Name: electrophoretic separation of proteins
Uploaded: 2008-06-12T13:29:00
Description: Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

这个实验方法的完整的文本<a href="http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/mb1002a/current/abstract" target="_blank">协议，可用在分子</a>生物学中的电流协议。

electrophoretic separation of proteins

电泳用于分离复杂的蛋白质混合物（例如，从细胞，亚细胞组分，列分数，或免疫），调查亚基组成，并验证蛋白质样品的同质性。 ，它也可以用来净化在进一步的应用中使用的蛋白质。在聚丙烯酰胺凝胶电泳，蛋白质通过毛孔中的聚丙烯酰胺凝胶基质中的电场响应迁移;孔径丙烯酰胺浓度的增加而降低。孔径和蛋白质的电荷，大小和形状的组合决定了蛋白质的迁移率。在这个单元，标准的Laemmli方法描述不连续的凝胶电泳变性条件下，也就是说，在十二烷基硫酸钠（SDS），的存在。

Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

Electrophoretic Separation of Proteins

在这段视频中，我们展示了使用acrylimide聚凝胶电泳（PAGE）蛋白电泳分离的方法。

电泳分离蛋白质

Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to ...

Research

JoVE Journal

Biology

Staining Proteins in Gels

在凝胶染色蛋白质

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit describes protocols for ...

科研

生物学

Microcontact Printing of Proteins for Cell Biology

微接触印刷的蛋白细胞生物学

The ability to pattern proteins and other biomolecules onto substrates is important for capturing the spatial complexity of the extracellular environment. ...

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

交配和四分体分离衣藻遗传分析

The unicellular green alga Chlamydomonas reinhardtii (Chlamydomonas) has become a popular organism for research in diverse areas of cell biology and ...

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs

分离和富集大鼠间质干细胞（MSCS）及衍生的干细胞分离单细胞集落

MSCs are a population of adult stem cells that is a promising source for therapeutic applications. These cells can be isolated from the bone marrow and ...

Crystallization of Membrane Proteins in Lipidic Mesophases

在油脂介孔膜蛋白的结晶

Membrane proteins perform critical functions in living cells related to signal transduction, transport and energy transformations, and, as such, are ...

Pull-down of Calmodulin-binding Proteins

钙调蛋白结合蛋白的下拉

Calcium (Ca2+) is an ion vital in regulating cellular function through a variety of mechanisms. Much of Ca2+ signaling is mediated through the ...

Agarose Gel Electrophoresis for the Separation of DNA Fragments

琼脂糖凝胶电泳分离DNA片段

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Agarose is isolated from ...

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

分离的小鼠胚胎面外胚层和间充质

 Orofacial clefts are the most frequent  craniofacial defects, which affect 1.5 in 1,000 newborns worldwide1,2. Orofacial clefting is caused by abnormal ...

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

生精细胞类型使用STA把速度沉降分离

Mammalian spermatogenesis is a complex  differentiation process that occurs in several stages in the seminiferous  tubules of the testes. Currently, there ...

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

凝胶电泳迁移分析（EMSA）为RNA，蛋白质相互作用的研究：IRE / IRP示例

RNA/protein interactions are critical for post-transcriptional regulatory pathways. Among the best-characterized cytosolic RNA-binding proteins are iron ...