Name: from mefs to matrigel i: passaging hescs in the presence of mefs
Uploaded: 2008-06-04T23:27:00
Description: Watch this Scientific Journal Video about From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs at JoVE.com

<p> Teitellラボでヒト胚性幹細胞研究は再生医療のためのカリフォルニア工科大学（CIRM）種子助成RS1 - 00313でサポートされています。我々は、UCLA、特に博士Amanderクラーク博士ジェロームザック、そして我々の研究の支援のためのUCLAのブロード研究所の幹細胞の中核施設のメンバーで、再生医療と幹細胞研究のブロードセンターのメンバーに感謝。 </p>

<p class="jove_title">マウス胚性繊維芽細胞（MEF）上に播種した分割するヒト胚性幹細胞（ヒトES） </p><p class="jove_content">通常コンフルエントhESCのプレートは、特定のhESCのラインに応じて、1:10に1時06分を分割することができます。分割板が分割後に再度5〜7日間コンフルエントになります。 </p><ol><li>分割の前に2日間、0.1％ゼラチン溶液を用いてプレートをゼラチン状にする。 6ウェルプレートの場合は、一晩37℃、...

<ol>
	<li>Thomson, J. a. m. e. s. A., Itskovitz-Eldor, J. o. s. e. p. h., Shapiro, S. a. n. d. e. r. S., Waknitz, M. i. c. h. e. l. l. e. A., Swiergiel, J. e. n. n. i. f. e. r. J., Marshall, V. i. v. i. e. n. n. e. S., Jones, J. e. f. f. r. e. y. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Embryonic+Stem+Cell+Lines+Derived+from+Human+Blastocysts.">Embryonic Stem Cell Lines Derived from Human Blastocysts.</a> <em style='font-style: italic'>Science </em>. <b>282</b>, 1145-1147 (1998).</li><li>Xu, C. h. u. n. h. u. i., Inokuma, M. a. r. g. a. r. e. t. S., Denham, J. e. r. r. o. d., Golds, K. a. t. h. a. l. e. e. n., Kundu, P. r. a. t. i. m. a., Gold, J. o. s. e. p. h. D., Carpenter, M. e. l. i. s. s. a. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feeder-free+growth+of+undifferentiated+human+embryonic+stem+cells.">Feeder-free growth of undifferentiated human embryonic stem cells.</a> <em style='font-style: italic'>Nature Biotechnology </em>. <b>19</b>, 971-974 (2001).</li></ol>

<p>このビデオでは、マウス胚性線維芽細胞（MEF）フィーダー細胞上でヒト胚性幹細胞（ヒトES）を成長させる方法を示しています。メッキ前の最後のステップでは、ヒトES細胞が再懸濁するとき、彼らはほぼ均一なコロニーの大きさと形状でなければならない。慎重にコロニーが小さく、より均一にするため、上下に数回hESCの懸濁液をピペットではなく、あまり単一の細胞または非常に小さいコロニーのよう?...

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<td>Gibco</td>
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<td>Gibco</td>
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<td>Gibco</td>
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<td>Jackson ImmunoResearch Laboratories. Inc.</td>
<td>715-095-152</td>
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from mefs to matrigel i: passaging hescs in the presence of mefs

<p class="jove_content">このビデオでは、マウス胚性線維芽細胞（MEF）フィーダー細胞上でヒト胚性幹細胞（ヒトES）を成長させる方法を示しています。</p>

Watch this Scientific Journal Video about From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs at JoVE.com

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

<p class="jove_content">このビデオでは、マウス胚性線維芽細胞（MEF）フィーダー細胞上でヒト胚性幹細胞（ヒトES）を成長させる方法を示しています。 3のパート1。</p>

MEFの存在下で継代ヒトES細胞：MEFをから私をマトリゲルする

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.
...

Research

JoVE Journal

Biology

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Pluripotency can be induced in differentiated murine by viral transduction of Oct4, Sox2, Klf4, and c-Myc (Takahashi and Yamanaka, 2006;  Wernig, et al., ...

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This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF ...

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This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage ...

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

腫瘍細胞のVasculogenic活性を評価するためにマトリゲルベースの​​チューブ形成アッセイ

Over the past several decades, a tube formation assay using growth factor-reduced Matrigel has been typically employed to demonstrate the angiogenic ...

Generation of Myospheres From hESCs by Epigenetic Reprogramming

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Generation of a homogeneous and abundant population of skeletal muscle cells from human embryonic stem cells (hESCs) is a requirement for cell-based ...

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Dysfunctional skeletal muscle mitochondria play a role in altered metabolism observed with aging, obesity and Type II diabetes. Mitochondrial ...

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天然化合物の存在下でそのまま β 細胞のミトコンドリア機能を測定するための高解像度の呼吸

High-resolution respirometry allows for the measurement of oxygen consumption of isolated mitochondria, cells and tissues. Beta cells play a critical role ...

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ショ<em>ウジョウバエ</em>における作物運動性と食物過大評価の測定

Most animals use the gastrointestinal (GI) tract to digest food. The movement of the ingested food in the GI tract is essential for nutrient absorption. ...