Name: studying membrane biogenesis with a luciferase-based reporter gene assay
Uploaded: 2008-09-07T14:36:00
Description: Watch this Scientific Journal Video about Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay at JoVE.com

细胞培养<ol><li>人类胚胎肾293（HEK293）细胞联合文化是生长在10 - cm的文化菜“中小型一个”贝科的修改鹰的中等辅以一个抗生素鸡尾酒（100单位每青霉素毫升和100微克（DMEM）组成，每毫升硫酸链霉素）和10％胎牛血清（FBS）。 </li><li>要成立一个实验的细胞，中期是吸，细胞两次洗净，用5毫升磷酸缓冲液（PBS）的解决方案。 </li><li> PBS是然后取出，用0.6毫升含0.25％胰蛋白酶溶液取代。 </li><li>这道...

studying membrane biogenesis with a luciferase-based reporter gene assay

要研究膜生物合成的过程中，协调不同的脂质合成途径，它是有用的工作与实验系统，膜生物合成和诱导的方式迅速发生。我们已经发现，细胞迅速合成膜脂，以补充失去了作为包装材料在颗粒吞噬膜池的乳胶珠的吞噬功能是为这些目的的实际。在这里，我们描述基于荧光素酶报告基因的使用自Promega双GLO的萤光素酶检测系统的方法研究细胞的吞噬功能，诱导基因表达的变化的过程。

Watch this Scientific Journal Video about Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay at JoVE.com

Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

在这里，我们描述基于荧光素酶报告基因的使用自Promega的双GloTM荧光素酶检测系统的方法研究细胞的吞噬功能，诱导基因表达的变化的过程。

研究与基于荧光素酶的记者基因检测膜生源

To study the coordination of different lipid synthesis pathways during membrane biogenesis it is useful to work with an experimental system where membrane ...

Research

JoVE Journal

Biology

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

微流体装置与Groove的模式，为研究细胞行为

We describe a microfluidic device with microgrooved patterns for studying cellular behavior.  This microfluidic platform consists of a top fluidic channel ...

科研

生物学

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

使用基因脉冲MXcell电穿孔系统主细胞的转染效率高

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser ...

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::&beta;-glucuronidase Reporter Gene Expression

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::ÃƒÅ½Ã‚Â²-glucuronidase Reporter Gene Expression

花香浸改造拟南芥检查 pTSO2：β-葡萄糖醛酸酶报告基因的表达

The ability to introduce foreign genes into an organism is the foundation for modern biology and biotechnology. In the model flowering plant Arabidopsis ...

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

农杆菌介导的病毒诱导的基因沉默含量在棉花

Cotton (Gossypium hirsutum) is one of the most important crops worldwide. Considerable efforts have been made on molecular breeding of new varieties.  The ...

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3&#39;UTR记者构造的microRNA识别目标在贴壁细胞

MicroRNAs (miRNAs) are important regulators of gene expression and play a role in many biological processes.  More than 700 human miRNAs have been ...

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统

Glucocorticoid stress hormones and their artificial derivatives are widely used drugs to treat inflammation, but long-term treatment with glucocorticoids ...

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

记者的增长分析的蛋白质降解的系统分析

Protein degradation by the ubiquitin-proteasome system (UPS) is a major regulatory mechanism for protein homeostasis in all eukaryotes. The standard ...

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

转染RAW264.7细胞用荧光素酶报告基因

Transfection of desired genetic materials into cells is an inevitable procedure in biomedical research studies. While numerous methods have been ...

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

在拟南芥原生质体转染昼夜的表型有荧光时钟记者的快速分析

The plant circadian clock allows the anticipation of daily changes to the environment. This anticipation aids the responses to temporally predictable ...

Visualizing Stromule Frequency with Fluorescence Microscopy

可视化Stromule频率与荧光显微镜

Stromules, or "stroma-filled tubules", are narrow, tubular extensions from the surface of the chloroplast that are universally observed in plant cells but ...