Name: construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.
Uploaded: 2008-03-17T19:00:00
Description: Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

はじめ神経活性剤の直接注入は、血液脳関門をバイパスして、特定の脳領域を検討することができます。神経科学におけるこのアプローチのアプリケーションは多様であり、離散的なサブリージョンでの脳活動のレベルを変更することが含まれます（Berrettaら、2004;。グリッドンら、2005;。Kimら、2000。）、向精神薬の行動を調査する（クリントンら、2006;。ディベネデット?...

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様々な研究が脳内輸液、組織の外傷や血液脳関門への侮辱の配信カニューレのサイズを小さくすることで（ペリーら、1993）に減少していることが示されている、炎症が減少している免疫応答減衰（フィンセンら、1991）、及び反応性神経膠症が減少です（Nikkhahら、1994）。ここでは、従来使用されて配信カニューレのそれに比べて6倍に減少され、直径microcannulaを経由して脳内の神経活性剤の慢性的な配信の?...

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.
Additional Reference: 
22. Cunningham, M.G., Ames, H.M., Donalds, R.A., &amp; Benes, F.M. Construction and implantation of a microinfusion system for sustained delivery of neuroactive agents. Journal of Neuroscience Methods 167, 213-220, doi:10.1016/j.jneumeth.2007.08.016 (2008).
Revised Abstract:
Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with high precision while minimizing localized trauma and inflammation. Also, the ability to customize the system to accommodate animals of different species and sizes is desirable. This video presentation demonstrates the construction of an infusion system that can be fitted to any particular research animal. The delivery microcannula diameter is approximately 10-fold smaller than most infusion cannulas presently used. This translates into enhanced accuracy and reduced trauma to the brain region under study. The delivery cannula can also be sculpted to fit the contour of the surface of the animal's skull, thereby allowing closure of the scalp incision neatly over the infusion system, precluding the need for a skull-mounted pedestal, reducing risk of infection, and ensuring a greater level of comfort to the animal. The system is assembled in an air-free environment and requires the researcher to fashion glass micropipettes with a heat source. These construction methods require special skills that are best acquired, if not in person, using video instruction. (This article is based on work first reported in J Neurosci Methods. 2008 Jan 30;167(2):213-20. Epub 2007 Aug 28.).
Original Abstract:
Experimental protocols used for chronic infusion of neuroactive agents within regions of the brain often utilize a mini-osmotic pump system. Agents are commonly delivered via a stainless steel cannula with a diameter of 0.30 mm or greater. Systems utilizing a cannula of this caliber may impose trauma to the area of interest resulting in architectural damage, thereby compromising structural integrity and normal functioning. As neuroscience inquiry becomes more sophisticated, investigation of brain structures and circuitry requires improved levels of accuracy and higher resolution. We have developed a method for the preparation and implantation of a chronic infusion system within the brain utilizing a borosilicate microcannula with a tip diameter of 50 microns. This technique reduces damage to the local environment and diminishes reactive gliosis at the site of infusion. The configuration of the microinfusion system is also able to conform to the surface of the animal's skull, precluding the need for large cranial pedestals, thus facilitating closure of the scalp incision and reducing the risk of infection. We demonstrate reliable sustained delivery of a dye having a representative molecular weight using an in vitro model and in vivo studies in rats.

A correction was made to: <a href="http://www.jove.com/video/716/">Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.</a> A key reference was excluded and a revised abstract was republished.

Erratum: Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

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		<th>Material Name</th>
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<td>Mini-osmotic pumps (MOPs)</td>
<td>Tool</td>
<td>ALZET</td>
<td>model #1002</td>
<td>&nbsp;</td>
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<td>Electrode puller</td>
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<td>Stoelting Co.</td>
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<td>Borosilicate tubing</td>
<td>Surgery</td>
<td>World Precision Instruments</td>
<td>1B100F-6</td>
<td>&nbsp;</td>
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<td>Microforceps</td>
<td>Surgery</td>
<td>World Precision Instruments</td>
<td>&nbsp;</td>
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<td>Polyethylene tubing </td>
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construction and implantation of a microinfusion system for sustained delivery of neuroactive agents.

脳の領域内神経活性剤の慢性的投与のために使用される実験的なプロトコルは、しばしばミニ浸透圧ポンプシステムを採用しています。エージェントは、一般的に0.30ミリメートル以上の直径を有するステンレス鋼製カニューレを介して配信されます。この口径のカニューレを利用するシステムは、それによって構造的完全性と通常の機能を犠牲にすること、建築の被害をもたらすの関心の領域に外傷を課すことができる。神経科学の調査がより洗練さになると、脳の構造と回路の調査では、精度と高分解能の改善レベルが必要です。我々は、50ミクロンの先端径を持つホウケイ酸microcannulaを利用して脳内の慢性的な輸液システムの準備と移植する方法を開発した。この手法は、ローカル環境へのダメージを軽減し、注入部位で反応性神経膠症を減少させる。 microinfusionシステムの構成は、このように頭皮の切開の閉鎖を促進し、感染のリスクを低減、大脳神経台座の必要性を排除し、また、動物の頭蓋骨の表面に適合することができます。我々は、in vitroモデルとラットのin vivo試験で用いた代表的な分子量を有する色素の信頼性の高い持続的な配信を示しています。

Watch this Scientific Journal Video about Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents. at JoVE.com

Construction and Implantation of a Microinfusion System for Sustained Delivery of Neuroactive Agents.

神経科学の調査がより洗練さになると、脳の構造と回路の調査では、精度と高分解能の改善レベルが必要です。我々は、50ミクロンの先端径を持つホウケイ酸microcannulaを利用して脳内の慢性的な輸液システムの準備と移植する方法を開発した。

神経刺激剤の徐放のためのMicroinfusionシステムの構築と注入。

Sustained delivery of neuroactive agents is widely used in neuroscience, but poses many technical challenges. It is necessary to deliver the agent with ...

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