Name: a method for 2-photon imaging of blood flow in the neocortex through a cranial window
Uploaded: 2008-02-25T14:50:00
Description: Watch this Scientific Journal Video about A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window at JoVE.com

<p class="jove_step">尾静脉注射的右旋糖酐荧光染料</p><ol><li>在以图像脑血管，动物必须静脉注射用荧光染料。我们用右旋糖酐标记的染料，因为右旋糖酐基团，防止过大脑的血液屏障和血管渗出的染料。 </li><li>小鼠麻醉与异氟醚（归纳为4％，在注射1.5-2％）。 </li><li>尾巴是用70％酒精消毒。 </li><li>以26号针头，注入75-100微升5％V / V罗丹明右旋糖酐，生理盐水溶解的解决方案是通过尾静脉，沿尾轴的中间。 </li><li>直?...

<ol>
	<li>Kleinfeld, D., Mitra, P. P., Helmchen, F., Denk, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluctuations+and+stimulus-induced+changes+in+blood+flow+observed+in+individual+capillaries+in+layers+2+through+4+of+rat+neocortex.">Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex.</a> <em style='font-style: italic'>Proc Nat Acad Sci</em>. <b>95</b>, 15741-15746 (1998).</li><li>Pologruto, T. A., Sabatini, B. L., Svoboda, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ScanImage:+flexible+software+for+operating+laser+scanning+microscopes.">ScanImage: flexible software for operating laser scanning microscopes.</a> <em style='font-style: italic'>Biomed. Eng. Online</em>. <b>17</b>, 2-13 (2003).</li><li>Zhang, S., Murphy, T. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+the+impact+of+cortical+microcirculation+on+synaptic+structure+and+sensory-evoked+hemodynamic+responses+in+vivo.">Imaging the impact of cortical microcirculation on synaptic structure and sensory-evoked hemodynamic responses in vivo.</a> <b>5</b>, (2007).</li><li>Nishimura, N., Schaffer, C. B., Friedman, B., Lyden, P. D., Kleinfeld, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Penetrating+arterioles+are+a+bottleneck+in+the+perfusion+of+neocortex.">Penetrating arterioles are a bottleneck in the perfusion of neocortex.</a> <em style='font-style: italic'>Proc Nat Acad Sci</em>. <b>104</b>, 365-370 (2007).</li></ol>

<p>皮质血流动力学，可以研究在体内注入尾静脉鼠类与双光子显微镜的成像荧光葡聚糖染料。录像显示了如何通过玻璃覆盖的颅窗口准备在小鼠大脑皮层的图像血液血流动力学的方法。 </p>

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a method for 2-photon imaging of blood flow in the neocortex through a cranial window

<p class="jove_content">生活的啮齿类动物的完整的大脑图像的脑血管（毛细血管大型船舶）并记录血流动力学的能力是一个强大的技术。使用双光子显微镜，在体内通过颅窗口，它是可能的形象静脉注射荧光染料。这允许一个形象皮质血管，也获得血流量的测量。这种技术最初是由大卫柯菲德和温弗里德Denk。该方法可用于研究脑缺血期间或之后的血流动力学，神经退行性疾病，脑肿瘤，脑生理学或正常。例如，它已被用来研究如何中风导致血流方向转移和血红细胞的速度或梗塞和周围的磁通的变化。在这里，我们将演示如何使用双光子显微镜图像血流动力学生活用小鼠尾静脉注射荧光染料的大脑皮层。</p>

Watch this Scientific Journal Video about A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window at JoVE.com

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

<p class="jove_content">皮质血流动力学，可以研究在体内注入尾静脉鼠类与双光子显微镜的成像荧光葡聚糖染料。本视频演示了如何通过玻璃覆盖的颅窗口准备在小鼠大脑皮层的图像血液血流动力学。</p>

通过颅窗口在新皮层的血流量为双光子成像方法

The ability to image the cerebral vasculature (from large vessels to capillaries) and record blood flow dynamics in the intact brain of living rodents is ...

Research

JoVE Journal

Biology

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

夹层和小鼠的外周淋巴结的双光子成像

Two-photon imaging has revealed an elegant choreography of motility and cellular interactions within the lymph node under basal conditions and at the ...

科研

生物学

Pyrosequencing: A Simple Method for Accurate Genotyping

焦磷酸测序：一个准确的基因分型的简单方法

Pharmacogenetic research benefits first-hand from the abundance of information provided by the completion of the Human Genome Project. With such a ...

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

在胸腺的鼠标采用双光子显微镜的原位成像

Two-photon Microscopy (TPM) enables us to image deep into the thymus and document the events that are important for thymocyte development.  To follow the ...

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice

在体内2光子层钙成像对小鼠的2 / 3

To understand network dynamics of microcircuits in the neocortex, it is essential to simultaneously record the activity of a large number of neurons .  ...

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

代骨髓衍生的双光子成像使用的小鼠树突状细胞

Several methods for the preparation of murine dendritic cells can be found in the literature. Here, we present a method that produces greater than 85% ...

Dendra2 Photoswitching through the Mammary Imaging Window

通过乳腺成像窗口Dendra2 Photoswitching

In the last decade, intravital microscopy of breast tumors in mice and rats at single-cell resolution1-4  has resulted in important insights into ...

A Simple Method for Imaging <em>Arabidopsis</em> Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

A Simple Method for Imaging Arabidopsis Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium

一个影像的简单方法<em>拟南芥</em>叶浸润成像介质中使用全氟萘烷

The problem of acquiring high-resolution images deep into biological samples is widely acknowledged1. In air-filled tissue such as the spongy mesophyll of ...

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

为外植体含量的颅间充质细胞行为评定

The central nervous system is derived from the neural plate that undergoes a series of complex morphogenetic movements resulting in formation of the ...

Identification of a Murine Erythroblast Subpopulation  Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry

一个小鼠红细胞亚群富集去核活动由多光谱成像流式细胞仪鉴定

Erythropoiesis in mammals concludes with the dramatic process of enucleation that results in reticulocyte formation. The mechanism of enucleation has not ...

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

对于耐药性突变对激酶抑制剂的筛选和验证方法

The discovery of BCR/ABL as a driver oncogene in chronic myeloid leukemia (CML) resulted in the development of Imatinib, which, in fact, demonstrated the ...