Name: generation of bone marrow derived murine dendritic cells for use in 2-photon imaging
Uploaded: 2008-07-09T16:32:00
Description: Watch this Scientific Journal Video about Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging at JoVE.com

健康Kirchsteinフェローシップ博士号を取得する前のフェローシップAI - 64128（MPM）、GM - 41514（MDC）、GM - 48071（IP）の国立研究所

1）1つのマウスから、両方の大腿骨の骨を削除します。 <ol><li>筋肉を切り取ると、関節の上下の大腿骨（膝と腰を）公開する解剖鋏を使用してください。解剖ピンセットで大腿骨の中央をつかみ、可能な限りそのまま骨端を残すために、上記と関節の下にカット。 </li><li>小さな解剖鋏を使用して、できるだけ多くの筋肉のように拭き取ります。 RPMIの皿に大腿骨を移す。 </li><li>全ての手順?...

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	<li>Miller, M. J., Safrina, O., Parker, I., Cahalan, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+the+single+cell+dynamics+of+CD4++T+cell+activation+by+dendritic+cells+in+lymph+nodes.">Imaging the single cell dynamics of CD4+ T cell activation by dendritic cells in lymph nodes.</a> J Exp Med. 200, 847-856 (2004).</li><li>Henrickson, S. 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樹状細胞は適応免疫応答と日付に特徴づけられる最も効率的な抗原提示細胞の主要なメディエーターである。文学におけるヒトとマウスの樹状細胞培養のための方法は異なる樹状細胞型の開発に影響を与えるために使用されるサイトカインの種類が異なります。特に、GM - CSF、flt3L、IL4、IL13、TNF -αとIFN -γは、成熟未成熟、炎症性および定常状態の試験管3-7に樹状細胞由来のマウス骨髄のように生成?...

generation of bone marrow derived murine dendritic cells for use in 2-photon imaging

マウス樹状細胞の調製のためのいくつかの方法が文献で見つけることができます。ここで、我々は文化の中では85％以上のCD11c高樹状細胞を生成する手法を提案することを皮下注射し、抗原特異的T細胞（ビデオを参照）に存在する抗原を後に流入領域リンパ節へのホーム。さらに、我々は、10日目に、培養細胞の形態は、成熟樹状細胞の典型的であり、細胞の&lt;85％がCD11chighである、樹状細胞の成熟を、追跡するためにエッセン楽器Incucyteを使用してください。 2光子イメージングによる末梢リンパ節における抗原提示の研究では、樹状細胞とT細胞の相互作用1、2の3つのフェーズが存在することを明らかにした。フェーズIは、樹状細胞1、2を運ぶ非常に運動性の抗原特異的T細胞と抗原との間の短いシリアル接点で構成されています。第二段階は、樹状細胞1、2をもつ抗原特異的T細胞と抗原との間の長期にわたる接触によってマークされます。最後に、第III相は、運動性を回復し、1、2を分割し始めて、樹状細胞から切り離すT細胞によって特徴付けられる。これは、抗原負荷細胞トラッカー色素標識樹状細胞の二光子励起イメージングによって解析することができる抗原特異的相互作用のタイプの一例です。

Watch this Scientific Journal Video about Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging at JoVE.com

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

樹状細胞による二次リンパ器官における抗原提示がT細胞媒介免疫応答の開始に重要です。ここでは、マウス樹状細胞を骨髄由来の文化、活性化、および2光子イメージングのための標識を示す。

2光子イメージングで使用するための骨髄由来マウス樹状細胞の世代

Several methods for the preparation of murine dendritic cells can be found in the literature. Here, we present a method that produces greater than 85% ...

Research

JoVE Journal

Biology

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

解剖とマウスの末梢リンパ節の2 - フォトンイメージング

Two-photon imaging has revealed an elegant choreography of motility and cellular interactions within the lymph node under basal conditions and at the ...

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

2光子顕微鏡を用いたマウスの胸腺のその場イメージングで

Two-photon Microscopy (TPM) enables us to image deep into the thymus and document the events that are important for thymocyte development.  To follow the ...

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

頭蓋窓を介して新皮質の血流の2光子イメージングのための方法

The ability to image the cerebral vasculature (from large vessels to capillaries) and record blood flow dynamics in the intact brain of living rodents is ...

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice

レイヤーin vivoでの2光子カルシウムイメージングマウスの2 / 3

To understand network dynamics of microcircuits in the neocortex, it is essential to simultaneously record the activity of a large number of neurons .  ...

Culture of myeloid dendritic cells from bone marrow precursors

骨髄前駆細胞から骨髄樹状細胞の培養

Myeloid dendritic cells (DCs) are frequently used to study the interactions between innate and adaptive immune mechanisms and the early response to ...

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

エレクトロポレーションのためのマウス骨髄からの一次造血細胞培養の準備

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser ...

Homing of Hematopoietic Cells to the Bone Marrow

骨髄の造血細胞のホーミング

Homing is the phenomenon whereby transplanted hematopoietic cells are able to travel to and engraft or establish residence in the bone marrow.  Various ...

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

成体マウス頭蓋冠骨髄中の造血幹細胞および前駆細胞のin vivo 4次元トラッキングで

Through a delicate balance between quiescence and proliferation, self renewal and production of differentiated progeny, hematopoietic stem cells (HSCs) ...

Osteoclast Derivation from Mouse Bone Marrow

マウス骨髄からの破骨細胞の導出

Osteoclasts are highly specialized cells that are derived from the monocyte/macrophage lineage of the bone marrow. Their unique ability to resorb both the ...

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

マウス骨髄由来の樹状細胞とマクロファージの蛍光融合タンパク質の発現

Dendritic cells and macrophages are crucial cells that form the first line of defense against pathogens. They also play important roles in the initiation ...