Name: collecting and measuring nociceptive and inflammatory mediators in surgical wounds
Uploaded: 2008-10-29T00:00:00
Description: Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

ويدعم عمل الدكتور كارفالو في وظائف عن طريق البناء المتعدد التخصصات في صحة المرأة منحة بحثية من مكتب بحوث صحة المرأة والمعهد الوطني لصحة الطفل والتنمية البشرية التابع لمعاهد الصحة القومية (5K12 HD043452). استقبل الدكتور أنغست اللوازم (وعلى سؤال PainBuster ® ® تخفيف الآلام بعد العملية النظام) والتمويل لإجراء فحوصات البيوكيميائية من تدفق - I (بحيرة الغابات ، كاليفورنيا).

Nociceptive والتهابات جمع الوسيط البيوكيميائية <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> يتم إدراج على سؤال الألم ® ® PainBuster نظام الإغاثة في طبقة تحت الجلد من قبل الفريق الجراحي فقط قبل اغلاق الجرح. نظام يسلم باستمرار المالحة العادي (أو مخدر موضعي) تحت الجلد في الجر?...

<ol>
	<li>Elshal, M. F., McCoy, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assays:+performance+evaluation+and+comparison+of+sensitivity+to+ELISA.">Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA.</a> Methods. 38, 317-323 (2006).</li><li>Heijmans-Antonissen, C., Wesseldijk, F., Munnikes, R. J., Huygen, F. J., van der Meijden, P., Hop, W. C., Hooijkaas, H., Zijlstra, F. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assay+for+detection+of+25+soluble+cytokines+in+blister+fluid+of+patients+with+complex+regional+pain+syndrome+type+1.">Multiplex bead array assay for detection of 25 soluble cytokines in blister fluid of patients with complex regional pain syndrome type 1.</a> Mediators Inflamm.. 28398, 1-8 (2006).</li><li>Buvanendran, A., Kroin, J. S., Berger, R. A., Hallab, N. J., Saha, C., Negrescu, C., Moric, M., Caicedo, M. S., Tuman, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Upregulation+of+prostaglandin+E2+and+interleukins+in+the+central+nervous+system+and+peripheral+tissue+during+and+after+surgery+in+humans.+.">Upregulation of prostaglandin E2 and interleukins in the central nervous system and peripheral tissue during and after surgery in humans. .</a> Anesthesiology. 104, 403-410 (2000).</li><li>Holzheimer, R. G., Steinmetz, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+and+systemic+concentrations+of+pro-+and+anti-inflammatory+cytokines+in+human+wounds.">Local and systemic concentrations of pro- and anti-inflammatory cytokines in human wounds.</a> Eur J Med Res. 5, 347-355 (2000).</li></ol>

وينبغي أن تدرج على سؤال الألم ® ® PainBuster نظام الإغاثة عبر شق في طبقة تحت الجلد بأكمله فقط قبل اغلاق الجرح. يسهل هذا الطموح من خلال محبس ثلاثي في ​​فترات زمنية محددة. وعلى سؤال ® نظام يسلم باستمرار المالحة تحت الجلد الطبيعي في الجرح بمعدل 2 مل / ساعة. هذا يمنع تخثر القسطرة ويحسن من ا?...

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded.
A fifth reference:
 5. Carvalho, B., Clark, D. J. &amp; Angst, M. S. Local and Systemic Release of Cytokines, Nerve Growth Factor, Prostaglandin E2, and Substance P in Incisional Wounds and Serum Following Cesarean Delivery. The Journal of Pain : official journal of the American Pain Society 9 (7), 650-657 (2008).
 was added. The abstract was updated to :
We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. Collecting site-specific biochemical markers allows us to evaluate the relationship between surgical wound and serum levels; determine any associations between mediator release, pain and analgesic consumption; and evaluate the effect of systemic and peripheral drug administration on surgical wound biochemistry.
This methodology has been applied to healthy women undergoing elective cesarean delivery with spinal anesthesia. Wound exudate and serum mediators, in conjunction with pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean delivery. Biochemical mediators that were detected included IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. We found no correlations between wound and serum cytokines concentrations or time-release profiles (J Pain. 2008 Jul 9(7):650-7). This article describes and demonstrates the feasibility of collecting and assaying nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between serum and wound levels shows the importance of determining site-specific release if surgical wounds and localized pathologies are to be studied.
from
The objectives of this study were to test the feasibility of collecting and measuring inflammatory and nociceptive biochemical mediators at the surgical site; to evaluate the relationship between wound and serum levels; and to determine any associations between mediator release, pain and analgesic consumption post-cesarean delivery. Twenty healthy women undergoing elective cesarean delivery with spinal anesthesia were enrolled. Wound exudate and serum mediators, pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean. In wound exudate, 19 out of 20 mediators were reliably detected including IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. Wound PG-E2 and various cytokines peaked early, whereas NGF showed a more delayed release. There were no correlations between the concentration versus time profile of wound and serum cytokines. This study demonstrates the feasibility of collecting and measuring nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between wound and serum levels emphasizes the importance of determining site-specific release if localized pathologies are to be studied.

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded. A revised abstract and the inclusion of a reference for figure 1 is republished here.

Erratum: Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>On-Q&reg; PainBuster&reg; Post-Op Pain Relief System </td>
<td>&nbsp;</td>
<td>I-Flow, Lake Forest, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Complete proteinase inhibitor </td>
<td>&nbsp;</td>
<td>Roche Bioscience, Palo Alto, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>17-multiplex bead immunoassay Bio-PlexTM plate</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Bio-Plex amine coupling kit</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>The NGF antibody DY256</td>
<td>&nbsp;</td>
<td>R&D Systems, Minneapolis, MN</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Prostaglandin E2 and substance P ELISA Kits </td>
<td>&nbsp;</td>
<td>Assay Designs, Inc., Ann Arbor, Michigan</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

collecting and measuring nociceptive and inflammatory mediators in surgical wounds 

وكانت أهداف هذه الدراسة لاختبار جدوى جمع وقياس وسطاء التهابات والبيوكيميائية nociceptive في موقع الجراحة ، وتقييم العلاقة بين الجرح ومستويات المصل ، وتحديد أي علاقة بين الافراج عن الوسيط والألم واستهلاك المسكنات بعد القيصرية التسليم. تم تسجيل عشرين امرأة سليمة تمر الولادة القيصرية الاختيارية مع التخدير الشوكي. تم قياس الافرازات الجرح وسطاء المصل ، درجات الألم والمسكنات الاستهلاك بنسبة 6 ، 1 ، 24 ، و 48 ساعة بعد القيصرية. في الافرازات الجرح ، تم الكشف عن 19 من أصل موثوق بها وسطاء بينهم 20 IL - 1β ، IL - 2 ، IL - 4 ، IL - 6 ، IL - 7 ، IL - 8 ، IL - 10 ، IL - 12 ، IL - 13 ، IL -17 ، TNFα ، INFγ ، G - CSF ، جنرال موتورز CSF ، 1 - MCP وMIP 1β ، عامل نمو الاعصاب (NGF) ، E2 البروستاجلاندين (PG - E2) ، وبلغت ذروتها مادة P. بالجروح PG - E2 والسيتوكينات المختلفة في وقت مبكر ، في حين أظهرت NGF بيان تأخر أكثر. لم تكن هناك علاقة بين تركيز مقابل التعريف وقت الجرح والسيتوكينات المصل. هذه الدراسة تدل على جدوى جمع وقياس وسطاء nociceptive والتهابات في الجروح الجراحية في نقطة زمنية محددة. عدم وجود ارتباط كبير بين مستويات المصل الجرح ويؤكد على أهمية تحديد مواقع محددة بالإفراج عنه إذا المترجمة هي الأمراض التي يتعين دراستها.

Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

وهناك تقنية لجمع وقياس وسطاء الجروح الجراحية البيوكيميائية في نقطة زمنية محددة.

جمع وقياس وسطاء Nociceptive والتهاب في الجروح الجراحية

We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. ...

Research

JoVE Journal

Biology

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds 

Measuring Exocytosis in Neurons Using FM Labeling

قياس إيماس في الخلايا العصبية عن طريق وصفها وزير الخارجية

The ability to measure the kinetics of vesicle release can help provide insight into some of the basics of neurotransmission.  Here we used real-time ...

Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators

وفي الإنسان ، فيفو الأحيائي لقياس محددة من الأنسجة وسطاء Nociceptive التهابية

This in-vivo human bioassay can be used to study human volunteers and patients. Samples are collected from pertinent tissue sites such as the skin via ...

Measuring Fast Calcium Fluxes in Cardiomyocytes

قياس الكالسيوم السريع في تدفقاتها العضلية

Cardiomyocytes have multiple Ca2+ fluxes of varying duration that work together to optimize function 1,2. Changes in Ca2+ activity in response to ...

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

قياس المكانية والزمانية كاليفورنيا<sup&gt; 2+</sup&gt; إشارات في النباتات نبات الأرابيدوبسيس

Developmental and environmental cues induce Ca2+ fluctuations in plant cells. Stimulus-specific spatial-temporal Ca2+ patterns are sensed by cellular Ca2+ ...

Measuring Glutathione-induced Feeding Response in Hydra

قياس الاستجابة التغذية الناجم عن الجلوتاثيون في حيدرة

Hydra is among the most primitive organisms possessing a nervous system and chemosensation for detecting reduced glutathione (GSH) for capturing the prey. ...

Measuring Pressure Volume Loops in the Mouse

قياس حجم حلقات الضغط في الماوس

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the ...

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

قياس صلابة من<em&gt; فيفو السابقين</em&gt; ماوس Aortas باستخدام مجهر القوة الذرية

Arterial stiffening is a significant risk factor and biomarker for cardiovascular disease and a hallmark of aging. Atomic force microscopy (AFM) is a ...

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

الفئران الأرواح سحق إصابة: كفاءة<em&gt; في فيفو</em&gt; نموذج من انتشار الخلايا العضلية السلس والوظيفة البطانية

Arterial reconstruction, whether angioplasty or bypass surgery, involves iatrogenic trauma causing endothelial disruption and vascular smooth muscle cell ...

Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux

قياس الإيقاعات اليومية في التدفق التلقائي والبروتياسومال

Cells employ several methods for recycling unwanted proteins and other material, including lysosomal and non-lysosomal pathways. The main ...

Measuring RAN Peptide Toxicity in C. elegans

Measuring RAN Peptide Toxicity in C. elegans

قياس سمية الببتيد RAN في C. elegans

C. elegans is commonly used to model age-related neurodegenerative diseases caused by repeat expansion mutations, such as Amyotrophic Lateral Sclerosis ...