Name: the hypoxic ischemic encephalopathy model of perinatal ischemia
Uploaded: 2008-11-19T00:00:00
Description: Watch this Scientific Journal Video about The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia at JoVE.com

由美国心脏协会和March of Dimes的。

批准该协议是在斯坦福大学和遵守机构的动物护理和使用委员会由国家机构使用实验动物的健康指引。 新生大鼠缺氧缺血性脑病模型<ol><li>产后一天（PND）7只S​​prague - Dawley幼鼠（查尔斯河实验室，公司，威尔明顿，马）完全麻醉与异氟醚（Aerrane，巴克斯特，迪尔菲尔德，IL诱导的3-4％和1-2％维护）。 </li><li>小切口在颈部（超薄虹膜剪刀＃14088-10，精细的科学工具公司，福斯特城，加利?...

<ol>
	<li>Rice, J. E., Vannucci, R. C., Brierley, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+influence+of+immaturity+on+hypoxic-ischemic+brain+damage+in+the+rat.">The influence of immaturity on hypoxic-ischemic brain damage in the rat.</a> Annals of neurology. 9 (2), 131-141 (1981).</li><li>Vannucci, R. C., Vannucci, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perinatal+Hypoxic-Ischemic+Brain+Damage:+Evolution+of+an+Animal+Model.">Perinatal Hypoxic-Ischemic Brain Damage: Evolution of an Animal Model.</a> Dev Neurosci. 27 (2-4), 81-86 (2005).</li><li>Bona, E., Hagberg, H., Loberg, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effects+of+moderate+hypothermia+after+neonatal+hypoxia-ischemia:+short-+and+long-term+outcome.">Protective effects of moderate hypothermia after neonatal hypoxia-ischemia: short- and long-term outcome.</a> Pediatric research. 43 (6), 738-738 (1998).</li><li>Trescher, W. H., Ishiwa, S., Johnston, M. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+hypothermia+and+hyperthermia+on+attentional+and+spatial+learning+deficits+following+neonatal+hypoxia-ischemic+insult+in+rats.">Effects of hypothermia and hyperthermia on attentional and spatial learning deficits following neonatal hypoxia-ischemic insult in rats.</a> Behavioural brain research. 151 (1-2), 209-209 (2004).</li><li>Mishima, K., Ikeda, T., Yoshikawa, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brief+post-hypoxic-ischemic+hypothermia+markedly+delays+neonatal+brain+injury.">Brief post-hypoxic-ischemic hypothermia markedly delays neonatal brain injury.</a> Brain development. 19 (5), 326-326 (1997).</li><li>Young, R. S., Olenginski, T. P., Yagel, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+graded+hypothermia+on+hypoxic-ischemic+brain+damage:+a+neuropathologic+study+in+the+neonatal+rat.">The effect of graded hypothermia on hypoxic-ischemic brain damage: a neuropathologic study in the neonatal rat.</a> Stroke; a journal of cerebral circulation. 14 (6), 929-929 (1983).</li><li>Bederson, J. B., Pitts, L. H., Germano, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+2,3,5-triphenyltetrazolium+chloride+as+a+stain+for+detection+and+quantification+of+experimental+cerebral+infarction+in+rats.">Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats.</a> Stroke; a journal of cerebral circulation. 17 (6), 1304-1304 (1986).</li><li>Cai, J., Kang, Z., Liu, W. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrogen+therapy+reduces+apoptosis+in+neonatal+hypoxia-ischemia+rat+model.">Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model.</a> Neuroscience letters. 441 (2), 167-167 (2008).</li><li>Grow, J., Barks, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogenesis+of+hypoxic-ischemic+cerebral+injury+in+the+term+infant:+current+concepts.">Pathogenesis of hypoxic-ischemic cerebral injury in the term infant: current concepts.</a> Clinics in perinatology. 29 (4), 585-585 (2002).</li><li>Aden, U., Halldner, L., Lagercrantz , H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aggravated+brain+damage+after+hypoxic+ischemia+in+immature+adenosine+A2A+knockout+mice.">Aggravated brain damage after hypoxic ischemia in immature adenosine A2A knockout mice.</a> Stroke; a journal of cerebral circulation. 34 (3), 739-739 (2003).</li></ol>

产后缺氧缺血性脑病的啮齿类动物模型建立的模型，概括脑缺氧，在新生儿围产后期间发生。广泛的组织学和免疫特性的研究表明，7天的老灭鼠有类似的大脑成熟模型孕晚期胎儿7。鼠缺氧缺血性脑病模型已经非常丰富，了解脑损伤的围产后缺氧，低温干预措施，如已证实 3机制。这个模型最初是完善在大鼠和小鼠8最近改编。 值得一提的有具体的技术细节： <ol><li>的CCA结扎之前?...

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<td>Sprague-Dawley Rat Pups</td>
<td>Animal</td>
<td>Charles River Laboratories</td>
<td>&nbsp;</td>
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<td>Isoflurane</td>
<td>Surgery</td>
<td>Aerrane,Baxter</td>
<td>&nbsp;</td>
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<td>8% Oxygen/ 92% Nitrogen Gas</td>
<td>Surgery</td>
<td>Airgas</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>2,3,5-triphenyl tetrazolium chloride </td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>T8877</td>
<td>&nbsp;</td>
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<td>Phosphate buffered saline (PBS) pH 7.4 </td>
<td>Reagent</td>
<td>Gibco/Invitrogen</td>
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<td>Paraformaldehyde</td>
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the hypoxic ischemic encephalopathy model of perinatal ischemia

缺氧缺血性脑病（HIE）是全身出生时发生窒息的后果。二十五％的新生儿缺氧缺血性脑病的发展严重和永久性的神经心理的后遗症，包括精神发育迟滞，脑瘫，癫痫。缺氧缺血性脑病的结果是灾难性的，永久的，使得它的关键识别和制定治疗策略，以减少新生儿缺氧缺血性脑病的脑损伤。为此，新生儿缺氧缺血性脑损伤大鼠模型已开发模型这个人的条件。 Vannucci等1缺氧缺血性脑病模型首次验证，并一直广泛用于识别从围产期缺氧缺血导致的脑损伤的机制，来测试潜在的治疗干预3,4。缺氧缺血性脑病模型是一个两步的过程，涉及暴露在缺氧的环境，左颈总动脉结扎。半球同侧颈动脉结扎脑血流（CBF）的不减少，因为通过Willis环侧支血流;然而，随着氧张力降低，CBF在同侧半球显着降低和单方面缺血性损伤的结果。使用2,3,5 -三苯基氯化四氮唑（TTC）染色，并确定脑组织缺血，最初是成年啮齿动物脑缺血5模型，用于评估在早期的时间点脑infarctin的程度高达72小时后缺血事件6。在这段视频中，我们展示了在产后大鼠脑缺氧缺血损伤模型和使用TTC染色梗死大小的评价。

Watch this Scientific Journal Video about The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia at JoVE.com

The Hypoxic Ischemic Encephalopathy Model of Perinatal Ischemia

产后缺氧缺血性脑损伤大鼠模型，是一个人类新生儿缺氧缺血性脑病（HIE）的行之有效的模式。在这篇文章中，我们描述了在产后幼鼠缺氧缺血性脑病的模型。

围产期缺血缺氧缺血性脑病模型

Hypoxic-Ischemic Encephalopathy (HIE) is the consequence of systemic asphyxia occurring at birth. Twenty five percent of neonates with HIE develop severe ...

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