Name: electroporation of mycobacteria
Uploaded: 2008-05-23T14:35:00
Duration: 11 min 57 s
Description: Watch this Scientific Journal Video about Electroporation of Mycobacteria at JoVE.com

The authors have nothing to disclose.

electroporation of mycobacteria

高效率的转变，是一个主要的限制，在分枝杆菌的研究。属分枝杆菌可以难以转变;这主要是由于厚糯细胞壁，但又因较远相关的物种，如大肠杆菌和枯草芽孢杆菌，大多数分子生物学技术已开发。尽管存在这些障碍，分枝杆菌质粒已确定，现在已经被描述的许多分支杆菌物种的DNA转化。引入分枝杆菌DNA的最成功的方法是电。许多参数作出贡献的成功转型，这包括物种/应变，转化的DNA的性质，所使用的选择标记，生长介质，并为电脉冲的条件。这里有详细的优化方法改造的缓慢和快速种植者。据报道，为不同的分枝杆菌物种和各种可选的标记的转化效率。

Watch this Scientific Journal Video about Electroporation of Mycobacteria at JoVE.com

Electroporation of Mycobacteria

结核分枝杆菌的致病性战略仍然知之甚少。品种最缓慢的增长速度，细胞壁的坚不可摧的性质，和病原体的危害，使分枝杆菌的研究和主要负责为我们这些生物体缺乏了解。在这个视频中，我们将演示电的技术，它涉及到一个简短的电脉冲，让遭受细胞DNA的条目。它是最广泛使用的方法引入分枝杆菌细胞的DNA。

电穿孔分枝杆菌

High efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly ...

Research

JoVE Journal

Biology

In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos

E16大鼠胚胎在子宫内侧脑室注射和电穿孔

In-utero in-vivo injection and electroporation of the embryonic rat neocortex provides a powerful tool for the manipulation of individual progenitors ...

科研

生物学

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

E15小鼠胚胎在子宫内侧脑室注射和电穿孔

In-utero in-vivo injection and electroporation of the embryonic mouse neocortex provides a powerful tool for the manipulation of individual progenitors ...

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

使用哺乳动物骨骼肌的DNA转染在体内电穿孔

 A growing interest in cell biology is to express transgenically modified forms of essential proteins (e.g. fluorescently tagged constructs and/or mutant ...

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti. 

原代培养及质粒电穿孔尔蒂小鼠器官。

In all mammals, the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea of the ...

Electroporation of Craniofacial Mesenchyme

颅面间质细胞的电

Electroporation is an efficient method of delivering DNA and other charged macromolecules into tissues at precise time points and in precise locations. ...

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

在体内电穿孔Morpholinos到成年斑马鱼视网膜

Many devastating inherited eye diseases result in progressive and irreversible blindness because humans cannot regenerate dying or diseased retinal ...

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

再生的成年斑马鱼尾鳍在吗啉代入体内电穿孔

Certain species of urodeles and teleost fish can regenerate their tissues. Zebrafish have become a widely used model to study the spontaneous regeneration ...

In Ovo Electroporation in Embryonic Chick Retina

In Ovo Electroporation in Embryonic Chick Retina

在OVO在鸡胚视网膜电穿孔

Chicken embryonic retina is an excellent tool to study retinal development in higher vertebrates. Because of large size and external development, it is ...

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

基因转移到老年人的鸡胚<em&gt;前OVO</em&gt;电穿孔

The chicken embryo provides an  excellent model system for studying gene function and regulation during  embryonic development. In ovo electroporation is ...

In Vivo Microinjection and Electroporation of Mouse Testis

In Vivo Microinjection and Electroporation of Mouse Testis

在小鼠睾丸活体显微注射和电穿孔

This video and article contribution gives a comprehensive description of microinjection and electroporation of mouse testis in vivo. This particular ...