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DOI :
10.3791/899-v
•
11:37 min
September 17th, 2008
Chapters
0:00
Title
1:23
Introduction
1:52
Preparation of Reagents
4:33
Setting Up the Protease Assay and Standard Curves
8:12
Measuring Absorbance and Calculating Enzyme Activity
10:59
Conclusion
プロテアーゼはペプチド結合を切断。ラボでは、それはしばしば測定および/またはプロテアーゼの活性を比較する必要があります。シグマの非特異的プロテアーゼ活性のアッセイは、プロテアーゼの活性を決定するために標準化された手順として使用できます。
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