Name: electrophoretic separation of proteins
Uploaded: 2008-06-12T13:29:00
Description: Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

Bu deneysel bir yaklaşım için tam metin protokolü mevcuttur <a href="http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/mb1002a/current/abstract" target="_blank">Moleküler Biyoloji Mevcut Protokoller</a> .

electrophoretic separation of proteins

Elektroforez, proteinlerin (örneğin, hücre, hücre içi kesirler, sütun kesirler, veya immunoprecipitates) kompleks karışımlar ayrı altbirim kompozisyonları araştırmak için, protein örneklerinin homojenliği ve doğrulamak için kullanılır. Ayrıca başka uygulamalar için protein arındırmak için hizmet edebilir. Poliakrilamid jel elektroforezi, proteinler poliakrilamid jel matris gözenekler yoluyla bir elektrik alanı yanıt göç; gözenek büyüklüğü artan akrilamid konsantrasyonu azalır. Gözenek boyutu ve protein yükü, boyut ve şekil kombinasyonu protein göç oranı belirler. Bu ünitede, standart Laemmli yöntemi, sodyum dodesil sülfat (SDS) varlığı, denatüre edici koşullar altında yani süreksiz jel elektroforezi için açıklanmıştır.

Watch this Scientific Journal Video about Electrophoretic Separation of Proteins at JoVE.com

Electrophoretic Separation of Proteins

Bu video, poli-acrylimide jel elektroforezi (PAGE) kullanarak proteinlerin elektroforetik ayrımı için bir yöntem ortaya koymaktadır.

Proteinlerin Elektrogöçer Ayırma

Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to ...

Research

JoVE Journal

Biology

Staining Proteins in Gels

Jeller içinde Proteinler Boyama

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit describes protocols for ...

Microcontact Printing of Proteins for Cell Biology

Proteinlerin Hücre Biyolojisi Microcontact Baskı

The ability to pattern proteins and other biomolecules onto substrates is important for capturing the spatial complexity of the extracellular environment. ...

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

Çiftleşme ve tetrad Ayırma Chlamydomonas reinhardtii Genetik Analiz

The unicellular green alga Chlamydomonas reinhardtii (Chlamydomonas) has become a popular organism for research in diverse areas of cell biology and ...

Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs

Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs

İzolasyon ve Rat Mezenkimal Kök Hücreler zenginleştirilmesi (MKH) ve MKH Türetilmiş Tek koloni Ayırma

MSCs are a population of adult stem cells that is a promising source for therapeutic applications. These cells can be isolated from the bone marrow and ...

Crystallization of Membrane Proteins in Lipidic Mesophases

Lipidik Mesophases Membran Proteinleri Kristallendirilmesi

Membrane proteins perform critical functions in living cells related to signal transduction, transport and energy transformations, and, as such, are ...

Pull-down of Calmodulin-binding Proteins

Kalmodulin bağlayıcı Proteinler, Pull-down

Calcium (Ca2+) is an ion vital in regulating cellular function through a variety of mechanisms. Much of Ca2+ signaling is mediated through the ...

Agarose Gel Electrophoresis for the Separation of DNA Fragments

DNA kırıntıları Ayrılma Agaroz Jel Elektroforezi

Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Agarose is isolated from ...

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Fare Embriyonik Yüz Ektoderm ve mesenchyme ayrılması

 Orofacial clefts are the most frequent  craniofacial defects, which affect 1.5 in 1,000 newborns worldwide1,2. Orofacial clefting is caused by abnormal ...

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

STA-PUT Hız Sedimentasyon kullanma spermatogenic Hücre Tiplerinin Ayrılması

Mammalian spermatogenesis is a complex  differentiation process that occurs in several stages in the seminiferous  tubules of the testes. Currently, there ...

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Electrophoretic Mobility Shift Assay EMSA for the Study of RNA-Protein Interactions: The IRE/IRP Example

IRE / IRP Örnek: RNA-protein Etkileşimleri Çalışmaları elektroforetik hareketlilik Shift Testi (EMSA)

RNA/protein interactions are critical for post-transcriptional regulatory pathways. Among the best-characterized cytosolic RNA-binding proteins are iron ...