Name: collecting and measuring nociceptive and inflammatory mediators in surgical wounds
Uploaded: 2008-10-29T00:00:00
Description: Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

박사 카발로의 작품은 여성의 건강과 아동 건강 및 보건 국립 연구소 (5K12 HD043452)의 인간 개발 국립 연구소에 대한 연구의 사무실에서 여성 건강 연구 부여에있는 한 건물 학제간 채용에 의해 지원됩니다. 드르 불안감은 대한 공급 (온 - Q ® PainBuster ® 수술 통증 시스템)와 자금을받은 I - 흐름에서 (레이크 포레스트, CA) 생화학 assays를 수행.

Nociceptive 및 염증성 생화 학적 매개체 모음 <ol><li> 온 - Q ® PainBuster ® 통증 시스템은 폐쇄 상처에 직전 수술 팀에 의해 피하 층에 삽입됩니다. 이 시스템은 지속적으로 subcutaneously 2 ML / H.의 속도로 상처에 정상적인 생리 (또는 국소 마취제) 제공 </li><li> 세 방향 꼭지는 지정된 시간 지점에서 상처 exudate의 뜻을 허용하려면이 시스템에 통합됩니다. </li><li> 프로토콜 (예, 1, 6, 24, 및 cesarean 배달 후 48 ?...

<ol>
	<li>Elshal, M. F., McCoy, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assays:+performance+evaluation+and+comparison+of+sensitivity+to+ELISA.">Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA.</a> Methods. 38, 317-323 (2006).</li><li>Heijmans-Antonissen, C., Wesseldijk, F., Munnikes, R. J., Huygen, F. J., van der Meijden, P., Hop, W. C., Hooijkaas, H., Zijlstra, F. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assay+for+detection+of+25+soluble+cytokines+in+blister+fluid+of+patients+with+complex+regional+pain+syndrome+type+1.">Multiplex bead array assay for detection of 25 soluble cytokines in blister fluid of patients with complex regional pain syndrome type 1.</a> Mediators Inflamm.. 28398, 1-8 (2006).</li><li>Buvanendran, A., Kroin, J. S., Berger, R. A., Hallab, N. J., Saha, C., Negrescu, C., Moric, M., Caicedo, M. S., Tuman, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Upregulation+of+prostaglandin+E2+and+interleukins+in+the+central+nervous+system+and+peripheral+tissue+during+and+after+surgery+in+humans.+.">Upregulation of prostaglandin E2 and interleukins in the central nervous system and peripheral tissue during and after surgery in humans. .</a> Anesthesiology. 104, 403-410 (2000).</li><li>Holzheimer, R. G., Steinmetz, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+and+systemic+concentrations+of+pro-+and+anti-inflammatory+cytokines+in+human+wounds.">Local and systemic concentrations of pro- and anti-inflammatory cytokines in human wounds.</a> Eur J Med Res. 5, 347-355 (2000).</li></ol>

온 - Q ® PainBuster ® 통증 시스템은 폐쇄 상처에 직전 피하 층의 전체 절개를 통해 삽입한다. 이것은 지정된 시간 간격으로 세 방향 꼭지를 통해 뜻을 용이하게합니다. 온 - Q ® 시스템은 지속적으로 2 ML / H.의 속도로 상처에 subcutaneously 정상적인 생리를 제공합니다 이것은 카테 테르가 응고 방지 및 exudate 샘플을 생산하는 시스템의 안정성을 향상시킵니다. exudate의 열망이 (가지 경우 약 5 %) 어려운 경우, (예를 들어,...

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded.
A fifth reference:
 5. Carvalho, B., Clark, D. J. &amp; Angst, M. S. Local and Systemic Release of Cytokines, Nerve Growth Factor, Prostaglandin E2, and Substance P in Incisional Wounds and Serum Following Cesarean Delivery. The Journal of Pain : official journal of the American Pain Society 9 (7), 650-657 (2008).
 was added. The abstract was updated to :
We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. Collecting site-specific biochemical markers allows us to evaluate the relationship between surgical wound and serum levels; determine any associations between mediator release, pain and analgesic consumption; and evaluate the effect of systemic and peripheral drug administration on surgical wound biochemistry.
This methodology has been applied to healthy women undergoing elective cesarean delivery with spinal anesthesia. Wound exudate and serum mediators, in conjunction with pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean delivery. Biochemical mediators that were detected included IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. We found no correlations between wound and serum cytokines concentrations or time-release profiles (J Pain. 2008 Jul 9(7):650-7). This article describes and demonstrates the feasibility of collecting and assaying nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between serum and wound levels shows the importance of determining site-specific release if surgical wounds and localized pathologies are to be studied.
from
The objectives of this study were to test the feasibility of collecting and measuring inflammatory and nociceptive biochemical mediators at the surgical site; to evaluate the relationship between wound and serum levels; and to determine any associations between mediator release, pain and analgesic consumption post-cesarean delivery. Twenty healthy women undergoing elective cesarean delivery with spinal anesthesia were enrolled. Wound exudate and serum mediators, pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean. In wound exudate, 19 out of 20 mediators were reliably detected including IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. Wound PG-E2 and various cytokines peaked early, whereas NGF showed a more delayed release. There were no correlations between the concentration versus time profile of wound and serum cytokines. This study demonstrates the feasibility of collecting and measuring nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between wound and serum levels emphasizes the importance of determining site-specific release if localized pathologies are to be studied.

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded. A revised abstract and the inclusion of a reference for figure 1 is republished here.

Erratum: Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

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<td>On-Q&reg; PainBuster&reg; Post-Op Pain Relief System </td>
<td>&nbsp;</td>
<td>I-Flow, Lake Forest, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Complete proteinase inhibitor </td>
<td>&nbsp;</td>
<td>Roche Bioscience, Palo Alto, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>17-multiplex bead immunoassay Bio-PlexTM plate</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Bio-Plex amine coupling kit</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>The NGF antibody DY256</td>
<td>&nbsp;</td>
<td>R&D Systems, Minneapolis, MN</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Prostaglandin E2 and substance P ELISA Kits </td>
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collecting and measuring nociceptive and inflammatory mediators in surgical wounds 

본 연구의 목적은 외과 사이트에서 염증 및 nociceptive 생화 학적 중재자를 수집 및 측정의 타당성을 테스트했고, 상처와 혈청 수준 사이의 관계를 평가 및 중재자 릴리스, 통증과 진통 소비 후 cesarean 사이의 연관 관계를 결정하는 배달. 척추 마취로 선택 cesarean 전달을받은 스물​​ 건강한 여성 등록되었습니다. 상처 exudate 및 혈청 중재자, 통증 점수와 진통제 소비는 1, 6, 24, 그리고 48시간 후 cesarean에서 측정되었다. 상처 exudate에서 20 중재자 19 밖은 안정적으로 IL - 1β, IL - 2, IL - 4, IL - 6, IL - 7, IL - 8, IL - 10, IL - 12, IL - 13, IL을 포함 발견되었습니다 -17, TNFα, INFγ, G - CSF, GM - CSF, MCP - 1, MIP - 1β, 신경 성장 인자 (NGF), 프로 스타 글란딘 E2 (PG - E2)와 물질 P. 상처 PG - E2와 다양한 크린 시토킨 일찍 만족 , NGF보다 지연 릴리스를 보여주 반면. 농도 비교 상처와 혈청 크린 시토킨 시간 프로파일 사이 상관 관계가 없었다. 이 연구는 특정 시간 지점에서 수술 상처에 nociceptive 및 염증 중재자를 수집 및 측정의 가능성을 보여줍니다. 상처와 혈청 수준 사이에 상당한 상관 관계의 부족화된 pathologies가 연구해야하는 경우 특정 사이트 릴리스 결정의 중요성을 강조하고 있습니다.

Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

특정 시간 지점에서 수술 상처를 생화 학적 중재자를 수집하고 측정하는 기술.

외과 듀엣으로 Nociceptive 및 염증성 중재자를 수집 및 측정

We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. ...

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Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds 

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