Name: retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus
Uploaded: 2008-06-17T17:19:00
Description: Watch this Scientific Journal Video about Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus at JoVE.com

作者想感谢美国卫生署助理基金会从全国青光眼计划的支持。

<ol><li>麻醉大鼠腹腔注射氯胺酮（80mg/kg）和甲苯噻嗪（8mg/kg）（体积比为2:1）。应用无菌眼润滑剂油膏，以防止在手术过程中角膜干燥。 </li><li>剃的头皮毛（从眼睛到耳朵齐平）和固定头头部头部舞台上钳，并设置其门牙，门齿栏。 </li><li>消毒其次70％的酒精与10％的碘伏解决方案的操作面积。 </li><li>切开皮肤和肌肉，暴露颅骨和缝合线（矢状面，冠状和横向缝线）（图1）。作为一个切口面积飞溅（不超过2毫克/...

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	<li>Ahmed, A. K. M. F., Dong, K., Sugioka, K., Yamadori, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retrograde+double+labeling+of+retinal+ganglion+cells+in+the+newborn+and+adult+albino+rat.">Retrograde double labeling of retinal ganglion cells in the newborn and adult albino rat.</a> Neurosci. Protocols. 1, 1-11 (1996).</li><li>Chan, H. C., Chang, R. C. C., Ip, A. K. C., Chiu, K., Yuen, W. H., Zee, S. Y., So, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroprotective+effects+of+Lycium+barbarum+Lynn+on+protecting+retinal+ganglion+cells+in+an+ocular+hypertension+model+of+glaucoma.">Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma.</a> Experimental Neurology. 203, 269-273 (2007).</li><li>Forrester, J., Peters, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nerve+fibers+in+the+optic+nerve+of+rat.">Nerve fibers in the optic nerve of rat.</a> Nature. 214, 245-247 (1967).</li><li>Ji, J. Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. W., Hugon, J., So, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CNTF+promotes+survival+of+retinal+ganglion+cells+after+induction+of+ocular+hypertension+in+rats:+the+possible+involvement+of+STAT3+pathway.">CNTF promotes survival of retinal ganglion cells after induction of ocular hypertension in rats: the possible involvement of STAT3 pathway.</a> European Journal of Neuroscience. 19, 265-272 (2004).</li><li>Li, R. S., Chen, B. Y., Tay, D. K., Chan, H. H. L., Pu, M. L., So, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Melanopsin-Expressing+Retinal+Ganglion+Cells+Are+More+Injury-Resistant+in+a+Chronic+Ocular+Hypertension+Model.+Investigative.">Melanopsin-Expressing Retinal Ganglion Cells Are More Injury-Resistant in a Chronic Ocular Hypertension Model. Investigative.</a> Ophthalmology &amp; Visual Science. 47, 2951-2958 (2006).</li></ol>

<ol><li>为什么要使用这种手术方法对视网膜神经节细胞计数？ 在大鼠视网膜神经节细胞中，约98％（研资局）是众所周知的对侧上丘（SC）的项目。 SC的表面上应用氟金标签几乎所有的视网膜神经节细胞，使我们能够在这个最脆弱的视网膜神经集中在青光眼。切断视神经断端2天FG逆行标记与研资局的比较，研资局生存的干扰降至最低。比较甲酚紫染色法的无长​​突细胞的污渍，研资局与血管内皮细胞在视...

retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus

视网膜神经节细胞计数（研资局）是必不可少的评估，尤其是在青光眼视网膜变性。可靠的研资局的标签，是为任何治疗效果评估的基本。在大鼠的视网膜神经节细胞中，约98％是已知的项目对侧上丘（SC）（Forrester和彼得斯，1967年）。 SC的表面上应用氟金（FG）的标签，几乎所有的视网膜神经节细胞，使我们能够在这个最脆弱的视网膜神经集中在青光眼。 FG是采取了由视网膜神经节细胞的轴突终端和双边运输逆行其胞体在视网膜上。切断视神经断端2天FG逆行标记与研资局的比较，研资局生存的干扰降至最低。甲酚紫染色比较污渍的视网膜神经节细胞，无长突细胞在视网膜神经节细胞层血管的内皮细胞，这种标记方法是更具体的研资局。本视频介绍了SC的表面FG申请研资局逆行标记的方法。手术过程包括钻颅骨;吸皮质揭露SC和应用在整个SC的背水面明胶海绵显示。已获得有用的技巧，避免大量的颅​​内出血和SC的愿望。

Watch this Scientific Journal Video about Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus at JoVE.com

Retrograde Labeling of Retinal Ganglion Cells by Application of Fluoro-Gold on the Surface of Superior Colliculus

该视频描述了应用表面上丘（SC）的氟金（FG）逆行标记研资局的方法。技术涉及的颅骨钻，吸皮层，整个SC背水面和应用明胶海绵。

上丘表面上应用氟金逆行标记视网膜神经节细胞

Retinal ganglion cell (RGC) counting is essential to evaluate retinal degeneration especially in glaucoma. Reliable RGC labeling is fundamental for ...

Research

JoVE Journal

Biology

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

在子宫内和前体内的基因表达在小鼠视网膜神经节细胞的电穿孔

The retina and its sole output neuron, the retinal ganglion cell (RGC), comprise an excellent model in which to examine biological questions such as cell ...

科研

生物学

Fluorescent Labeling of Drosophila Heart Structures

Fluorescent Labeling of Drosophila Heart Structures

荧光标记的果蝇心结构

The Drosophila melanogaster dorsal vessel, or heart, is a tubular structure comprised of a single layer of contractile cardiomyocytes, pericardial cells ...

Isolation of Retinal Stem Cells from the Mouse Eye

从鼠眼视网膜干细胞的分离

The adult mouse retinal stem cell (RSC) is a rare quiescent cell found within the ciliary epithelium (CE) of the mammalian eye1,2,3. The CE is made up of ...

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

为极化上皮细胞注射质粒的小GTP酶的功能分析

Epithelial cells polarize their plasma membrane into biochemically and functionally distinct apical and basolateral domains where the apical domain faces ...

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase COX/SDH Double-labeling Histochemistry

使用细胞色素线粒体呼吸功能的可视化 C氧化酶/琥珀酸脱氢酶（COX / SDH），双标组织化学

Mitochondrial DNA (mtDNA) defects are an important cause of disease and may underlie aging and aging-related alterations 1,2. The mitochondrial theory of ...

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Avidity-based Extracellular Interaction Screening AVEXIS for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

基于亲和力，外的相互作用筛选（AVEXIS）外低亲和力受体 - 配体相互作用的可扩展检测

Extracellular protein:protein interactions between secreted or membrane-tethered proteins are critical for both initiating intercellular communication and ...

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

利用单克隆抗体TG30和GCTM-2检测细胞表面抗原，促进人类胚胎干细胞的浓缩和清除

Human embryonic stem cells (hESC) can self-renew indefinitely in vitro, and with the appropriate cues can be induced to differentiate into potentially all ...

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

Through a delicate balance between quiescence and proliferation, self renewal and production of differentiated progeny, hematopoietic stem cells (HSCs) ...

A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

一个标准化的方法肝窦内皮细胞的分析及其窗孔用扫描电子显微镜

Liver sinusoidal endothelial cells are the gateway to the liver, their transcellular fenestrations allow the unimpeded transfer of small and dissolved ...

Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP

具体的基因组区域及相关分子鉴定enChIP隔离

The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the ...