Name: trypsinizing and subculturing mammalian cells
Uploaded: 2008-06-12T13:28:00
Description: Watch this Scientific Journal Video about Trypsinizing and Subculturing Mammalian Cells at JoVE.com

この実験的なアプローチのための完全なテキストのプロトコルでは、利用可能です<a href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb0101/current/abstract" target="_blank">細胞生物学におけるカレントプロトコール</a> 。

trypsinizing and subculturing mammalian cells

細胞がコンフルエントに達するように、彼らは、継代培養や継代する必要があります。最終的には減少分裂指数と細胞死の中のサブカルチャーコンフルエントの細胞の結果に障害が発生。継代培養の最初のステップは、トリプシン処理や機械的手段によって初代培養容器の表面から細胞を剥離することです。結果として細胞懸濁液は、細分化、または新鮮な培養液に、再接種される。二次培養物は定期的に成長し、供給のためにチェックされ、続いて三次の文化を生成するために継代培養することができます。細胞の継代の間の時間は細胞株によって変化し、成長率に依存します。

Watch this Scientific Journal Video about Trypsinizing and Subculturing Mammalian Cells at JoVE.com

Trypsinizing and Subculturing Mammalian Cells

細胞がコンフルエントに達するように、彼らは、継代培養や継代する必要があります。このビデオでは、接着および浮遊細胞の両方を継代培養するための手順をデモンストレーションを行います。

哺乳動物細胞をトリプシン処理し、継代培養

As cells reach confluency, they must be subcultured or passaged. Failure to subculture confluent cells results in reduced mitotic index and eventually in ...

Research

JoVE Journal

Biology

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

広視野顕微鏡を用いて長期的なイメージング哺乳類細胞

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

接着および非接着性の哺乳動物細胞の in situ細胞下分画で

Protein function is intimately coupled to protein localization. Although some proteins are restricted to a specific location or subcellular compartment, ...

MISSION esiRNA for RNAi Screening in Mammalian Cells

哺乳動物細胞におけるRNAiスクリーニングのためのミッションesiRNA

RNA interference (RNAi) is a basic cellular mechanism for the control of gene expression. RNAi is induced by short double-stranded RNAs also known as ...

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

異種哺乳動物細胞で発現したイオンチャネルの変異導入と機能解析

We will demonstrate how to study the functional effects of introducing a point mutation in an ion channel. We study G protein-gated inwardly rectifying ...

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

ミッションLentiPlexは、哺乳動物細胞ではshRNAライブラリースクリーニングをプールされた

RNA interference (RNAi) is an intrinsic cellular mechanism for the regulation of gene expression. Harnessing the innate power of this system enables us to ...

Analysis of Cell Cycle Position in Mammalian Cells

哺乳動物細胞における細胞周期の位置の解析

The regulation of cell proliferation is central to tissue morphogenesis during the development of multicellular organisms. Furthermore, loss of control of ...

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

哺乳動物細胞における小胞体ローカライズされたmRNAの可視化

In eukaryotes, most of the messenger RNAs (mRNAs) that encode secreted and membrane proteins are localized to the surface of the endoplasmic reticulum ...

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

細胞毒性の継続とリアルタイム監視のための自律的に生物発光哺乳類細胞

Mammalian  cell-based in vitro assays have been  widely employed as alternatives to animal testing for toxicological studies but  have been limited due to ...

Measurement of Heme Synthesis Levels in Mammalian Cells

哺乳動物細胞におけるヘム合成レベルの測定

Heme serves as the prosthetic group for a wide variety of proteins known as hemoproteins, such as hemoglobin, myoglobin and cytochromes. It is involved in ...

Massively Parallel Reporter Assays in Cultured Mammalian Cells

培養哺乳動物細胞での超並列レポーターアッセイ

The genetic reporter assay is a well-established and powerful tool for dissecting the relationship between DNA sequences and their gene regulatory ...