Name: non-invasive 3d-visualization with sub-micron resolution using synchrotron-x-ray-tomography
Uploaded: 2008-05-27T00:00:00
Description: Watch this Scientific Journal Video about Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography at JoVE.com

我们感谢他们协助在ESRF帕沃劳曼，伯格曼，迈克尔和塞巴斯蒂安Schmelzle。这项工作是支持欧洲同步辐射设施项目通过光束的时间分配的SC - 2127。

在这项研究中使用的动物从我们的实验室培养的孤雌生殖oribatid螨Archegozetes longisetosus（蜱螨亚纲，Oribatida）取标本。文化的增长在不断的黑暗巴黎/塑料罐子木炭混合（9:1）的石膏，在20-23℃，空气湿度约90％。 样品制备<ol><li>取标本，从文化，用细刷清洗，并放置在一个6:3:1混合80％乙醇，35％的甲醛和24小时100％的醋酸。 </li><li>随后，标本脱水，70％的乙?...

<ol>
	<li>Betz, O., Wegst, U., Weide, D., Heethoff, M., Helfen, L., Lee, W. -. K., Cloetens, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+applications+of+synchrotron+X-ray+phase-contrast+microtomography+in+biological+morphology+and+biomaterial+science.+I.+General+aspects+of+the+technique+and+its+advantages+in+the+analysis+of+millimetre-sized+arthropod+structure.">Imaging applications of synchrotron X-ray phase-contrast microtomography in biological morphology and biomaterial science. I. General aspects of the technique and its advantages in the analysis of millimetre-sized arthropod structure.</a> J. Microscopy. 22, 51-71 (2007).</li><li>Cloetens, P., Barrett, R., Baruchel, J., Guigay, J. P., Schlenker, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phase+objects+in+synchrotron+radiation+hard+X-ray+imaging.">Phase objects in synchrotron radiation hard X-ray imaging.</a> J. Phys. D: Appl. Phys. 29, 133-146 (1996).</li><li>Cloetens, P., Pateyron-Salome, M., Buffiere, J. Y., Peix, G., Baruchel, J., Peyrin, V., Schlenker, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Observation+in+microstructure+and+damage+in+materials+by+phase+sensitive+radiography+and+tomography.">Observation in microstructure and damage in materials by phase sensitive radiography and tomography.</a> J. Apll. Phys. 81, 5878-5886 (1997).</li><li>Clotens, P., Ludwig, W., Baruchel, J., van Dyck, D., van Landyut, J., Guigay, J. P., Schlenker, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Holotomography:+quantitative+phase+tomography+with+micrometer+resolution+using+hard+synchrotron+radiation+X-rays.">Holotomography: quantitative phase tomography with micrometer resolution using hard synchrotron radiation X-rays.</a> Appl. Phys. Lett. 75, 2912-2914 (1999).</li><li>Heethoff, M., Cloetens, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Comparison+of+aynchrotron+X-ray+phase+contrast+tomography+and+holotomography+for+non-invasive+investigations+of+the+internal+anatomy+of+mites.">A Comparison of aynchrotron X-ray phase contrast tomography and holotomography for non-invasive investigations of the internal anatomy of mites.</a> Soil Organisms. , (2008).</li></ol>

在此演示中，我们集中在一个chelicerate微节肢动物的内部解剖的三维可视化。同步辐射X射线测量允许的像素分辨率为0.3微米，取决于样本的大小。在这里，我们已经表明0.7μm像素分辨率的数据。一般情况下，同步辐射X射线断层扫描可用于分析X射线的衰减低的小生物材料（或组织）。像素的分辨率几乎达到了传统的光学显微镜。该技术可以适用于任何一种材料内部组织的利益，必须不能被切割销毁。然而，组织?...

non-invasive 3d-visualization with sub-micron resolution using synchrotron-x-ray-tomography

鲜为人知的是，有关的许多微节肢动物的身体尺寸小于1毫米的内部组织。原因是体积小，硬的角质层，这使得它很难使用古典组织学的协议。此外，组织学切片破坏样品，因此无法使用独特的材料。因此，非破坏性的方法是可取的，可以查看里面的小样本，而不需要切片。 我们用同步辐射X射线断层扫描在格勒诺布尔（法国）的欧洲同步辐射装置（ESRF）的非侵入性产生了0.7μm的像素分辨率的三维断层数据集。使用量的渲染软件，这使我们能够重建无病理切片制作的文物在自然状态下的内部组织。这些日期可以用于定量形态，地标，或可视化的动画电影，了解隐藏的身体部位的结构，并遵循通过样品的完整的器官系统或组织。

Watch this Scientific Journal Video about Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography at JoVE.com

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

我们使用的欧洲同步辐射装置（ESRF）的同步辐射X射线断层检查，非侵入性产生了0.7μm的像素分辨率的三维断层数据集。使用量的渲染软件，这使得无病理切片制作的文物在自然状态下的内部结构重建。

非侵入性的三维可视化与亚微米分辨率使用同步加速器X射线断层扫描

Little is known about the internal organization of many micro-arthropods with body sizes below 1 mm. The reasons for that are the small size and the hard ...

Research

JoVE Journal

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科研

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Striated muscle cells are indispensable for the activity of humans and animals. Single muscle fibers are comprised of myofibrils, which consist of ...