Name: blood collection from the american horseshoe crab, limulus polyphemus
Uploaded: 2008-10-13T00:00:00
Description: Watch this Scientific Journal Video about Blood Collection from the American Horseshoe Crab, Limulus Polyphemus at JoVE.com

Yukarıda anlatılan özgün araştırma, NSF hibe 0344630 tarafından desteklenen oldu.

Kanama ile ilgili at nalı yengeci anatomik özellikleri (Şekil 1) <img src="files/ftp_upload/old/958_2008_10_3_0/958_Figure-01.png" alt="Şekil 1" width="389" height="259" /> Şekil 1 <ol><li> Vücudun üç ana bölümden posterior anterior (P), zehir opisthosoma (O), ve telzon (T) 1. </li><li> Zehir anterior ve lateral kenar boşlukları flanş. </li><li> Telzon dile opisthosoma en arka girinti, terminal koy. </li><li> Zehir ve opis...

<ol>
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At nalı yengeci, Kuzey Amerika&#39;nın doğu kıyıları ve Bengal Körfezi, Japonya bu aralıkta üç türün Limulus Polyphemus&#39;un dört tür vardır . Anatomik benzeri şekillerde 27 445 milyon yıl önce fosil olarak tespit edilmiştir, çünkü hayvan bir &quot;yaşayan fosil&quot; olarak tanımlanır. Olgunluğa ulaşması 13 yıl ve en az 20 yıl 28 yaşayan at nalı yengeci, 10 gerektiren, uzun ömürlü bir hayvan temsil eder. Amerikan at nalı yengeci, yılan balığı ve kabuklu balıkç?...

blood collection from the american horseshoe crab, limulus polyphemus

At nalı yengeci, herhangi bir uzun ömürlü omurgasız en iyi karakterize bağışıklık sistemine sahiptir. At nalı yengeçleri bağışıklık çalışma, büyük miktarda kan toplama kolaylığı ve kan basitlik kolaylaştırılmıştır. At nalı yengeçleri, genel dolaşımı, granül amebocyte sadece tek bir hücre tipi göstermektedir. Plazma, deniz suyunun tuz içeriğine sahiptir ve sadece üç bol miktarda protein, hemosiyanin, solunum protein, C-reaktif bakteri hücreleri de dahil olmak üzere, yabancı hücrelerin sitolitik imha işlevini proteinleri, ve α2-makroglobulin, proteazlar inhibe patojenlerin işgalci. Kan lipopolisakkarid (aka, endotoksin, LPS), Gram-negatif bakteriler bir ürün kontaminasyonu en aza indirecek koşullar altında doğrudan kalp ponksiyon tarafından toplanır. 400 mL kan büyük bir hayvan 200 verim alınabilir. Plazma, kan hücrelerinin çalışma için santrifüj ile plazmadan hemen kaldırılır ve plazma sonra kendisini oluşturan proteinleri içine fraksiyone olabilir. Rahatlıkla kan hücrelerinin LPS-izotonik tuzlu su (0,5 M NaCl) içine küçük miktarda kan kültürü çanak yüzeyinde daha fazla bir LPS-ücretsiz coverglasses koyarak direkt mikroskobik muayene izin koşullar altında toplayarak, daha sonra bu coverglasses montaj mikroskobik olarak incelenmiştir hücre eklenti aşağıdaki basit gözlem odalarında. Doğrudan gözlem için ikinci bir hazırlık toplamak için 3 - 5 ml kan LPS-embriyo çanak ve sonra bir odaya toplanmış amebocytes parçaları explanting bir slayt ve coverglass arasında sandviç doku. Bu hazırlık, hareketli amebocytes kolayca görülebilir coverglass yüzey üzerine göç ederler. Kanın pıhtılaşma sisteminde amebocytes agregasyonu ve kan hücrelerinin salgı granülleri salınan bir protein, coagulin ekstrasellüler bir pıhtı oluşumunu içerir. % 2 Tween-20, 0.5 M LPS-free NaCl, hücreler santrifüj tarafından takip ve 0.5 ile yıkanarak M 0.1 birimlere kan toplama gerçekleştirilebileceğini agregasyonu ve degranülasyonu oluşmaz, yıkanmış kan hücrelerinin biyokimyasal analiz gerektirir NaCl.

Watch this Scientific Journal Video about Blood Collection from the American Horseshoe Crab, Limulus Polyphemus at JoVE.com

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

Amerikan at nalı yengeci, Limulus Polyphemus&#39;un, tartışmalı, büyük miktarda herhangi bir omurgasız kan için en uygun bir kaynağıdır. Kan genel dolaşımı, granül amebocyte ve plazma, hemosiyanin, C-reaktif protein ve α2-makroglobulin sadece üç bol protein sadece tek bir hücre tipi, kompozisyon basit. Kan kalpten toplanır ve kan hücreleri ve plazma santrifüj ile ayrılır.

Amerikan Horseshoe Yengeç, Limulus Polyphemus&#39;un Kan Toplama

The horseshoe crab has the best-characterized immune system of any long-lived invertebrate.  The study of immunity in horseshoe crabs has been facilitated ...

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Biology

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Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

Horseshoe Yengeç kullanarak, Limulus Polyphemus&#39;un Vizyon Araştırma

The American horseshoe crab, Limulus Polyphemus is one of the oldest creatures on earth, and the animal continues to play an indispensable role in ...

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

Yenidoğan Fareler Pulmoner Endotel Hücreleri İzolasyon ve Kültür

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

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Yetişkin Zebra balığı Biyokimyasal Analiz için Kan Alma

The zebrafish has been used as an animal model for studies of several human diseases. It can serve as a powerful preclinical platform for studies of ...

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Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

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Magnetotactic bacteria (MTB) are aquatic microorganisms that were first notably described in 19751 from sediment samples collected in salt marshes of ...

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Epizomlar ve HDAC inhibitörleri kullanarak Blood Verimli iPS Hücre Üretimi

This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using ...

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Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for blood collection from ...