Name: in vitro differentiation of mouse embryonic stem (mes) cells using the hanging drop method
Uploaded: 2008-07-23T22:33:00
Description: Watch this Scientific Journal Video about In vitro Differentiation of Mouse Embryonic Stem mES Cells Using the Hanging Drop Method at JoVE.com

<ol><li>涂胶T75烧瓶中，在37℃，5％的<sub>CO</sub> 2组织培养箱培养过夜之一的前一天使用0.1％的明胶溶液和孵化板。 </li><li>取出孵化mES细​​胞，吸出培养基，用PBS冲洗胚胎干细胞培养，加入0.05％胰蛋白酶溶液大衣的菜（2 ml/100mm板）底部。 </li><li>孵育在37 ° C约1分钟，待细胞塌板。 <br /></li><li>轻轻磨碎（吸管向上和向下）的4至6倍，以分散与巴斯德吸管插入的ES细胞的胰酶消化细胞，。分散的ES细胞转移到15毫升锥?...

<ol>
	<li>Wobus, A. M., Wallukat, G., Hescheler, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pluripotent+mouse+embryonic+stem+cells+are+able+to+differentiate+into+cardiomyocytes+expressing+chronotropic+responses+to+adrenergic+and+cholinergic+agents+and+Ca2++channel+blockers.">Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers.</a> <em style='font-style: italic'>Differentiation</em>. <b>48</b>, 173-182 (1991).</li><li>Metzger, J. M., Lin, W. I., Samuelson, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transition+in+cardiac+contractile+sensitivity+to+calcium+during+the+in+vitro+differentiation+of+mouse+embryonic+stem+cells.">Transition in cardiac contractile sensitivity to calcium during the in vitro differentiation of mouse embryonic stem cells.</a> <em style='font-style: italic'>J Cell Biol</em>. <b>126</b>, (1994).</li></ol>

<p>悬滴法的缺点如下：不超过50ul，由于维持表面张力的盖子上悬滴一滴的液体的体积是有限的，它不可能改变中期挂滴。形成胚滴直接用显微镜观察也很难在种植。更进一步，悬滴法是由两个步骤组成，因此，悬滴法的一系列步骤可能会很麻烦。 </p>

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in vitro differentiation of mouse embryonic stem (mes) cells using the hanging drop method

<p class="jove_content">干细胞具有显着的潜力发展成许多不同的细胞类型。当一个干细胞分裂时，每个新细胞的潜力仍然是一个干细胞，或成为另一个类型的细胞与一个更专业的功能，这是有前途的科学顶尖科学家研究细胞疗法来治疗疾病的可能性。当没有暂停antidif​​ferentiation因素文化时，胚胎干细胞自发分化和形成立体的多细胞聚集。这些细胞聚集被称为胚体（EB）。悬滴文化是一种广泛使用的EB形成诱导方法。悬滴的圆形底部，使胚胎干细胞，它可以提供一个良好的环境，形成EBS mES细胞的聚集。在悬滴aggregatied的ES细胞的数量是可以控制的不同细胞的数量在最初的细胞悬液，将挂在培养皿的盖子下降。使用这种方法，我们可以再现的形式从同质分化的ES细胞的预定人数。</p>

Watch this Scientific Journal Video about In vitro Differentiation of Mouse Embryonic Stem mES Cells Using the Hanging Drop Method at JoVE.com

In vitro Differentiation of Mouse Embryonic Stem mES Cells Using the Hanging Drop Method

<p class="jove_content">本视频演示了如何在小鼠胚胎干细胞体外分化胚体采用悬滴法进行。</p>

在体外分化的小鼠胚胎干细胞（MES）使用悬滴法

Stem cells have the remarkable potential to develop into many different cell types. When a stem cell divides, each new cell has the potential to either ...

Research

JoVE Journal

Biology

In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

图案的使用生物倒装芯片的胚胎干细胞

Cell-cell interactions consisting of diffusible signaling and cell-cell contact (juxtacrine signaling) are important in numerous biological processes such ...

科研

生物学

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells

在体外和体内生物发光记者人类胚胎干细胞的基因显像

The discovery of human embryonic stem cells (hESCs) has dramatically increased the tools available to medical scientists interested in regenerative ...

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

在人类胚胎干细胞体外培养的标签用于磁共振成像

Human embryonic stem cells (hESC) have demonstrated the ability to restore the injured myocardium. Magnetic resonance imaging (MRI) has emerged as one of ...

The Preparation of <em>Drosophila</em> Embryos for Live-Imaging Using the Hanging Drop Protocol

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

编制<em>果蝇</em>实时成像的胚胎使用悬滴协议

Green fluorescent protein (GFP)-based timelapse live-imaging is a powerful technique for studying the genetic regulation of dynamic processes such as ...

Isolation and Derivation of Mouse Embryonic Germinal Cells

小鼠胚胎生殖细胞的分离和推导

The ability of embryonic germinal cells (EG) to differentiate into primordial germinal cells (PGCs) and later into gametes during early developmental ...

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

胚胎干细胞分化成少突胶质前体

Oligodendrocytes are the myelinating cells of the central nervous system. For regenerative cell therapy in demyelinating diseases, there is significant ...

Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons

高效的小鼠胚胎干细胞分化成运动神经元

Direct differentiation of embryonic stem (ES) cells into functional  motor neurons represents a promising resource to study disease mechanisms, to  screen ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

诱导多能干细胞来源于小鼠的生成

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells <em>In vitro</em>

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

新生小鼠皮肤干细胞分化成生殖细胞<em在体外</em

Studying  germ cell formation and differentiation has traditionally been very difficult  due to low cell numbers and their location deep within developing ...

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

分析单个人类胚胎干细胞通过定量RT-PCR

Heterogeneity of stem cell population hampers detailed understanding of stem cell biology, such as their differentiation propensity toward different ...