Name: construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.
Uploaded: 2008-03-17T19:00:00
Description: Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

הקדמה עירוי ישיר של neuroactive סוכני מאפשר אזורי מוח ספציפיים כדי להיחקר תוך עקיפת מחסום דם מוח. היישומים של גישה זו במדעי המוח מגוונת הכוללת שינוי ברמה של פעילות המוח אזורי המשנה בדידים (Berretta et al, 2004;. Gliddon et al, 2005;.. קים et al, 2000), חוקרת את מ?...

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מחקרים שונים הוכיחו כי באמצעות הפחתת גודל הצינורית המסירה עבור חליטות intracerebral, טראומה רקמות עלבון מחסום דם מוח מופחתים (פרי et al., 1993), דלקת הוא ירד ואת המערכת החיסונית בתגובה נחלש (Finsen ואח&#39; דבקת., 1991), תגובתי היא פחתה (Nikkhah et al. 1994). אנו מציגים כאן שיטה להעברת כרונית של סוכני neuroactive בת...

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.
Additional Reference: 
22. Cunningham, M.G., Ames, H.M., Donalds, R.A., &amp; Benes, F.M. Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents. Journal of Neuroscience Methods 167, 213-220, doi:10.1016/j.jneumeth.2007.08.016 (2008).
Revised Abstract:
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction. (This article is based on work first reported in J Neurosci Methods. 2008 Jan 30;167(2):213-20. Epub 2007 Aug 28.).
Original Abstract:
Experimental protocols used for chronic infusion of neuroactive agents within regions of the brain often utilize a mini-osmotic pump system. Agents are commonly delivered via a stainless steel cannula with a diameter of 0.30 mm or greater. Systems utilizing a cannula of this caliber may impose trauma to the area of interest resulting in architectural damage, thereby compromising structural integrity and normal functioning. As neuroscience inquiry becomes more sophisticated, investigation of brain structures and circuitry requires improved levels of accuracy and higher resolution. We have developed a method for the preparation and implantation of a chronic infusion system within the brain utilizing a borosilicate microcannula with a tip diameter of 50 microns. This technique reduces damage to the local environment and diminishes reactive gliosis at the site of infusion. The configuration of the microinfusion system is also able to conform to the surface of the animal's skull, precluding the need for large cranial pedestals, thus facilitating closure of the scalp incision and reducing the risk of infection. We demonstrate reliable sustained delivery of a dye having a representative molecular weight using an in vitro model and in vivo studies in rats.

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.

Erratum: Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

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<td>Mini-osmotic pumps (MOPs)</td>
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<td>Electrode puller</td>
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<td>Borosilicate tubing</td>
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<td>World Precision Instruments</td>
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<td>&nbsp;</td>
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<td>Microforceps</td>
<td>Surgery</td>
<td>World Precision Instruments</td>
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construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.

פרוטוקולים ניסיוני המשמש עירוי כרונית של סוכני neuroactive בתוך אזורים במוח לעיתים קרובות לנצל מערכת מיני האוסמוטי המשאבה. סוכני מועברות בדרך כלל באמצעות צינורית נירוסטה בקוטר של 0.30 מ&quot;מ או יותר. מערכות ניצול צינורית בקוטר זה עלול להטיל טראומה לאזור הריבית וכתוצאה מכך לנזק ארכיטקטוני, ובכך לסכן את השלמות המבנית ואת לתפקוד תקין. כפי חקירה המוח הופך מתוחכם יותר, חקירה של מבנים מוחיים המעגלים דורש שיפור רמות של דיוק רזולוציה גבוהה יותר. פיתחנו שיטה להכנת ואת ההשתלה של מערכת עירוי כרונית בתוך המוח ניצול microcannula בורוסיליקט בקוטר טיפ של 50 מיקרון. טכניקה זו מפחיתה את הנזק לסביבה המקומית פוחתת דבקת תגובתי באתר של אינפוזיה. התצורה של מערכת microinfusion הוא גם מסוגל להתאים את פני השטח של הגולגולת של בעל החיים, מניעת הצורך כנים גולגולתי גדול, ובכך להקל על סגירת החתך הקרקפת וצמצום הסיכון לזיהום. אנו מדגימים משלוח מתמשכת אמין של צבע בעל משקל מולקולרי נציג שימוש במודל במבחנה במחקרים vivo בעכברים.

Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

כפי חקירה המוח הופך מתוחכם יותר, חקירה של מבנים מוחיים המעגלים דורש שיפור רמות של דיוק רזולוציה גבוהה יותר. פיתחנו שיטה להכנת ואת ההשתלה של מערכת עירוי כרונית בתוך המוח ניצול microcannula בורוסיליקט בקוטר טיפ של 50 מיקרון.

הבינוי השרשה של מערכת Microinfusion לאספקה ​​מתמשכת של סוכני Neuroactive.

Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with ...

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הבינוי השרשה של מערכת Microinfusion לאספקה ​​מתמשכת של סוכני Neuroactive.

הבינוי השרשה של מערכת Microinfusion לאספקה מתמשכת של סוכני Neuroactive.