JoVE Logo
Faculty Resource Center
Authors
Librarians
High Schools
About

Sign In

Human ES cells: Starting Culture from Frozen Cells

DOI :

10.3791/86-v

•

November 9th, 2006

November 9th, 2006

•
11,980 Views
1Department of Molecular and Cell Biology, Harvard

Here we demonstrate how our lab begins a HuES human embryonic stem cell line culture from a frozen stock.

Tags

Human ES Cells
Culture
Frozen Cells
Embryonic Stem Cell Line
HuES Media
Mouse Embryonic Fibroblast Feeder Cells
Serum
Cell Debris
Liquid Nitrogen Storage
80C Freezer
Thawing
Freezing Media
DMSO
Differentiation
Suspension
Seeding
MEF Plate
Incubator
Media Replacement

-- Views

Related Videos

JoVE Logo

Privacy

Terms of Use

Policies

Contact Us

Recommend to library

JoVE NEWSLETTERS

Research

Education

Authors

Librarians

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved