Name: collecting and measuring nociceptive and inflammatory mediators in surgical wounds
Uploaded: 2008-10-29T00:00:00
Description: Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

卡瓦略博士的工作是支持由妇女健康研究资助妇女的健康和国家的儿童健康和人类发展的美国国立卫生研究院（5K12 HD043452）研究所的研究办公室的跨学科人才招聘大厦。焦虑医生收到用品（ON - Q ® PainBuster ®运算后疼痛缓解系统）和资金进行从我流（CA）的森林湖，生化分析。

伤害性和炎症的生化调解员收集<ol><li> ON - Q ® PainBuster ®止痛系统插入皮下层由手术队前伤口缝合。该系统持续皮下伤口在2毫升/小时的速度提供生理盐水（或局部麻醉） </li><li>一个三路阀被纳入这个系统允许在指定的时间点伤口渗出的愿望。 </li><li>在指定的时间点协议（例如，1，6，24，和剖宫产分娩后48小时），伤口渗出液1毫升撤回到聚乙烯杯含30μL蛋白酶抑制剂。 </li><li>在相同的时间间隔?...

<ol>
	<li>Elshal, M. F., McCoy, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assays:+performance+evaluation+and+comparison+of+sensitivity+to+ELISA.">Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA.</a> Methods. 38, 317-323 (2006).</li><li>Heijmans-Antonissen, C., Wesseldijk, F., Munnikes, R. J., Huygen, F. J., van der Meijden, P., Hop, W. C., Hooijkaas, H., Zijlstra, F. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assay+for+detection+of+25+soluble+cytokines+in+blister+fluid+of+patients+with+complex+regional+pain+syndrome+type+1.">Multiplex bead array assay for detection of 25 soluble cytokines in blister fluid of patients with complex regional pain syndrome type 1.</a> Mediators Inflamm.. 28398, 1-8 (2006).</li><li>Buvanendran, A., Kroin, J. S., Berger, R. A., Hallab, N. J., Saha, C., Negrescu, C., Moric, M., Caicedo, M. S., Tuman, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Upregulation+of+prostaglandin+E2+and+interleukins+in+the+central+nervous+system+and+peripheral+tissue+during+and+after+surgery+in+humans.+.">Upregulation of prostaglandin E2 and interleukins in the central nervous system and peripheral tissue during and after surgery in humans. .</a> Anesthesiology. 104, 403-410 (2000).</li><li>Holzheimer, R. G., Steinmetz, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+and+systemic+concentrations+of+pro-+and+anti-inflammatory+cytokines+in+human+wounds.">Local and systemic concentrations of pro- and anti-inflammatory cytokines in human wounds.</a> Eur J Med Res. 5, 347-355 (2000).</li></ol>

ON - Q ® PainBuster ®止痛系统应横跨整个皮下层切口插入前伤口缝合。这有利于通过在指定的时间间隔三路阀的愿望。 ON - Q ®系统不断传递到伤口的生理盐水皮下注射2毫升/小时的速度这可以防止导管凝血，提高了系统的可靠性产生渗出液样本。抽吸渗液如果困难（约5％的情况下），考虑改变主体的位置（例如，坐在病人最多，或让它们平铺），推伤口上方轻轻，使用一个0.5 -1毫升正常生理盐水冲洗或撤回导管1-2...

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded.
A fifth reference:
 5. Carvalho, B., Clark, D. J. &amp; Angst, M. S. Local and Systemic Release of Cytokines, Nerve Growth Factor, Prostaglandin E2, and Substance P in Incisional Wounds and Serum Following Cesarean Delivery. The Journal of Pain : official journal of the American Pain Society 9 (7), 650-657 (2008).
 was added. The abstract was updated to :
We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. Collecting site-specific biochemical markers allows us to evaluate the relationship between surgical wound and serum levels; determine any associations between mediator release, pain and analgesic consumption; and evaluate the effect of systemic and peripheral drug administration on surgical wound biochemistry.
This methodology has been applied to healthy women undergoing elective cesarean delivery with spinal anesthesia. Wound exudate and serum mediators, in conjunction with pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean delivery. Biochemical mediators that were detected included IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. We found no correlations between wound and serum cytokines concentrations or time-release profiles (J Pain. 2008 Jul 9(7):650-7). This article describes and demonstrates the feasibility of collecting and assaying nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between serum and wound levels shows the importance of determining site-specific release if surgical wounds and localized pathologies are to be studied.
from
The objectives of this study were to test the feasibility of collecting and measuring inflammatory and nociceptive biochemical mediators at the surgical site; to evaluate the relationship between wound and serum levels; and to determine any associations between mediator release, pain and analgesic consumption post-cesarean delivery. Twenty healthy women undergoing elective cesarean delivery with spinal anesthesia were enrolled. Wound exudate and serum mediators, pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean. In wound exudate, 19 out of 20 mediators were reliably detected including IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. Wound PG-E2 and various cytokines peaked early, whereas NGF showed a more delayed release. There were no correlations between the concentration versus time profile of wound and serum cytokines. This study demonstrates the feasibility of collecting and measuring nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between wound and serum levels emphasizes the importance of determining site-specific release if localized pathologies are to be studied.

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded. A revised abstract and the inclusion of a reference for figure 1 is republished here.

Erratum: Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

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<td>On-Q&reg; PainBuster&reg; Post-Op Pain Relief System </td>
<td>&nbsp;</td>
<td>I-Flow, Lake Forest, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Complete proteinase inhibitor </td>
<td>&nbsp;</td>
<td>Roche Bioscience, Palo Alto, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>17-multiplex bead immunoassay Bio-PlexTM plate</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Bio-Plex amine coupling kit</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>The NGF antibody DY256</td>
<td>&nbsp;</td>
<td>R&D Systems, Minneapolis, MN</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Prostaglandin E2 and substance P ELISA Kits </td>
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collecting and measuring nociceptive and inflammatory mediators in surgical wounds 

这项研究的目的是测试在手术部位的炎症和伤害性的生化介质的收集和测量的可行性，评估伤口和血清水平之间的关系，并确定介质的释放，疼痛和镇痛消费后剖宫产之间的任何关联交付。二十经历选修剖宫产脊髓麻醉的健康妇女参加。 1，6，24，和48小时后剖宫产伤口渗出液和血清介质，疼痛评分和止痛剂的消费。在创面渗液，调解员19 20出可靠的检测，包括IL -1β，IL - 2，IL - 4，IL - 6，IL - 7，IL - 8，IL - 10，IL - 12，IL - 13，白细胞介素体育伤口PG - E2和各种细胞因子-17，肿瘤坏死因子，INFγ，G - CSF，GM - CSF，MCP - 1和MIP -1β，神经生长因子（NGF），前列腺素E2（PG - E2的）和物质见顶早，而神经生长因子表现出更多的延迟​​释放。有伤口和血清细胞因子的浓度随时间变化的轮廓之间没有相关性。这项研究表明，在手术伤口的痛觉和炎症介质，在特定时间点的收集和测量的可行性。伤口和血清之间缺乏显着相关性，强调本地化的病状，如果要研究确定站点的具体发布的重要性。

Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

的技术来收集和测量在特定时间点的手术伤口的生化调解人。

在手术伤口的痛觉及炎症介质的收集和测量

We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. ...

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Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds 

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