Name: from mefs to matrigel i: passaging hescs in the presence of mefs
Uploaded: 2008-06-04T23:27:00
Description: Watch this Scientific Journal Video about From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs at JoVE.com

<p> Embryonnaires humaines études de cellules souches dans le laboratoire Teitell sont soutenus par un California Institute for Regenerative Medicine (CIRM) Subvention de démarrage RS1-00313. Nous remercions les membres du grand centre de médecine régénérative et de recherche sur les cellules souches à l&#39;UCLA, en particulier le Dr Clark Amander, le Dr Jerome Zack, et des membres de l&#39;UCLA large souches Facilité Institut noyau cellulaire pour leur soutien de nos études. </p>

<p class="jove_title"> Fractionnement cellules souches embryonnaires humaines (CSEh) plaqué sur des fibroblastes embryonnaires de souris (MEF) </p><p class="jove_content"> Habituellement une plaque de CSEh confluentes peut être divisé 1:06-1:10, en fonction de la lignée de CSEh particulier. La plaque de répartition ne deviennent confluents nouveau 5-7 jours après la séparation. </p><ol><li> Deux jours avant de se séparer, gélatiniser plaques en utilisant une solution à 0,1% de gélatine. Pour une plaque de 6 puits, ajouter 2 ml dans c...

<ol>
	<li>Thomson, J. a. m. e. s. A., Itskovitz-Eldor, J. o. s. e. p. h., Shapiro, S. a. n. d. e. r. S., Waknitz, M. i. c. h. e. l. l. e. A., Swiergiel, J. e. n. n. i. f. e. r. J., Marshall, V. i. v. i. e. n. n. e. S., Jones, J. e. f. f. r. e. y. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Embryonic+Stem+Cell+Lines+Derived+from+Human+Blastocysts.">Embryonic Stem Cell Lines Derived from Human Blastocysts.</a> <em style='font-style: italic'>Science </em>. <b>282</b>, 1145-1147 (1998).</li><li>Xu, C. h. u. n. h. u. i., Inokuma, M. a. r. g. a. r. e. t. S., Denham, J. e. r. r. o. d., Golds, K. a. t. h. a. l. e. e. n., Kundu, P. r. a. t. i. m. a., Gold, J. o. s. e. p. h. D., Carpenter, M. e. l. i. s. s. a. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feeder-free+growth+of+undifferentiated+human+embryonic+stem+cells.">Feeder-free growth of undifferentiated human embryonic stem cells.</a> <em style='font-style: italic'>Nature Biotechnology </em>. <b>19</b>, 971-974 (2001).</li></ol>

<p> Cette vidéo montre comment faire pousser des cellules souches embryonnaires humaines (CSEh) sur les cellules de fibroblastes de souris embryonnaires d&#39;alimentation (MEF). Dans la dernière étape avant le placage, quand les CSEh sont remises en suspension, ils devraient être à peu près la taille des colonies et des formes identiques. Soigneusement pipette la suspension de CSEh haut et en bas à quelques reprises de faire des colonies plus petites et plus uniformes, mais pas tant que les cellules uniques ou très petites colonies son...

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<td>Gibco</td>
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<td>Gibco</td>
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<td>Gibco</td>
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<td>Gibco</td>
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<td>FITCI-conjugated antirabbit IgG</td>
<td>Reagent</td>
<td>Jackson ImmunoResearch Laboratories. Inc.</td>
<td>715-095-152</td>
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from mefs to matrigel i: passaging hescs in the presence of mefs

<p class="jove_content">Cette vidéo montre comment faire pousser des cellules souches embryonnaires humaines (CSEh) sur les cellules de fibroblastes de souris embryonnaires d&#39;alimentation (MEF).</p>

Watch this Scientific Journal Video about From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs at JoVE.com

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

De MEFs à I Matrigel: CSEh repiquage en présence du MEF

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.
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Research

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Biology

Passaging Human Neural Stem Cells

Le repiquage des cellules souches humaines

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In recent years, stem cell research has led to a better understanding of developmental biology, various diseases and its potential impact on regenerative ...

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Over the past several decades, a tube formation assay using growth factor-reduced Matrigel has been typically employed to demonstrate the angiogenic ...

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Generation of a homogeneous and abundant population of skeletal muscle cells from human embryonic stem cells (hESCs) is a requirement for cell-based ...

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