Name: a rapid technique for the visualization of live immobilized yeast cells
Uploaded: 2006-11-09T00:00:00
Description: Watch this Scientific Journal Video about A Rapid Technique for the Visualization of Live Immobilized Yeast Cells at JoVE.com

我们要感谢他们的帮助皮特休斯敦和尤金妮亚徐。

<ol><li>时间当然epifluorescence显微镜固定化细胞。请注意，这个协议是唯一有用的，如果您的显微镜物镜低于显微镜阶段。 </li><li>在液体介质中的浓度所需的位置细胞长盖玻片（大约24x50mm） </li><li>切割的一种人工合成的介质板（这些具有较低的自体荧光）和地点在细胞所需的大小的琼脂块。广场一侧的镊子处理与细胞接触的琼脂块。 </li><li>对于较短时间的课程（最多3个小时），这个成立...

<ol>
	<li>Xu, E. Y., Zawadzki, K. A., Broach, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-cell+observations+reveal+intermediate+transcriptional+silencing+states.">Single-cell observations reveal intermediate transcriptional silencing states.</a> Mol. Cell. 23, 219-229 (2006).</li></ol>

这是一种快速的技术，无法移动，酵母细胞中，而仍然允许增长。我们遵循长达十几个小时的酵母生长，与酵母在整个实验过程中显示野生型分工率。请注意，此安装需要的物镜显微镜下面的阶段。

a rapid technique for the visualization of live immobilized yeast cells

我们在座的一个epifluorescence显微镜日益增长的酵母细胞固定化和可视化的简单，快速，和极其灵活的技术。该技术同样适用于静态的酵母种群，或时间制课程实验达10个小时的长度可视化。我的显微镜调查在沉默交配基因在酿酒酵母中的后生继承。有两个位点的沉默交配，HML和HMR的，通常不会表达，因为它们是在异包装的。在sir1突变的背景沉默减弱等，每个座位可以表达或沉默的后生状态，所以在人口作为一个整体有一个HML和HMR的后生国家不同的细胞混合。我的显微镜证明在单个细胞之间的HML和HMR的后生状态，没有关系。 sir1细胞随机开关后生国家，建立在先前表示的轨迹沉默或表达了先前沉默的轨迹。我的时间当然显微镜跟踪个人sir1细胞和他们的后代将比分四种可能的后生开关频率，因此每个sir1细胞后生国家的稳定。也许等。分子。细胞2006年。

Watch this Scientific Journal Video about A Rapid Technique for the Visualization of Live Immobilized Yeast Cells at JoVE.com

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

一个快速增长的固定化酵母细胞的可视化技术，在这里沉默交配位点HML和HMR的荧光灯记者

一个现场的固定化酵母细胞可视化的快速技术

We present here a simple, rapid, and extremely flexible technique for the immobilization and visualization of growing yeast cells by epifluorescence ...

Research

JoVE Journal

Biology

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

裂殖酵母的活细胞定量荧光显微镜分析

Several microscopy techniques are available today that can detect a specific protein within the cell. During the last decade live cell imaging using ...

科研

生物学

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

在哺乳动物细胞中的内质网定位的mRNA的可视化

In eukaryotes, most of the messenger RNAs (mRNAs) that encode secreted and membrane proteins are localized to the surface of the endoplasmic reticulum ...

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

番茄/ GFP-FLP / FRT方法马赛克成人的实时成像<em&gt;果蝇</em&gt;感光细胞

The Drosophila eye is widely used as a model  for studies of development and neuronal degeneration. With the powerful mitotic  recombination technique, ...

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

检测现场的<em&gt;大肠杆菌</em&gt; O157：H7细胞的PMA-qPCR的

A unique open reading frame (ORF) Z3276 was identified as a specific genetic marker for E. coli O157:H7. A qPCR assay was developed for detection of E. ...

Visualization of G3BP Stress Granules Dynamics in Live Primary Cells

中G3BP应力颗粒动力学在Live原代细胞可视化

SGs can be visualized in cells by immunostaining of specific protein components or polyA+ mRNAs. SGs are highly dynamic and the study of their assembly ...

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移

Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live ...

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

蛋白质纯化技术，可以检测SUMO化修饰和芽殖酵母蛋白动粒和Ndc10泛素Ndc80的

Post-translational Modifications (PTMs), such as phosphorylation, methylation, acetylation, ubiquitination, and sumoylation, regulate the cellular ...

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

肾毒素显微注射斑马鱼到模型急性肾损伤

The kidneys are susceptible to harm from exposure to chemicals they filter from the bloodstream. This can lead to organ injury associated with a rapid ...

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

使用微流体装置来衡量寿命和细胞表型单芽殖酵母细胞

Budding yeast Saccharomyces cerevisiae is an important model organism in aging research. Genetic studies have revealed many genes with conserved effects ...

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

细菌、酵母和人类细胞中瞬态形成的分子茶龙组件的原位监测

J-domain proteins (JDPs) form the largest and the most diverse co-chaperone family in eukaryotic cells. Recent findings show that specific members of the ...