Name: from mefs to matrigel i: passaging hescs in the presence of mefs
Uploaded: 2008-06-04T23:27:00
Description: Watch this Scientific Journal Video about From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs at JoVE.com

<p> Embrionali umane studi sulle cellule staminali in laboratorio Teitell sono supportati da una California Institute for Regenerative Medicine (CIRM) sementi concedere RS1-00313. Ringraziamo i membri del Centro di Medicina Rigenerativa Broad e ricerca sulle cellule staminali presso la UCLA, in particolare il Dott. Amander Clark, il Dr. Jerome Zack, e membri del Fondo per la UCLA Stem Cell Institute Broad core per il loro sostegno dei nostri studi. </p>

<p class="jove_title"> Dividere le cellule staminali embrionali umane (hESC) placcato al passaggio del mouse fibroblasti embrionali (MEF) </p><p class="jove_content"> Di solito un piatto hESC confluenti possono essere divisi 1:06-1:10, a seconda della linea hESC particolare. Il piatto sarà diviso confluenti ancora 5-7 giorni dopo la divisione. </p><ol><li> Due giorni prima della scissione, gelatinizzare piatti utilizzando una soluzione 0,1% di gelatina. Per un 6-pozzetti, aggiungere 2 ml in ogni piatto e incubare a 37 ° C, 5% CO <sub>2</sub> ...

<ol>
	<li>Thomson, J. a. m. e. s. A., Itskovitz-Eldor, J. o. s. e. p. h., Shapiro, S. a. n. d. e. r. S., Waknitz, M. i. c. h. e. l. l. e. A., Swiergiel, J. e. n. n. i. f. e. r. J., Marshall, V. i. v. i. e. n. n. e. S., Jones, J. e. f. f. r. e. y. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Embryonic+Stem+Cell+Lines+Derived+from+Human+Blastocysts.">Embryonic Stem Cell Lines Derived from Human Blastocysts.</a> <em style='font-style: italic'>Science </em>. <b>282</b>, 1145-1147 (1998).</li><li>Xu, C. h. u. n. h. u. i., Inokuma, M. a. r. g. a. r. e. t. S., Denham, J. e. r. r. o. d., Golds, K. a. t. h. a. l. e. e. n., Kundu, P. r. a. t. i. m. a., Gold, J. o. s. e. p. h. D., Carpenter, M. e. l. i. s. s. a. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feeder-free+growth+of+undifferentiated+human+embryonic+stem+cells.">Feeder-free growth of undifferentiated human embryonic stem cells.</a> <em style='font-style: italic'>Nature Biotechnology </em>. <b>19</b>, 971-974 (2001).</li></ol>

<p> Questo video dimostra come far crescere cellule staminali embrionali umane (hESC) sulle cellule dei fibroblasti del mouse (MEF) le cellule di alimentazione. Nell&#39;ultimo passaggio prima della placcatura, quando le hESC sono risospesi, dovrebbero essere di dimensioni della colonia o meno uniforme e la forma. Con attenzione pipetta la sospensione hESC su e giù per un paio di volte a fare le colonie più piccole e più uniformi, ma non così tanto che le singole cellule o colonie sono molto piccole macchie generated.Immunofluorescence e ci...

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from mefs to matrigel i: passaging hescs in the presence of mefs

<p class="jove_content">Questo video dimostra come far crescere cellule staminali embrionali umane (hESC) sulle cellule dei fibroblasti del mouse (MEF) le cellule di alimentazione.</p>

Watch this Scientific Journal Video about From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs at JoVE.com

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

<p class="jove_content">Questo video dimostra come far crescere cellule staminali embrionali umane (hESC) sulle cellule dei fibroblasti del mouse (MEF) le cellule di alimentazione. Parte 1 di 3.</p>

Dal MEF per Matrigel I: hESC Passaging in presenza di MEF

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.
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