Name: counting and determining the viability of cultured cells
Uploaded: 2008-06-23T15:41:00
Description: Watch this Scientific Journal Video about Counting and Determining the Viability of Cultured Cells at JoVE.com

这个实验方法的完整的文本协议<a href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb0101/current/abstract" target="_blank">是可在细胞生物学</a>中的电流协议。

counting and determining the viability of cultured cells

重要的是，在培养条件的标准化和进行准确的定量实验确定文化中的细胞数量。一个hemacytometer是与中心区作为计数室设计了一个厚厚的玻璃幻灯片。适用于某个特定地区的细胞悬液，计数，这样可以计算出细胞密度。

Watch this Scientific Journal Video about Counting and Determining the Viability of Cultured Cells at JoVE.com

Counting and Determining the Viability of Cultured Cells

重要的是，在培养条件的标准化和进行准确的定量实验确定文化中的细胞数量。在这段视频中，我们展示了如何使用一个hemacytometer细胞计数。

计数和确定培养细胞的可行性

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A ...

Research

JoVE Journal

Biology

Counting Human Neural Stem Cells

人类神经干细胞计数

Knowledge of the exact number of viable cells in a given volume of a cell suspension is required for many routine tissue culture manipulations, such as ...

科研

生物学

Determining the Contribution of the Energy Systems During Exercise

确定的能源系统，在运动的贡献

One of the most important aspects of the metabolic demand is the relative contribution of the energy systems to the total energy required for a given ...

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

GFP的表达和使用的大里基于图像流式细胞仪的可行性评估

Single-cell and population information are commonly obtained either by flow cytometry or fluorescence microscopy. However, these two methods provide ...

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

评估肾细胞​​蛋白激酶C同工酶，线粒体功能及细胞活力的

The protein kinase C (PKC) family of isozymes is involved in numerous physiological and pathological processes. Our recent data demonstrate that PKC ...

Chromosome Preparation From Cultured Cells

染色体制备培养细胞

Chromosome (cytogenetic) analysis is widely used for the detection of chromosome instability. When followed by G-banding and molecular techniques such as ...

Viability Assays for Cells in Culture

活性检测在培养细胞

Manual cell counts on a microscope are a sensitive means of assessing cellular viability but are time-consuming and therefore expensive. Computerized ...

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

内质网子域的培养细胞中的可视化

The lipids and proteins in eukaryotic cells are continuously exchanged between cell compartments, although these retain their distinctive composition and ...

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

快速，特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定

Vitamin C (ascorbate) plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. For instance, within ...

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

隔离和线粒体从培养细胞和小鼠组织功能分析

Comparison between two or more distinct groups, such as healthy vs. disease, is necessary to determine cellular status. Mitochondria are at the nexus of ...

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

成像本地CA<sup&gt; 2+</sup&gt;在培养的哺乳动物细胞信号

Cytosolic Ca2+ ions regulate numerous aspects of cellular activity in almost all cell types, controlling processes as wide-ranging as gene transcription, ...