Name: blood collection from the american horseshoe crab, limulus polyphemus
Uploaded: 2008-10-13T00:00:00
Description: Watch this Scientific Journal Video about Blood Collection from the American Horseshoe Crab, Limulus Polyphemus at JoVE.com

위에서 설명한 기존 연구는 NSF 교부금 0,344,630에 의해 지원되었다.

출혈과 관련된 말굽 게의 해부 학적 특징 (그림 1) <img src="files/ftp_upload/old/958_2008_10_3_0/958_Figure-01.png" alt="그림 1" width="389" height="259" /> 그림 1 <ol><li> 신체의 세 가지 주요 부문은 사후에 앞쪽에에서 prosoma (P), opisthosoma (O), 그리고 텔슨 (T) 1입니다. </li><li> prosoma의 앞쪽에과 측면 무료 마진은 플랜지입니다. </li><li> 텔슨은 articulates opisthoso...

<ol>
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H., Levin, J., Holme, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+studies+of+the+granules+of+the+amebocytes+of+Limulus+polyphemus,+the+horseshoe+crab.">Isolation and studies of the granules of the amebocytes of Limulus polyphemus, the horseshoe crab.</a> J. Cell Physiol. 86, 533-542 (1975).</li><li>Decker, H., Ryan, M., Jaenicke, E., Terwilliger, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SDS+induced+phenoloxidase+activity+of+hemocyanins+from+Limulus+polyphemus,+Eurypelma+californicum+and+cancer.">SDS induced phenoloxidase activity of hemocyanins from Limulus polyphemus, Eurypelma californicum and cancer.</a> , 276-17796 (2001).</li><li>Armstrong, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+cytolytic+function+for+a+sialic+acid-binding+lectin+that+is+a+member+of+the+pentraxin+family+of+proteins.">A cytolytic function for a sialic acid-binding lectin that is a member of the pentraxin family of proteins.</a> J. Biol. Chem. 271, 14717-14721 (1996).</li><li>Armstrong, P. B., Melchior, R., Quigley, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Humoral+immunity+in+long-lived+arthropods.">Humoral immunity in long-lived arthropods.</a> J. Insect Physiol. 42, 53-64 (1996).</li><li>Armstrong, P. B., Armstrong, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+decorated+clot:+Binding+of+agents+of+the+innate+immune+system+to+the+fibrils+of+the+limulus+blood+clot.">The decorated clot: Binding of agents of the innate immune system to the fibrils of the limulus blood clot.</a> Biol. Bull. 205, 201-203 (2003).</li><li>Armstrong, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interaction+of+the+motile+blood+cells+of+the+horseshoe+crab,+Limulus.+Studies+on+contact+paralysis+of+pseudopodial+activity+and+cellular+overlapping+in+vitro.">Interaction of the motile blood cells of the horseshoe crab, Limulus. Studies on contact paralysis of pseudopodial activity and cellular overlapping in vitro.</a> Exp. Cell Res. 107, 127-138 (1977).</li><li>Armstrong, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Motility+of+the+Limulus+blood+cell.">Motility of the Limulus blood cell.</a> J. Cell Sci. 37, 169-180 (1979).</li><li>Armstrong, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adhesion+and+spreading+of+Limulus+blood+cells+on+artificial+surfaces.">Adhesion and spreading of Limulus blood cells on artificial surfaces.</a> J. Cell Sci. 44, 243-262 (1980).</li><li>Armstrong, P. B., Cohen, W. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+Cells+of+Marine+Invertebrates.">Blood Cells of Marine Invertebrates.</a> Lab. Animal. , 253-258 (1985).</li><li>Smith, S. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+oldest+horseshoe+crab:+a+new+xiphosurid+from+Late+Ordovician+Konservat-Lagerstatten+deposits.">The oldest horseshoe crab: a new xiphosurid from Late Ordovician Konservat-Lagerstatten deposits.</a> Palaeontology. 51, 1-9 (2008).</li><li>Shuster, C. N., Sekiguchi, K., Shuster, C. N., Barlow, R. B., Brockmann, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The American Horseshoe Crab. , 103-132 (2003).</li><li>Walls, E. A., Berkam, J., Smith, S. 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말굽 게 4 종의, 동쪽의 북아메리카 해안 세 종 일본에서 벵골 만 해당 범위에서 Limulus polyphemus의가 있습니다. 해부학적인 몸의 구조와 유사한 형태가 27 4억4천5백만년 전에서 화석으로 발견 되었기 때문에 동물은 &quot;살아있는 화석&quot;으로 식별됩니다. 성숙에 도달 13 년 최소한 20 년은 28 생활 - 말굽 게 10을 필요로하는, 긴 동물을 나타냅니다. 그것은 장어와 콘치 어업 29 미끼로 ?...

blood collection from the american horseshoe crab, limulus polyphemus

말굽 게 어떤 긴 무척추 동물의 가장 특징 면역 체계가 있습니다. 말굽 게는에서 면역의 연구는 혈액의 대량 수집 용이성으로하고 혈액의 단순에서 촉진되었다. 말굽 게는 일반적인 순환, 세분화 amebocyte에서만 하나의 셀 유형을 보여줍니다. 플라즈마는 바닷물의 염분 성분을 가지고 있으며, 단지 세 풍부한 단백질, hemocyanin, 호흡기 단백질, C - 반응 박테리아 세포를 포함한 외부 세포의 cytolytic 파괴 기능 단백질, 및 α2 - macroglobulin은 프로 테아제를 억제하는 병원균을 침해니다. 혈액은 lipopolysaccharide (일명, 내독소, LPS), 그람 음성 세균의 제품으로 오염을 최소화 조건 하에서 직접 심장 주사에 의해 수집됩니다. 혈액 400 ML - 대형 동물은 200를 얻을 수 있습니다. 플라즈마의 연구, 혈액 세포는 즉시 원심 분리에 의해 혈장에서 제거되며 플라즈마 다음의 구성 단백질로 fractionated 수 있습니다. 혈액 세포가 편리하게 다음 그 coverglasses를 장착, 문화 접시 표면에 대한 자세한 중 하나 LPS 무료 coverglasses를 삽입하여 직접 현미경 검사를 허용 조건 LPS 무료 isotonic 호수 (0.5 M NaCl)에 혈액 작은 볼륨을 수집하여 미세 공부 아르 세포 부착 다음과 같은 간단한 관측 실 인치 직접 관찰을위한 두 번째 준비 3를 수집하는 것입니다 - LPS없는 배아 요리의 혈액 5 ML하고 다음 실로 총체적 amebocytes의 조각을 explanting하는 슬라이드와 coverglass 사이에 샌드위치가 조직. 이 준비에서 운동성이있는 amebocytes 그들이 쉽게 볼 수 있습니다 coverglass 표면에,에 마이 그 레이션. 혈액 응고 시스템 amebocytes의 집합과 혈액 세포의 분비 과립의에서 발표되는 단백질, coagulin의 세포외 응고의 형성을 포함한다. 세탁 혈액 세포의 생화 학적 분석 2퍼센트 트윈 - 20, 0.5 M LPS 무료 NaCl, 세포의 원심 분리에 의해 다음과 0.5로 세척 M의 0.1 볼륨으로 혈액을 수집하여 수행할 수 수 집합과 degranulation가 발생하지 않습니다을 필요로 NaCl.

Watch this Scientific Journal Video about Blood Collection from the American Horseshoe Crab, Limulus Polyphemus at JoVE.com

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

미국 말굽 게, Limulus polyphemus은 틀림없이 모든 무척추 동물의 혈액의 다량을 위해 가장 편리한 소스입니다. 혈액의 일반적인 순환, 세분화 amebocyte, 그리고 플라즈마, hemocyanin, C - 반응 단백질 및 α2 - macroglobulin에서 3 풍부한 단백질에 하나의 셀 유형, 구성의 간단하다. 혈액은 심장에서 수집되고 혈액 세포 및 플라즈마 원심 분리로 구분됩니다.

미국 호르세쇼 게, Limulus Polyphemus의 혈액 수집

The horseshoe crab has the best-characterized immune system of any long-lived invertebrate.  The study of immunity in horseshoe crabs has been facilitated ...

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Biology

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

StemCell 기술에서 RosetteSep 키트와 함께 인간의 피를에서 NK 세포의 농축

Natural killer (NK) cells are large granular cytotoxic lymphocytes that belong to the innate immune system and play major roles in fighting against cancer ...

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A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse 

Cisterna 마그나 마우스에서 뇌척수의 직렬 그룹에 대한 기술

Alzheimer's disease (AD) is a progressive neurodegenerative disease that is pathologically characterized by extracellular deposition of &#946;-amyloid ...

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

호르세쇼 크랩을 사용하여, Limulus Polyphemus 비전 연구

The American horseshoe crab, Limulus Polyphemus is one of the oldest creatures on earth, and the animal continues to play an indispensable role in ...

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

신생아 마우스의 폐 내피 세포의 분리 및 문화

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

Blood Collection for Biochemical Analysis in Adult Zebrafish

성인 Zebrafish의 생물 화학 분석을위한 혈액 수집

The zebrafish has been used as an animal model for studies of several human diseases. It can serve as a powerful preclinical platform for studies of ...

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

플라즈마 단백질 보존을위한 혈액 수집 장비로부터 게놈 DNA를 추출하는 실제적이고 새로운 방법

Laboratory tests can be done on the cellular or  fluid portions of the blood. The use of different blood collection tubes  determines the portion of the ...

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

당연히 환경에서 Magnetotactic 박테리아를 발생의 수집, 절연 및 강화

Magnetotactic bacteria (MTB) are aquatic microorganisms that were first notably described in 19751 from sediment samples collected in salt marshes of ...

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

에피 솜과 HDAC 억제제를 사용하여 혈액에서 효율적인 유도 만능 줄기 세포 생성

This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using ...

Sampling Blood from the Lateral Tail Vein of the Rat

쥐의 측면 꼬리 정맥에서 혈액을 샘플링

Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

Repeated Blood Collection for Blood Tests in Adult Zebrafish

성인 Zebrafish의에서 혈액 검사에 대한 반복 채혈

Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for blood collection from ...