Name: electrophoretic separation of proteins
Uploaded: 2008-06-12T13:29:00
Description: Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

この実験的なアプローチのための完全なテキストのプロトコルでは使用可能です<a href="http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/mb1002a/current/abstract" target="_blank">分子生物学におけるカレントプロトコール</a> 。

electrophoretic separation of proteins

電気泳動はタンパク質の複雑な混合物を分離する（例えば、細胞、細胞内の分画、カラム画分、または免疫沈降から）、サブユニットの組成を調査するため、およびタンパク質サンプルの均質性を検証するために使用されます。また、さらにアプリケーションで使用するためのタンパク質を精製するのに役立つことができる。ポリアクリルアミドゲル電気泳動では、蛋白質はポリアクリルアミドゲルマトリックスの細孔を通して電界に反応して移行し、細孔サイズが増加するアクリルアミドの濃度が減少する。細孔径と蛋白質の電荷、大きさ、および形状の組み合わせは、タンパク質の移行速度を決定します。このユニットでは、標準をLaemmli法は、ドデシル硫酸ナトリウム（SDS）の存在下で、変性条件下で不連続ゲル電気泳動のために、すなわち記述されている。

Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

Electrophoretic Separation of Proteins

このビデオでは、我々は、ポリ- acrylimideゲル電気泳動（PAGE）を用いてタンパク質の電気泳動分離する方法を示しています。

タンパク質の電気泳動分離

Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to ...

Research

JoVE Journal

Biology

Staining Proteins in Gels

ゲルでタンパク質を染色

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit describes protocols for ...

Microcontact Printing of Proteins for Cell Biology

細胞生物学のためのタンパク質のマイクロコンタクトプリンティング

The ability to pattern proteins and other biomolecules onto substrates is important for capturing the spatial complexity of the extracellular environment. ...

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

交配との四分子の分離クラミドモナス遺伝子解析用

The unicellular green alga Chlamydomonas reinhardtii (Chlamydomonas) has become a popular organism for research in diverse areas of cell biology and ...

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs

ラット間葉系幹細胞（MSC）とMSCを派生シングルコロニーの分離の分離と濃縮

MSCs are a population of adult stem cells that is a promising source for therapeutic applications. These cells can be isolated from the bone marrow and ...

Crystallization of Membrane Proteins in Lipidic Mesophases

脂質中間相における膜タンパク質の結晶化

Membrane proteins perform critical functions in living cells related to signal transduction, transport and energy transformations, and, as such, are ...

Pull-down of Calmodulin-binding Proteins

プルダウンカルモジュリン結合タンパク質の

Calcium (Ca2+) is an ion vital in regulating cellular function through a variety of mechanisms. Much of Ca2+ signaling is mediated through the ...

Agarose Gel Electrophoresis for the Separation of DNA Fragments

DNA断片の分離のためのアガロースゲル電気泳動

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Agarose is isolated from ...

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

マウス胚性外胚葉顔と間葉の分離

 Orofacial clefts are the most frequent  craniofacial defects, which affect 1.5 in 1,000 newborns worldwide1,2. Orofacial clefting is caused by abnormal ...

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

STA-PUTの速度沈降を使用して造精細胞型の分離

Mammalian spermatogenesis is a complex  differentiation process that occurs in several stages in the seminiferous  tubules of the testes. Currently, there ...

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

IRE / IRP例：RNA  - タンパク質相互作用の研究のための電気泳動移動度シフトアッセイ（EMSA）

RNA/protein interactions are critical for post-transcriptional regulatory pathways. Among the best-characterized cytosolic RNA-binding proteins are iron ...