Name: a craniotomy surgery procedure for chronic brain imaging
Uploaded: 2008-02-15T11:06:00
Description: Watch this Scientific Journal Video about A Craniotomy Surgery Procedure for Chronic Brain Imaging at JoVE.com

<ol><li>与异氟醚麻醉小鼠（归纳为4％，手术1.5-2％），使用IACUC核准程序。重要的是，尾巴和/或脚趾捏的使用，以确保动物是完全镇静。 </li><li>使用灭鼠微调，从颈后的头发剃的眼睛。 </li><li>鼠标放置在一个立体框架，通过手术水重新循环毯。牢固头部与耳酒吧。 </li><li>应用眼膏，以防止动物的眼睛干燥。 </li><li>管理，皮下注射，地塞米松（0.2毫克/公斤）和卡洛芬（5毫克/公斤），以防止大脑和/或炎症反应的肿...

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	<li>Svoboda, K., Denk, W., Kleinfeld, D., Tank, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=+In+vivo+dendritic+calcium+dynamics+in+neocortical+pyramidal+neurons."> In vivo dendritic calcium dynamics in neocortical pyramidal neurons.</a> Nature. 85, 161-165 (1997).</li><li>Lendvai, B., Stern, E. A., Chen, B., Svoboda, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experience-dependent+plasticity+of+dendritic+spines+in+the+developing+rat+barrel+cortex+in+vivo.">Experience-dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo.</a> Nature. 404, 876-881 (2000).</li><li>Trachtenberg, J. T., Chen, B., Knott, G. W., Feng, G., Sanes, J. R., Welker, E., Svoboda, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+in+vivo+imaging+of+experience-dependent+synaptic+plasticity+in+adult+cortex.">Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex.</a> Nature. 420, 788-794 (2002).</li><li>Portera-Cailliau, C., Weimer, R. M., DePaola, V., Caroni, P., Svoboda, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diverse+modes+of+axon+elaboration+in+the+developing+neocortex.">Diverse modes of axon elaboration in the developing neocortex.</a> PLoS Biol. 3, (2005).</li><li>Holtmaat, A., Wilbrecht, L., Knott, G. W., Welker, E., Svoboda, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experience-dependent+and+cell-type-specific+spine+growth+in+the+neocortex.">Experience-dependent and cell-type-specific spine growth in the neocortex.</a> Nature. 441, 979-983 (2006).</li></ol>

正如我们在视频和补充数字，颅窗口准备，结合双光子显微镜的使用，是一个非常强大的工具，研究新皮层的结构和功能，在体内。技术要求严格的训练，成为熟悉有关解剖和精细的手术程序，这个准备工作所需要的技能。只有原始的手术，可用于慢性成像。如果过度或刺破硬脑膜是操纵，准备不应该被用于成像。

a craniotomy surgery procedure for chronic brain imaging

在研究脑功能成像技术正变得越来越重要。其中，双光子激光扫描显微镜已成为一个非常有用的方法，因为它允许现场完整的大脑研究。有了相应的准备，这种技术可以观察相同的皮层区域的长期，从几分钟到几个月。在这段视频中，我们展示一个使用双光子显微镜的大脑成像在体内慢性准备。这种技术最初是首创的卡雷尔斯沃博达博士，谁是现在，霍华德休斯医学研究所在Janelia农场调查。像这里所显示的筹备工作，可以成像新皮层的结构（例如，树突和轴突动态），用于记录神经元的活动，使用钙敏感染料，图像皮层的血流动力学，或内在的光学成像研究。大脑皮层深处的成像是可能的最佳颅窗口手术。经营下，最可能的无菌条件下，以避免感染，使用格外小心，手术过程中不损伤硬脑膜，将导致成功和长期颅窗户玻璃覆盖。

Watch this Scientific Journal Video about A Craniotomy Surgery Procedure for Chronic Brain Imaging at JoVE.com

A Craniotomy Surgery Procedure for Chronic Brain Imaging

此视频和协议演示如何植入一个玻璃覆盖在啮齿类动物中颅窗口。这些准备工作可以用于慢性体内超过个月的时间尺度大脑皮层的双光子成像。它也可以用于其他类型的影像，包括光学内在信号成像技术。

一种慢性脑功能成像开颅手术过程

Imaging techniques are becoming increasingly important in the study brain function. Among them, two-photon laser scanning microscopy has emerged as an ...

Research

JoVE Journal

Biology

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

光固化牙科粘合树脂安装电极或微透析探针在慢性实验中的应用

In chronic recording experiments, self-curing dental acrylic resins have been used as a mounting base of electrodes or microdialysis-probes. Since these ...

科研

生物学

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

慢性的微型驱动器阵列在体内录音：驱动器的制造

Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. ...

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

慢性的微型驱动器阵列在体内录音：四极大会

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo.  The differential ...

Murine Renal Transplantation Procedure

小鼠肾移植程序

Renal orthotopic transplantation in mice is a technically challenging procedure. Although the first kidney transplants in mice were performed by Russell ...

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

现场共聚焦显微镜对斑马鱼的胚胎脑成像

In this video, we demonstrate the method our lab has developed to analyze the cell shape changes and rearrangements required to bend and fold the ...

Making MR Imaging Child's Play - Pediatric Neuroimaging Protocol, Guidelines and Procedure

使磁共振成像儿戏 - 小儿神经影像学的协议，指引及程序

Within the last decade there has been an increase in the use of structural and functional magnetic resonance imaging (fMRI) to investigate the neural ...

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

血红素加氧酶1表达上调在刮痧过程中的生物发光成像

Gua Sha is a traditional Chinese folk therapy that employs skin scraping to cause subcutaneous microvascular blood extravasation and bruises. The protocol ...

Mapping the After-effects of Theta Burst Stimulation on the Human Auditory Cortex with Functional Imaging

映射后的Theta突发刺激对人类听觉皮层功能成像

Auditory cortex pertains to the processing of sound, which is at the basis of speech or music-related processing1. However, despite considerable recent ...

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

人肝细胞通过一个两步胶原酶灌注过程隔离

The liver, an organ with an exceptional regeneration capacity, carries out a wide range of functions, such as detoxification, metabolism and homeostasis. ...

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移

Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live ...