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Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

DOI :

10.3791/772-v

May 9th, 2008

May 9th, 2008

11,369 Views

1Institute of Human Genetics and Northeast England Stem Cell Institute, Newcastle University, 2Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin

Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.

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Mouse Epidermal Neural Crest Stem Cell

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