Name: making patch-pipettes and sharp electrodes with a programmable puller
Uploaded: 2008-10-08T00:00:00
Description: Watch this Scientific Journal Video about Making Patch-pipettes and Sharp Electrodes with a Programmable Puller at JoVE.com

<p>我们感谢以下资助机构的支持和基础：美国国立卫生研究院，唐纳德B.肌肉萎缩症协会，美国国家科学基金会，美国心脏协会，和迪莉娅E.巴克斯特基金会，Klingenstein基金和神经科学的麦克奈特基金会。 </p>

<p class="jove_step">拉移液器</p><p class="jove_content">电极使用拉马等，作为萨特的P - 97火焰山/布朗，拉约10-20移液器。 </p><ol><li>选择你的毛细血管玻璃。我们使用高硼硅毛细管（萨特BF150 - 110 - 10，1.5毫米外径，内径1.1毫米，长10cm）。商店的玻璃，仔细，所以保持清洁和无尘。 </li><li>设计一个拉动的程序。我们采用5步程序，在每一步，降热和速度和小拉的最后一步。 Sutter的吸管的食谱是一个合适的方案开发很好的参考...

<p>协议说明这里是在电生理实验室的日常使用，也可用于细胞和动物的注射针头。一个可编程的车夫，很容易使多种用途的移液器。重视和关怀，你的车夫长丝将持续一年或一年以上。祝您的实验。 </p>

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Micropipette Puller</td>
<td>Instrument</td>
<td>Sutter Instruments</td>
<td>P-97</td>
<td>Or similar instrument (e.g. Sutter P-87 or P-2000)</td>
</tr>
<tr>
<td>Glass Capillaries</td>
<td>Reagent</td>
<td>Sutter Instruments</td>
<td>BF150-86-10</td>
<td>Or, similar capillary glass.  To make filling the pipette easier, use a capillary with a glass filament.</td>
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making patch-pipettes and sharp electrodes with a programmable puller

<p class="jove_content">玻璃微电极（也称为移液器）已经几十年了电的主力。如今，这样的吸液管是由玻璃毛细管使用一个可编程的车夫。这种仪器的热量使用金属丝或激光，并绘制出重力，马达或两者同时使用玻璃毛细管。膜片钳记录滴管使用唯一的热量和重力形成的，而锋利的电极细胞内记录，用热，重力和电机的组合。用来使细胞内记录移液器的过程是类似的用于各种应用，包括的cRNA注射到非洲爪蟾卵母细胞的注射针头。一般情况下，玻璃毛细管&lt;直径1.2毫米，是用来做膜片钳记录的移液器，而窄的玻璃是用于细胞内记录（外径为1.0毫米）。对于每一个工具，拉马是编程略有不同。该视频演示如何使两种录音移液器使用预先确定的车夫方案。 </p>

Watch this Scientific Journal Video about Making Patch-pipettes and Sharp Electrodes with a Programmable Puller at JoVE.com

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

<p class="jove_content">该视频演示了如何使用一个可编程的车夫电补丁的移液器和锋利的电极。可以使用同样的程序，作出各种玻璃工具，包括注射针头。</p>

膜片吸液管和夏普电极与一个可编程的拖轮

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades.  Today, such pipettes are made from glass capillaries ...

Research

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