Name: protocols for microapplicator-assisted infection of lepidopteran larvae with baculovirus
Uploaded: 2008-08-23T13:32:00
Duration: 10 min 40 s
Description: Watch this Scientific Journal Video about Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus at JoVE.com

S&#39;il vous plaît visitez <a href="http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-59745-457-5_18">Protocoles Springer</a> en apprendre davantage sur le baculovirus d&#39;ingénierie comme un agent insecidical et les techniques de micro-applicateur utilisés dans ce dosage.

The authors have nothing to disclose.

protocols for microapplicator-assisted infection of lepidopteran larvae with baculovirus

Les baculovirus sont largement utilisées comme vecteurs d&#39;expression de protéines et d&#39;insectes agents de lutte antiparasitaire. . Cette vidéo montre comment les larves de lépidoptères peuvent être infectés par des techniques de micro-applicateur dans le tube digestif avec des polyèdres de baculovirus et dans l&#39;hémolymphe par le virus bourgeonne. Ce qui accompagne Springer Protocoles section donne un aperçu du cycle de vie de baculovirus et l&#39;utilisation des baculovirus comme agents insecticides. Formulation et application de baculovirus à des fins de lutte contre les ravageurs sont décrites ailleurs.

Watch this Scientific Journal Video about Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus at JoVE.com

Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

Dans cette vidéo, nous démontrons deux techniques micro-applicateur utilisé pour infecter des larves de lépidoptères avec des baculovirus afin de déterminer l&#39;efficacité insecticide.

Les protocoles de micro-applicateur assistée Infection des larves de lépidoptères avec des baculovirus

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be ...

Research

JoVE Journal

Biology

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Protocoles pour l&#39;infection orale des larves de lépidoptères avec des baculovirus

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. This video shows how lepidopteran larvae can be ...

Recherche

Biologie

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

Méthodes électrophysiologiques pour l&#39;enregistrement du potentiel synaptique de la JNM des larves de drosophile

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. ...

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Protocoles de sélection mutagenèse et fonctionnelles pour l&#39;évolution dirigée de protéines dans les E. coli</em

The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, to create unique molecular ...

Microgavage of Zebrafish Larvae

Microgavage de larves de poisson zèbre

The zebrafish has emerged as a powerful model organism for studying intestinal development1-5, physiology6-11, disease12-16, and host-microbe ...

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Protocoles pour la mise en œuvre d&#39;un<em&gt; Escherichia coli</em&gt; Sur la base du système d&#39;expression cellulaire gratuit TX-TL pour la biologie synthétique

Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform.1 This is useful for ...

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Transfecter des cellules RAW264.7 avec un gène rapporteur de la luciférase

Transfection of desired genetic materials into cells is an inevitable procedure in biomedical research studies. While numerous methods have been ...

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Techniques de micromanipulation permettant l’analyse de la dynamique morphogénétique et chiffre d’affaires des organismes de réglementation du cytosquelette

Examining the spatiotemporal dynamics of proteins can reveal their functional importance in various contexts. In this article, it is discussed how ...

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Protocoles pour tester la toxicité des nouvelles chimies insecticides aux moustiques

New classes of insecticides with novel modes of action are needed to control insecticide resistant populations of mosquitoes that transmit diseases such ...

Forced Salivation As a Method to Analyze Vector Competence of Mosquitoes

Forcé de Salivation comme une méthode pour analyser la compétence vectorielle de moustiques

Vector competence is defined as the potential of a mosquito species to transmit a mosquito-borne virus (mobovirus) to a vertebrate host. Viable virus ...

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Explorer les épitranscriptomes m6A et m5C lors d’une infection virale : un exemple avec le VIH

The role of RNA modifications in biological processes has been the focus of an increasing number of studies in the last few years and is known nowadays as ...