Name: blood collection from the american horseshoe crab, limulus polyphemus
Uploaded: 2008-10-13T00:00:00
Description: Watch this Scientific Journal Video about Blood Collection from the American Horseshoe Crab, Limulus Polyphemus at JoVE.com

支持原始的上述研究是由美国国家科学基金会资助0344630。

鲎有关的出血的解剖特点（图1） <img src="files/ftp_upload/old/958_2008_10_3_0/958_Figure-01.png" alt="图1" width="389" height="259" /> 图1 <ol><li>人体的三个主要业务部门，由前到后，prosoma（P）opisthosoma（海外），和尾节（T）1。 </li><li> prosoma前和横向的自由空间的法兰。 </li><li> opisthosoma后最压痕，尾节阐述，是终端湾。 </li><li>联合prosoma的opisthosoma口齿...

<ol>
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有4种鲎， 鲎波吕斐摩斯从北美东海岸和三个品种，范围从日本到孟加 ​​拉湾。动物被确定为“活化石”，因为解剖学上的类似形式已为化石发现4.45亿年前27。鲎代表一个长寿命的动物，需要10 - 13岁达到成熟和生活至少20年的 28 。虽然一直受到广泛的收获作为诱饵的鳗鱼，海螺渔业29，美国的鲎仍然相当丰富，并允许50毫升血从一个小的成人非破坏性的收集和，从高达400毫升大?...

blood collection from the american horseshoe crab, limulus polyphemus

鲎的任何最佳的长寿命无脊椎动物的免疫系统。马蹄蟹的免疫研究提供了便利，便于收集大量的血液，并从简单的血液。马蹄蟹只有在一般的流通，颗粒amebocyte一个单一的细胞类型。血浆海水中的盐含量，只有三个丰富的蛋白质，血蓝蛋白，呼吸蛋白，C -反应蛋白，在外来细胞的杀伤破坏的功能，包括细菌细胞和α2-巨球蛋白，能抑制蛋白酶入侵的病原体。血液收集的条件下，心脏穿刺直接脂多糖（又名，内毒素，LPS），革兰氏阴性菌的产品，最大限度地减少污染。大型动物可以产生200 - 400毫升的血液。等离子体的研究，立即清除血细胞从血浆离心的血浆，然后可以分割成它的组成蛋白质。方便的血细胞是研究微观LPS无等渗盐水（0.5 M氯化钠）的条件下，允许直接镜检培养皿的表面上放置一个多脂多糖免费coverglasses通过收集小体积血，然后安装这些coverglasses在简单的观察室，下面的细胞附着。一个直接观察的第二准备，以收集3 - 5毫升血液，在LPS无胚盘，然后explanting片段的聚合amebocytes一腔三明治组织之间的幻灯片和一个玻璃罩。在这个准备，运动型amebocytes迁移到盖玻片表面，在那里他们可以很容易地观察到。凝血系统涉及amebocytes聚集和形成血块，这是由血细胞分泌颗粒释放的一种蛋白质，coagulin外。洗血细胞的生化分析的需要，聚集和脱颗粒不会发生，它可以通过收集到0.1 2％的Tween - 20，0.5 M的LPS无盐，其次是细胞离心和洗涤用0.5的中号的卷的血液完成氯化钠。

Watch this Scientific Journal Video about Blood Collection from the American Horseshoe Crab, Limulus Polyphemus at JoVE.com

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

美国鲎，鲎波吕斐摩斯，无疑是最方便的任何无脊椎动物的血液大量源。血液成分简单，只有一个循环，颗粒amebocyte，只有三个蛋白质丰富的血浆，血蓝蛋白，C -反应蛋白和α2-巨球蛋白的细胞类型。发自内心的血液收集和离心分离血细胞和血浆。

从美国的马蹄蟹，鲎波吕斐摩斯采血车

The horseshoe crab has the best-characterized immune system of any long-lived invertebrate.  The study of immunity in horseshoe crabs has been facilitated ...

Research

JoVE Journal

Biology

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

NK细胞与干细胞技术的RosetteSep套件的人体血液的富集

Natural killer (NK) cells are large granular cytotoxic lymphocytes that belong to the innate immune system and play major roles in fighting against cancer ...

科研

生物学

A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse 

一个串行收集脑脊液从池鼠标麦格纳技术

Alzheimer's disease (AD) is a progressive neurodegenerative disease that is pathologically characterized by extracellular deposition of &#946;-amyloid ...

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

马蹄蟹，鲎波吕斐摩斯，在视觉研究

The American horseshoe crab, Limulus Polyphemus is one of the oldest creatures on earth, and the animal continues to play an indispensable role in ...

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

从新生小鼠的肺血管内皮细胞的分离和培养

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

Blood Collection for Biochemical Analysis in Adult Zebrafish

在成年斑马鱼的生化分析采血车

The zebrafish has been used as an animal model for studies of several human diseases. It can serve as a powerful preclinical platform for studies of ...

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

一种实用的新的方法来提取基因组DNA保存血浆蛋白从血液采集套件

Laboratory tests can be done on the cellular or  fluid portions of the blood. The use of different blood collection tubes  determines the portion of the ...

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

从环境中自然产生的趋磁细菌的采集，分离和富集

Magnetotactic bacteria (MTB) are aquatic microorganisms that were first notably described in 19751 from sediment samples collected in salt marshes of ...

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

从血液高效的iPS细胞生成使用附加体和HDAC抑制剂

This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using ...

Sampling Blood from the Lateral Tail Vein of the Rat

从大鼠的侧尾静脉血液取样

Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

Repeated Blood Collection for Blood Tests in Adult Zebrafish

重复采血车在成年斑马鱼验血

Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for blood collection from ...