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DOI :
10.3791/745-v
July 23rd, 2008
Chapters
0:00
Introduction
0:08
Equipment
0:24
Materials
1:24
Preparation of HBSS/HSA
2:06
Blood sample
2:38
Stages of neutrophil isolation
2:53
First separation: acquire neutrophils
4:56
Second separation: refine neutrophil layer
5:39
Third separation: lysing the red blood cells
6:27
Fourth separation: resuspending the neutrophils
好中球は炎症性免疫応答のサイトに到着する最初のセルの一つです、そしてその機能とメカニズムをin vitroで広く研究されている。我々は、市販の分離媒体を用いて全血からヒト好中球を分離するために標準の密度勾配分離法を示しています。
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