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DOI :
10.3791/945-v
November 26th, 2008
Chapters
0:00
Title
0:10
Introduction to Cell Surface Protein Labeling with Ettan DIGE System
1:12
Overview of the Experiment
2:19
Sample Preparation
3:13
Labeling with CyDye DIGE Fluors
4:03
Incubation and Quenching
4:45
Cell Lysis
6:31
First Dimension ISO-Electric Focusing
5:29
Rehydration of IPG Strips
7:27
Gel Casting
9:43
Second Dimension SDS-Page
7:55
Equilibration of IPG Strips
10:29
Gel Scanning
10:52
Results of Cell Surface Protein Labeling
11:21
Comparison with Fractionated Runs
12:30
2D DIGE Analysis
13:27
Conclusions
단순하고 구체적인 방법은 분획화 단계없이 형광 라벨 부착 및 세포 표면 단백질의 향상된 감지를위한 시연했다. 세포 표면 단백질의 차동 풍부한는 (2 - D) 2 차원 전기 영동과 Ettan ™ DIGE 기술을 사용하여 분석했다.
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