Name: electrophoretic separation of proteins
Uploaded: 2008-06-12T13:29:00
Description: Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

Der vollständige Text Protokoll für diesen experimentellen Ansatz ist in <a href="http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/mb1002a/current/abstract" target="_blank">Current Protocols in Molecular Biology</a> .

electrophoretic separation of proteins

Elektrophorese wird verwendet, um komplexe Gemische von Proteinen (z. B. von Zellen, subzellulären Fraktionen, Säulenfraktionen oder Immunpräzipitaten) zu trennen, die Untereinheit Kompositionen zu untersuchen und um die Homogenität der Protein-Proben zu überprüfen. Es kann auch dazu dienen, um Proteine ​​für den Einsatz in weiteren Anwendungen zu reinigen. In Polyacrylamidgelelektrophorese, Proteine ​​in Reaktion auf ein elektrisches Feld durch die Poren in einem Polyacrylamid-Gel-Matrix zu migrieren, Porengröße nimmt mit steigender Acrylamid-Konzentration. Die Kombination von Porengröße und Protein Ladung, Größe und Form bestimmt die Wanderungsgeschwindigkeit des Proteins. In dieser Einheit ist die Standard-Laemmli-Methode für die diskontinuierliche Gelelektrophorese unter denaturierenden Bedingungen beschrieben, dh in Gegenwart von Natriumdodecylsulfat (SDS).

Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

Electrophoretic Separation of Proteins

In diesem Video zeigen wir eine Methode zur elektrophoretischen Trennung von Proteinen unter Verwendung von Poly-acrylimid Gelelektrophorese (PAGE).

Elektrophoretische Trennung von Proteinen

Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to ...

Research

JoVE Journal

Biology

Staining Proteins in Gels

Die Färbung Proteine ​​in Gelen

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit describes protocols for ...

Forschung

Biologie

Microcontact Printing of Proteins for Cell Biology

Mikrokontaktdrucken von Proteinen für Zellbiologie

The ability to pattern proteins and other biomolecules onto substrates is important for capturing the spatial complexity of the extracellular environment. ...

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Paarung und Tetrad Trennung von Chlamydomonas reinhardtii Für genetische Analysen

The unicellular green alga Chlamydomonas reinhardtii (Chlamydomonas) has become a popular organism for research in diverse areas of cell biology and ...

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs

Isolierung und Anreicherung von Rat mesenchymalen Stammzellen (MSCs) und Separation von Single-Kolonie Derived MSCs

MSCs are a population of adult stem cells that is a promising source for therapeutic applications. These cells can be isolated from the bone marrow and ...

Crystallization of Membrane Proteins in Lipidic Mesophases

Die Kristallisation von Membranproteinen in Lipidische Mesophasen

Membrane proteins perform critical functions in living cells related to signal transduction, transport and energy transformations, and, as such, are ...

Pull-down of Calmodulin-binding Proteins

Pull-down von Calmodulin-bindende Proteine

Calcium (Ca2+) is an ion vital in regulating cellular function through a variety of mechanisms. Much of Ca2+ signaling is mediated through the ...

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarosegel-Elektrophorese für die Trennung von DNA-Fragmenten

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Agarose is isolated from ...

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Trennung von embryonalen Maus-Facial Ektoderm und Mesenchym

 Orofacial clefts are the most frequent  craniofacial defects, which affect 1.5 in 1,000 newborns worldwide1,2. Orofacial clefting is caused by abnormal ...

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Die Trennung der Spermatogenese Zelltypen Mit STA-PUT Velocity Sedimentation

Mammalian spermatogenesis is a complex  differentiation process that occurs in several stages in the seminiferous  tubules of the testes. Currently, there ...

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

Electrophoretic Mobility Shift Assay (EMSA) für das Studium von RNA-Protein-Wechselwirkungen: Die IRE / IRP Beispiel

RNA/protein interactions are critical for post-transcriptional regulatory pathways. Among the best-characterized cytosolic RNA-binding proteins are iron ...