend towards environmental awareness in the beauty industry, leading manufacturers to focus on green technology for extracting plant components using sustainable alternatives1. Typically, traditional solvents such as ethanol, methanol, and hexane are used to extract plant phenolic components and natural antioxidants2. Nevertheless, the presence of solvent residues within plant extracts poses a potential risk to human health, inducing skin and eye irritation3, particularly concerning their intended application in cosmetics. Consequently, it is challenging to eliminate such solvent residues from the extracts, a process that demands considerable investment in time, energy, and human resources4. Recently, superheated water, ionic liquids, deep eutectic solvents, and bio-derived solvents have emerged as promising approaches for green solvent extraction5. However, their use is still limited by product separation in aqueous-based processes. To address these challenges, the development of ready-to-use extracts emerges as a viable solution6.
Polyols are often used in cosmetic formulations as humectants because of their good polarity and ability to retain moisture from the environment7. In addition, polyols such as glycerin, propylene glycol, butylene glycol, methylpropanediol, isopentyldiol, pentylene glycol, 1,2-hexanediol, and hexylene glycol can be utilized for plant extractions. They are considered non-toxic, biodegradable, environmentally friendly, non-reactive, and safe solvents for use in plant extraction8. Additionally, polyols can withstand the heat generated during microwave-assisted extraction (MAE) due to their elevated boiling points and polarity9. These polyols are generally recognized as safe (GRAS) chemicals by the United States Food and Drug Administration (FDA). Unlike conventional solvents such as ethanol or methanol, which may require rigorous removal from the extract due to their potentially harmful effects, polyols offer the advantage of minimizing the energy, time, and costs associated with solvent removal processes10. This not only streamlines the extraction process but also enhances the overall efficiency and sustainability of the extraction method. Previous investigations have employed polyols such as propylene glycol and butylene glycol as solvents in the extraction of bioactive compounds from Camellia sinensis flowers10 and coffee pulp11, revealing significant potential for their role as sustainable alternative solvents in the plant extraction process. Thus, the continued development and optimization of a polyols-water solvent system holds the potential for significant advancements in green chemistry and sustainable industrial practices.
Generally, bioactive compounds found in plants are synthesized as secondary metabolites. These compounds can be categorized into three primary groups: terpenes and terpenoids, alkaloids, and phenolic compounds12. Various extraction methods are utilized under different conditions to isolate specific bioactive compounds from plants. Bioactive compounds from plant materials can be extracted using either conventional or non-conventional techniques. Traditional methods include maceration, reflux extraction, and hydro-distillation, while non-conventional methods consist of ultrasound-assisted extraction, enzyme-assisted extraction, microwave-assisted extraction (MAE), pulsed electric field-assisted extraction, supercritical fluid extraction, and pressurized liquid extraction13. These non-conventional methods are designed to enhance safety by utilizing safer solvents and auxiliaries, improving energy efficiency, preventing degradation of the bioactive components, and reducing environmental pollution14.
Furthermore, MAE is among the sophisticated green technologies for extracting bioactive compounds from plants. Conventional extraction procedures require significant amounts of time, energy, and high temperatures, which over time might degrade heat-sensitive bioactive compounds13. In contrast to conventional thermal extractions, MAE facilitates the extraction of bioactive compounds by generating localized heating within the sample, disrupting cell structures, and enhancing mass transfer, thereby increasing the efficiency of compound extraction. Heat is transferred from inside the plant cells by microwaves, which operate on the water molecules within the plant components13. Moreover, MAE has advanced to improve the extraction and separation of active compounds, increasing product yield, enhancing extraction efficiency, requiring fewer chemicals, and saving time and energy while preventing the destruction of bioactive compounds15.
This research focuses on the extraction of plant phenolic compounds and natural antioxidants through microwave-assisted extraction (MAE) using different types of polyols as solvents. The total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities (DPPH, ABTS, and FRAP) of polyol-based MAE extracts are determined. Additionally, polyol-based MAE is compared with MAE using conventional solvents such as water and ethanol. This research is expected to contribute to the development of environmentally sustainable extraction technology for natural components, promoting sustainability by reducing reliance on hazardous chemicals, shortening processing times, and minimizing energy consumption in raw material production for potential applications within the cosmetics industry.

Pleins feux sur l’auteur : Extraction écologique de composés bioactifs à l’aide de techniques assistées par micro-ondes à base de polyols

Extraction assistée par micro-ondes de composés phénoliques et d’antioxydants pour des applications cosmétiques à l’aide de la technologie à base de polyols

This research talks about finding eco-friendly ways to extract plant bioactive compounds. We are testing how well microwave assisted extraction with the polyol-based solvents work for extracting plants bioactive substances, especially phenol compounds. Additionally, exploring this method could lead to more efficient and effective extraction technique for the cosmetic industries.Today's cosmetic research is also about using green technology and green chemistry. Nonconventional extraction techniques such as microwave-assist extraction, and extraction, have been interestingly employed to extract plants bioactive compounds. However, organic solvents are still commonly utilized for bioactive component extraction.Using polyol-based solvent for microwave assistant extraction turned out to be more effective than using traditional solvent like water and ethanol. The results show that the plant extract obtained with polyol have higher level of phenolic content for the flavanoid content and antioxidant activity. Compared to those extracted with conventional solvent.Our procedure stands out because it is beneficial, environmentally friendly and safe for human health. It is also more energy efficient and cost effective than traditional processes, making it an excellent alternative for sustainable and simple production. Our finding show that polyols combined with unconventional extraction methods can extract phenol compounds.The next challenge is whether this extraction method can be used to extract other plant, secondary metabolized, and if it can be commercialized to extract bioactive compounds for cosmetic purposes.

Effet des solvants polyols et des solvants conventionnels sur la teneur totale en phénols, la teneur totale en flavonoïdes, les dosages antioxydants DPPH, FRAP et ABTS La polarité du solvant doit être compatible avec celle des molécules actives ciblées afin d’améliorer l’efficacité d’extraction des substances bioactives des plantes22. Des expériences ont été menées à l’aide de divers solvants (eau, éthanol, glycérine, propylène glycol, butylène glycol, ...

Ce protocole détaille l’utilisation d’une méthode d’extraction assistée par micro-ondes à base de polyols pour extraire les composés phénoliques et les antioxydants naturels, représentant une approche pratique et écologiquement durable pour le développement d’extraits prêts à l’emploi.

The utilization of polyols as green solvents for extracting bioactive compounds from plant materials has gained attention due to their safety and inert ...

microwave-assisted-extraction-phenolic-compounds-antioxidants-for

Research

JoVE Journal

Chemistry

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

The utilization of polyols as green solvents for extracting bioactive compounds from plant materials has gained attention due to their safety and inert behavior with plant bioactive chemicals. This study explores the sustainable extraction of phenolic compounds and natural antioxidants from coffee silverskin using the microwave-assisted extraction (MAE) method with polyol-based solvents: glycerin, propylene glycol (PG), butylene glycol (BG), methylpropanediol (MPD), isopentyldiol (IPD), pentylene glycol, 1,2-hexanediol, and hexylene glycol (HG). A comparative analysis was conducted on conventional and non-conventional solvent extractions, focusing on their impact on the bioactive compounds of MAE, encompassing parameters such as total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities like the 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH), the 2,2&#8242;-azino-bis(-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assay (ABTS), and the ferric reducing antioxidant power assay (FRAP). The highest values were observed for TPC with aqueous-1,2-hexanediol extraction (52.0 &#177; 3.0 mg GAE/g sample), TFC with aqueous-1,2-hexanediol extraction (20.0 &#177; 1.7 mg QE/g sample), DPPH with aqueous-HG&#160;extraction (13.6 &#177; 0.3 mg TE/g sample), ABTS with aqueous-pentylene glycol extraction (8.2 &#177; 0.1 mg TE/g sample), and FRAP with aqueous-HG extraction (21.1 &#177; 1.3 mg Fe (II) E/g sample). This research aims to advance eco-friendly extraction technology through natural plant components, promoting sustainability by minimizing hazardous chemical use while reducing time and energy consumption, with potential applications in cosmetics.

This protocol details the utilization of a polyol-based microwave-assisted extraction method for extracting phenolic compounds and natural antioxidants, representing a practical and environmentally sustainable approach to the development of ready-to-use extracts.

Recherche

Chimie

Extraction of Phenolic Compounds and Natural Antioxidants from Coffee Silverskin Using the Polyol-Based Microwave-Assisted Extraction Method

To begin, place the diluted 60%solvents for microwave assisted extraction on the working platform. Weigh 0.67 grams of the coffee silver skin and mix with 20 milliliters of extraction solvent at a one is to 30 ratio in a reaction container. Add a magnetic stir bar to the vessel to ensure uniform distribution of the heat and solvent within the sample.Close the vessel firmly with a special tool and place it into the microwave assisted extraction chamber. To set up the method, click on the toolbox icon on the top bar of the monitor and select the SK eT rotor in the accessory section. Then click on the stir and type 20%to set the stirring rate.Click on the door lock sector and set it to activate at temperatures exceeding 80 degrees Celsius. Then click on the table icon on the top bar and set the temperature gradient T1 to an extraction duration of 10 minutes. Microwave power to 1800 watts and temperature to 120 degrees Celsius.To activate the stir, click on the stir button and wait until the green light appears. Set the blower fan speed to level three. To hold the extraction time, select the desired extraction temperature T2 by setting the extraction duration to 15 minutes, the microwave power to 1800 watts and the temperature to 120 degrees Celsius.Now, click on the cooling button at the lower left corner of the screen and select the cooling time of 10 minutes. Click the Save icon at the top right corner of the screen. Start the extraction process by choosing the number of vessels used.Following extraction, centrifuge extracts at 9072g for 15 minutes at four degrees Celsius. Collect the supernatant with a 10 milliliter glass pipette and store it at minus 20 degrees Celsius. Dilute the coffee silver skin extracts tenfold with distilled water in a 96 well plate.Mix 10 microliters of the diluted sample with 20 microliters of undiluted folin-ciocalteu reagent, and allow them to react for three minutes. Next, add 100 microliters of 7.5%sodium carbonate solution to the mixture in each well of a 96 well plate. Prepare different concentrations for the gallic acid standard concentration range by diluting with distilled water.Mix them with 20 microliters of folin-ciocalteu reagent, and allow them to react for three minutes. Next, add 100 microliters of 7.5%sodium carbonate solution to the mixture in each well of a 96 well plate. Incubate the reaction for 30 minutes in the dark at room temperature.On a microplate reader, measure the absorbance of the reaction mixture at 765 nanometers. Plot the standard calibration curve using the concentrations of the standard and absorbance at 765 nanometers. Express the results as milligrams of gallic acid equivalent per gram of the sample.Aqueous 1, 2 hexanediol extraction yielded the highest total phenolic content and water extraction yielded the lowest. Aqueous 1, 2 hexanediol extraction yielded the highest total flavonoid content and aqueous isopentyldiol extraction yielded the lowest. Aqueous hexylene glycol extraction exhibited the highest DPPH assay value and aqueous ethanol extraction exhibited the lowest.Aqueous pentylene glycol extraction resulted in the highest ABTS assay value and water extraction resulted in the lowest. Aqueous hexylene glycol extraction showed the highest FRAP value and water extraction showed the lowest.

Extraction de composés phénoliques et d’antioxydants naturels à partir de peau d’argent de café à l’aide de la méthode d’extraction assistée par micro-ondes à base de polyol