Name: freezing, thawing, and packaging cells for transport
Uploaded: 2008-07-02T15:30:00
Description: Watch this Scientific Journal Video about Freezing, Thawing, and Packaging Cells for Transport at JoVE.com

この実験的なアプローチのための完全なテキストのプロトコルでは、利用可能です<a class="jove_content" href="http://www.mrw.interscience.wiley.com/emrw/9780471143031/cp/cpcb/article/cb0101/current/abstract">細胞生物学におけるカレントプロトコール。</a>

freezing, thawing, and packaging cells for transport

培養哺乳動物細胞は、細胞生物学の研究で​​広く使用されています。それは構造、機能、行動、及び培養中の細胞の生物学を維持できるようにするために特別なスキルの数が必要です。このビデオでは、細胞とどのように凍結ストックを回復するために凍結保存するために必要な基本的なスキルを説明します。

Watch this Scientific Journal Video about Freezing, Thawing, and Packaging Cells for Transport at JoVE.com

Freezing, Thawing, and Packaging Cells for Transport

培養哺乳動物細胞は、細胞生物学の研究で​​広く使用されています。このビデオでは、細胞とどのように凍結ストックを回復するために凍結保存するために必要な基本的なスキルを説明します。

トランスポートのための凍結、融解、およびパッケージング細胞

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the ...

Research

JoVE Journal

Biology

Freezing Human ES Cells

凍結ヒトES細胞

Here we demonstrate how our lab freezes HuES human embryonic stem cell lines.  A healthy, exponentially expanding culture is washed with PBS to remove ...

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Flashは、E12.5マウス脳の凍結とCryosectioning

Freezing and Thawing Human Embryonic Stem Cells

ヒト胚性幹細胞を凍結融解

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

培養中の細胞小器官輸送<em&gt;ショウジョウバエ</em&gt;細胞：S2細胞ラインと一次ニューロン。

Drosophila S2 cells plated on a coverslip in the presence of any actin-depolymerizing drug form long unbranched processes filled with uniformly polarized ...

Tissue Triage and Freezing for Models of Skeletal Muscle Disease

組織トリアージや骨格筋疾患のモデルのために凍結

Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal ...

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

タンデム高圧凍結および透過型電子顕微鏡のための植物組織の急速凍結置換

Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, ...

Using Caco-2 Cells to Study Lipid Transport by the Intestine

腸によって脂質輸送を研究するためのCaco-2細胞を用いました

Studies of dietary fat absorption are generally conducted by using an animal model equipped with a lymph cannula. Although this animal model is widely ...

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

ゼブラフィッシュにおける腎毒素マイクロインジェクションは急性腎障害をモデルに

The kidneys are susceptible to harm from exposure to chemicals they filter from the bloodstream. This can lead to organ injury associated with a rapid ...

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

プランジ凍結：透過型電子顕微鏡での懸濁細胞の超微細構造と免疫学的研究のためのツール

Transmission Electron Microscopy (TEM) is an extraordinary tool for studying cell ultrastructure, in order to localize proteins and visualize ...

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

筋肉組織を直接液体窒素中に浸漬されたときにアーティファクトを凍結マルチホールクライオバイアルが不要

Studies on skeletal muscle physiology face the technical challenge of appropriately processing the specimens to obtain sections with clearly visible ...