Name: survivable stereotaxic surgery in rodents
Uploaded: 2008-10-06T00:00:00
Description: Watch this Scientific Journal Video about Survivable Stereotaxic Surgery in Rodents at JoVE.com

支持DK065872（ENP），一个卓越的史密斯一家基金会在生物医学研究奖（ENP），F31 DA023760。

摘要两个月的平均年龄C57BL/6J小鼠或同等或3个月岁，平均年龄SD大鼠或同等学历麻醉与氯胺酮（60毫克/公斤大鼠的IP; 100毫克/公斤对小鼠的IP）和甲苯噻嗪（10毫克/公斤，对于任何一个物种的IP）。镇静是监测使用一个温柔的脚趾捏撤回反射<a href="/index/details.stp?ID=236">证明Walantus等</a> 。（朱庇特，6，2007年） <a href="/index/details.stp?ID=404">和Szot</a>等（朱庇特，9日，2007） 。体温可提供通过thermostatre...

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	<li>Bungay, P. M., Newton-Vinson, P., Isele, W., Garris, P. A., Justice, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microdialysis+of+dopamine+interpreted+with+quantitative+model+incorporating+probe+implantation+trauma.">Microdialysis of dopamine interpreted with quantitative model incorporating probe implantation trauma.</a> J. Neurochem. 86, 932-946 (2003).</li><li>Chen, K. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+tissue+trauma+on+the+characteristics+of+microdialysis+zero-net-flux+method+sampling+neurotransmitters.">Effects of tissue trauma on the characteristics of microdialysis zero-net-flux method sampling neurotransmitters.</a> Journal of Theor. Biology. 238, 863-881 (2006).</li><li>Geiger, B. M., Behr, G. G., Frank, L., Caldera-Siu, A. D., Beinfeld, M. C., Kokkotou, E. G., Pothos, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evidence+for+defective+mesolimbic+dopamine+exocytosis+in+obesity-prone+rats.">Evidence for defective mesolimbic dopamine exocytosis in obesity-prone rats.</a> FASEB Journal. 8, 2740-2746 (2008).</li><li>Pothos, E. N., Creese, I., Hoebel, B. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Restricted+eating+with+weight+loss+selectively+decreases+extracellular+dopamine+in+the+nucleus+accumbens+and+alters+dopamine+response+to+amphetamine,+morphine+and+food+intake.">Restricted eating with weight loss selectively decreases extracellular dopamine in the nucleus accumbens and alters dopamine response to amphetamine, morphine and food intake.</a> The Journal of Neuroscience. 15, 6640-6650 (1995).</li></ol>

在体内微透析技术的首选工具，衡量一个活生生的动物不同脑网站的多个神经递质及代谢产物。但是，它只监视外的神经化学物质的水平，并没有提供时间分辨率实时监控胞外分泌的神经递质。通过所谓的“净流量”的技术版本，可以在一个给定网站的实际的神经递质浓度计算，这反过来又可以通过质膜转运给神经递质的再摄取率的准确测量。 微透析是在说明在动物之间的不同群体（即不同基因型）和破?...

survivable stereotaxic surgery in rodents 

能够测量大脑清醒动物神经递质外基础水平可确定不同的体制性挑战（药理或生理）中枢神经系统的影响。例如，一个可以直接测量动物的中脑多巴胺预测如何应对像D -苯丙胺或自然的刺激，如食品药物多巴胺的释放。在这段视频中，我们告诉你如何引导套管针对大鼠大脑中的特定的网站植入，如何插入和植入微透析探针，以及如何使用高效液相色谱电化学检测法（HPLC - EC）来衡量外水平可氧化神经递质及代谢产物。通过微透析探针局部药物精确的介绍，让现场特异性的提炼工作，在一个化合物的S作用机制。这种技术具有优良的解剖和化学决议，但只有温和微透析样品的时间分辨率通常处理，每次20-30分钟，以确保检测的神经递质水平。可以帮助补充体内的工具（例如，切片和细胞培养电）监测实时的神经递质。

Watch this Scientific Journal Video about Survivable Stereotaxic Surgery in Rodents at JoVE.com

Survivable Stereotaxic Surgery in Rodents

自由移动的动物不同的大脑区域外的神经递质水平的监测神经递质的释放和行为之间的联系提供了见解。在体内微透析技术结合电化学检测提供了极好的解剖和化学的决议;和基底神经传递是如何由药理或生理操作改变的信息。

在啮齿类动物的存活立体定向手术

The ability to measure extracellular basal levels of neurotransmitters in the brain of awake animals allows for the determination of effects of different ...

Research

JoVE Journal

Biology

Survivable Stereotaxic Surgery in Rodents 

Small Bowel Transplantation In Mice

小鼠小肠移植

Since 1990, the development of tacrolimus-based immunosuppression and improved surgical techniques, the increased array of potent immunosuppressive ...

科研

生物学

A Craniotomy Surgery Procedure for Chronic Brain Imaging

一种慢性脑功能成像开颅手术过程

Imaging techniques are becoming increasingly important in the study brain function. Among them, two-photon laser scanning microscopy has emerged as an ...

Dissecting the Non-human Primate Brain in Stereotaxic Space

剖析在非人类灵长类动物脑立体定向空间

The use of non-human primates provides an excellent translational model for our understanding of developmental and aging processes in humans1-6. In ...

Establishing Primary Adult Fibroblast Cultures From Rodents

建立从小学成人成纤维细胞培养鼠害

The importance of using primary cells, rather than cancer cell lines, for biological studies is becoming widely recognized.  Primary cells are preferred ...

Principles of Rodent Surgery for the New Surgeon

鼠类外科的原则，为新的外科医生

For both scientific and animal welfare reasons, training in basic surgical concepts and techniques should be undertaken before ever seeking to perform ...

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Intracavernosal 压力记录对啮齿动物勃起功能的评价

Erectile dysfunction (ED) is defined as the inability to attain or keep an erection of the penis, and this has become a prevalent male sexual disorder. ...

Inhibition of Wound Epidermis Formation via Full Skin Flap Surgery During Axolotl Limb Regeneration

在蝾螈肢体再生过程中通过全皮瓣手术抑制伤口表皮的形成

Classical experiments in salamander regenerative biology over the last century have long established that the wound epidermis is a crucial signaling ...

Application of Passive Head Motion to Generate Defined Accelerations at the Heads of Rodents

应用被动头部运动在啮齿动物头部产生定义的加速度

Exercise is widely recognized as effective for various diseases and physical disorders, including those related to brain dysfunction. However, molecular ...

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents

在啮齿动物中进行体内和离体电阻抗肌图

Electrical impedance myography (EIM) is a convenient technique that can be used in preclinical and clinical studies to assess muscle tissue health and ...

Anesthesia and Intubation of Preadolescent Mouse Pups for Cardiothoracic Surgery

用于心胸外科的青春期前小鼠幼崽的麻醉和插管

Murine surgical models play an important role in preclinical research. Mechanistic insights into myocardial regeneration after cardiac injury may be ...