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DOI :
10.3791/947-v
September 15th, 2008
Chapters
0:00
Title
0:29
Preparing the lipids
2:13
Determining how much ICAM-1 and MHC are deposited on the planar bilayer
3:22
Cleaning the glass cover slips for forming planar bilayers
4:47
Forming the bilayers
8:27
Charging the bilayers and introducing His-tagged proteins
11:36
Conclusion
10:20
Introducing primary T cells into the flow cell
支持的平面双层功能强大的工具,可以用来模拟在免疫突触的分子间的相互作用。在这里,我们显示锚定细胞粘附蛋白来调节突触的形成脂质bilyer上单张和可视化突触的形成,使用TIRF显微镜的方法。
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