Name: mouse epidermal neural crest stem cell (epi-ncsc) cultures
Uploaded: 2008-05-09T01:00:00
Description: Watch this Scientific Journal Video about Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures at JoVE.com

技术支持：USPHS授予NS38500，国家神经疾病研究所和中风，美国国立卫生研究院，美国威斯康星州东南的生物医学技术联盟，Milwauke，美国威斯康星州，密尔沃基雄鹿队拜伦Riesch瘫痪基金会，美国威斯康星州;鹰的兄弟订购，中西部章美国国立脊髓损伤协会，美国密尔沃基章，东北英格兰干细胞研究所，英国纽卡斯尔大学，英国。

从成年鼠晶须卵泡凸出的剖析<ol><li>我们使用10个星期到6个月大的老鼠。年轻的老鼠经常会产生更多的细胞。鼠标和淹没整个动物安乐死成1:1的优碘（碘伏溶液）和过氧化氢，约3分钟（从药房）的混合物。 </li><li>水枪整个鼠标，特别是面部的地区，用75％乙醇，并进行鼠标在层流罩夹层显微镜。 </li><li>解剖晶须垫，小心地避免切成头发灯泡的HBSS池。 </li><li>在室温下的HBSS解剖胡须毛囊和游?...

<ol>
	<li>Sieber-Blum, M., Grim, M., YF, H. u., Szeder, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pluripotent+neural+crest+stem+cells+in+the+adult+hair+follicle.">Pluripotent neural crest stem cells in the adult hair follicle.</a> Dev. Dyn. 231, 258-269 (2004).</li><li>Sieber-Blum, M., Grim, M., Tuan, R., Grim, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+adult+hair+follicle+-+cradle+for+pluripotent+neural+crest+stem+cells.">The adult hair follicle - cradle for pluripotent neural crest stem cells.</a> Birth Defects Research (Part C). 72, 162-172 (2004).</li><li>Sieber-Blum, M., Schnell, L., Grim, M., Schneider, R., Schwab, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+epidermal+neural+crest+stem+cell+(EPI-NCSC)+grafts+in+the+lesioned+spinal+cord.">Characterization of epidermal neural crest stem cell (EPI-NCSC) grafts in the lesioned spinal cord.</a> Pub. 32, 67-81 (2006).</li><li>Hu, Y. F., Zhang, Z. -. J., Sieber-Blum, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+epidermal+neural+crest+stem+cell+(EPI-NCSC)+molecular+signature.">An epidermal neural crest stem cell (EPI-NCSC) molecular signature.</a> Stem Cells. 24, 2692-2702 (2006).</li></ol>

凭借其迁徙的能力，可以分离出高纯度的干细胞可以在体外培养扩增人口EPI的非公务员合约。作为在一个成人的位置胚胎残余，EPI -非公务员合约是为未来的细胞替代疗法，生物医学工程和/或再生医学的潜在吸引力的候选人。我们已经测试了EPI的非公务员合约雇员在脊髓损伤小鼠模型，它们显示的地方的理想特性。通过LongSAGE（www.ncbi.nlm.nih.gov / GEO，序列号GSE4680）的基因表达图谱，我们表明，正如所料，胚胎神?...

mouse epidermal neural crest stem cell (epi-ncsc) cultures

EPI的非公务员合约雇员是在成人的位置，毛囊隆起的胚胎神经嵴的残余。他们是多能干细胞具有产生分化的细胞类型广泛，包括神经元，神经细胞，平滑肌细胞，骨/软骨细胞和黑色素细胞的生理属性。 EPI的非公务员合约雇员在毛茸茸的皮肤很容易访问的，并可以作为分离出高纯度的干细胞人口。此影片提供了一个详细的协议，准备从鼠标EPI的非公务员合约雇员的文化胡须毛囊。成年小鼠触须垫被删除，和胡须毛囊解剖。卵泡然后切横纵向和随后的上方和下方的凸起区域。凸起是从胶原蛋白胶囊，并在培养板中。 EPI的非公务员合约雇员3至4天后开始隆起植移民。

Watch this Scientific Journal Video about Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures at JoVE.com

Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures

在这里，我们显示我们的方法分离出小鼠表皮神经嵴干细胞（EPI的非公务员合约雇员）。技术涉及微解剖胡须毛囊，孤立的隆起和placeing组织培养。 EPI的非公务员合约开始从到底层的隆起植移民，在3 - 4天。

小鼠表皮神经嵴干细胞（EPI的非公务员合约雇员）文化

EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the ...

Research

JoVE Journal

Biology

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Culture of Mouse Neural Stem Cell Precursors

培养小鼠神经干细胞的前体

Primary neural stem cell cultures are useful for studying the mechanisms underlying central nervous system development. Stem cell research will increase ...

科研

生物学

Dissection of 6.5 dpc Mouse Embryos

6.5 DPC小鼠胚胎剖析

Analysis of gene expression patterns during early stages of mammalian embryonic development can provide important clues about gene function, cell-cell ...

Counting Human Neural Stem Cells

人类神经干细胞计数

Knowledge of the exact number of viable cells in a given volume of a cell suspension is required for many routine tissue culture manipulations, such as ...

Immunocytochemistry: Human Neural Stem Cells

免疫组化：人类神经干细胞

Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of ...

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

使用琼脂糖凝胶为基础的微流体装置的评估神经干细胞的运动

While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is ...

Generation of Single-Cell Suspensions from Mouse Neural Tissue

从小鼠神经组织产生单细胞悬浮液

Within the nervous system, hundreds of neuronal and glial cell types have been described. Each specific cell type in the brain or spinal cord has a ...

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

Preparation of Pooled Human Platelet Lysate pHPL as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures

汇集人类血小板裂解液作为一种有效的补充（pHPL）的动物血清自由人干细胞培养制备

Platelet derived growth factors have been shown to stimulate cell proliferation efficiently in vivo1,2 and in vitro. This effect has been reported for ...

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

在斑马鱼胚胎的单个神经元和神经嵴细胞的细胞运动和肌动蛋白细胞骨架的实时成像

The zebrafish is an ideal model for imaging cell behaviors during development in vivo. Zebrafish embryos are externally fertilized and thus easily ...

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

快速高密度的人类胚胎干细胞培养成纤维细胞去除

Mouse embryonic fibroblasts (MEFs) were used to establish human embryonic stem cells (hESCs) cultures after blastocyst isolation1. This feeder system ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

诱导多能干细胞来源于小鼠的生成

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...