Name: freezing, thawing, and packaging cells for transport
Uploaded: 2008-07-02T15:30:00
Description: Watch this Scientific Journal Video about Freezing, Thawing, and Packaging Cells for Transport at JoVE.com

这个实验方法的完整的文本协议<a class="jove_content" href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb0101/current/abstract">是可在细胞生物学中</a>的电流协议。

freezing, thawing, and packaging cells for transport

培养的哺乳动物细胞在细胞生物学研究中被广泛使​​用。它需要一些特殊技能，以便能够保留的结构，功能，行为和文化的细胞生物学。本视频介绍了冻结和存储细胞，以及如何恢复冻结的股票所需的基本技能。

Watch this Scientific Journal Video about Freezing, Thawing, and Packaging Cells for Transport at JoVE.com

Freezing, Thawing, and Packaging Cells for Transport

培养的哺乳动物细胞在细胞生物学研究中被广泛使​​用。本视频介绍了冻结和存储细胞，以及如何恢复冻结的股票所需的基本技能。

冷冻，解冻，和运输包装细胞

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the ...

Research

JoVE Journal

Biology

Freezing Human ES Cells

冻结人类胚胎干细胞

Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.  A healthy, exponentially expanding culture is washed with PBS to remove ...

科研

生物学

Flash Freezing and Cryosectioning E12.5 Mouse Brain

闪存冻结和Cryosectioning E12.5小鼠脑

Freezing and Thawing Human Embryonic Stem Cells

人类胚胎干细胞冷冻和解冻

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

细胞器运输的培养<em&gt;果蝇</em&gt;细胞：S2细胞系和原代神经元。

Drosophila S2 cells plated on a coverslip in the presence of any actin-depolymerizing drug form long unbranched processes filled with uniformly polarized ...

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

组织分流和冻结的骨骼肌疾病模型

Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal ...

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

串联高压冷冻和植物组织中快速冷冻替代透射电子显微镜

Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, ...

Using Caco-2 Cells to Study Lipid Transport by the Intestine

采用Caco-2细胞研究脂质运输由肠

Studies of dietary fat absorption are generally conducted by using an animal model equipped with a lymph cannula. Although this animal model is widely ...

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

肾毒素显微注射斑马鱼到模型急性肾损伤

The kidneys are susceptible to harm from exposure to chemicals they filter from the bloodstream. This can lead to organ injury associated with a rapid ...

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

暴跌冻结：在透射电子显微镜的超微结构和免疫组化研究工具悬架细胞

Transmission Electron Microscopy (TEM) is an extraordinary tool for studying cell ultrastructure, in order to localize proteins and visualize ...

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入

Studies on skeletal muscle physiology face the technical challenge of appropriately processing the specimens to obtain sections with clearly visible ...