Name: collecting and measuring nociceptive and inflammatory mediators in surgical wounds
Uploaded: 2008-10-29T00:00:00
Description: Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

El trabajo del Dr. Carvalho se apoya en un edificio Carrera Interdisciplinaria de conceder Salud de la Mujer de investigación de la Oficina de Investigación en Salud de la Mujer y el Instituto Nacional de Salud Infantil y Desarrollo Humano de los Institutos Nacionales de Salud (5K12 HD043452). Angustia Dr. recibieron suministros (el on-Q ® PainBuster ® Post-Op Sistema de Alivio del Dolor) y la financiación para la realización de los ensayos bioquímicos de I-Flow (Lake Forest, CA).

Colección bioquímicos mediador inflamatorio y nociceptivo <ol><li> El On-Q ® PainBuster ® sistema de alivio del dolor se inserta en la capa subcutánea por el equipo quirúrgico justo antes de cierre de la herida. El sistema proporciona continuamente una solución salina normal (o anestesia local) por vía subcutánea en la herida a una velocidad de 2 ml / h. </li><li> Una llave de tres vías se incorpora a este sistema para permitir la aspiración de exudado de la herida en los puntos de tiempo especificado. </li>...

<ol>
	<li>Elshal, M. F., McCoy, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assays:+performance+evaluation+and+comparison+of+sensitivity+to+ELISA.">Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA.</a> Methods. 38, 317-323 (2006).</li><li>Heijmans-Antonissen, C., Wesseldijk, F., Munnikes, R. J., Huygen, F. J., van der Meijden, P., Hop, W. C., Hooijkaas, H., Zijlstra, F. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+bead+array+assay+for+detection+of+25+soluble+cytokines+in+blister+fluid+of+patients+with+complex+regional+pain+syndrome+type+1.">Multiplex bead array assay for detection of 25 soluble cytokines in blister fluid of patients with complex regional pain syndrome type 1.</a> Mediators Inflamm.. 28398, 1-8 (2006).</li><li>Buvanendran, A., Kroin, J. S., Berger, R. A., Hallab, N. J., Saha, C., Negrescu, C., Moric, M., Caicedo, M. S., Tuman, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Upregulation+of+prostaglandin+E2+and+interleukins+in+the+central+nervous+system+and+peripheral+tissue+during+and+after+surgery+in+humans.+.">Upregulation of prostaglandin E2 and interleukins in the central nervous system and peripheral tissue during and after surgery in humans. .</a> Anesthesiology. 104, 403-410 (2000).</li><li>Holzheimer, R. G., Steinmetz, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+and+systemic+concentrations+of+pro-+and+anti-inflammatory+cytokines+in+human+wounds.">Local and systemic concentrations of pro- and anti-inflammatory cytokines in human wounds.</a> Eur J Med Res. 5, 347-355 (2000).</li></ol>

El On-Q ® PainBuster Dolor ® Sistema de seguridad deben ser insertados a través de toda la incisión en la capa subcutánea justo antes de cierre de la herida. Esto facilita la aspiración a través de la llave de tres vías en los intervalos de tiempo especificados. El On-Q ® sistema de forma continua proporciona una solución salina normal por vía subcutánea en la herida a una velocidad de 2 ml / h. Esto evita que el catéter de la coagulación y mejora la fiabilidad del sistema para producir muestras de exudado. Si la aspiración d...

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded.
A fifth reference:
 5. Carvalho, B., Clark, D. J. &amp; Angst, M. S. Local and Systemic Release of Cytokines, Nerve Growth Factor, Prostaglandin E2, and Substance P in Incisional Wounds and Serum Following Cesarean Delivery. The Journal of Pain : official journal of the American Pain Society 9 (7), 650-657 (2008).
 was added. The abstract was updated to :
We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. Collecting site-specific biochemical markers allows us to evaluate the relationship between surgical wound and serum levels; determine any associations between mediator release, pain and analgesic consumption; and evaluate the effect of systemic and peripheral drug administration on surgical wound biochemistry.
This methodology has been applied to healthy women undergoing elective cesarean delivery with spinal anesthesia. Wound exudate and serum mediators, in conjunction with pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean delivery. Biochemical mediators that were detected included IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. We found no correlations between wound and serum cytokines concentrations or time-release profiles (J Pain. 2008 Jul 9(7):650-7). This article describes and demonstrates the feasibility of collecting and assaying nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between serum and wound levels shows the importance of determining site-specific release if surgical wounds and localized pathologies are to be studied.
from
The objectives of this study were to test the feasibility of collecting and measuring inflammatory and nociceptive biochemical mediators at the surgical site; to evaluate the relationship between wound and serum levels; and to determine any associations between mediator release, pain and analgesic consumption post-cesarean delivery. Twenty healthy women undergoing elective cesarean delivery with spinal anesthesia were enrolled. Wound exudate and serum mediators, pain scores and analgesics consumption were measured at 1, 6, 24, and 48 hours post-cesarean. In wound exudate, 19 out of 20 mediators were reliably detected including IL-1&beta;, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, TNF&alpha;, INF&gamma;, G-CSF, GM-CSF, MCP-1 and MIP-1&beta;, nerve growth factor (NGF), prostaglandin E2 (PG-E2) and substance P. Wound PG-E2 and various cytokines peaked early, whereas NGF showed a more delayed release. There were no correlations between the concentration versus time profile of wound and serum cytokines. This study demonstrates the feasibility of collecting and measuring nociceptive and inflammatory mediators in surgical wounds at specific time points. The lack of significant correlations between wound and serum levels emphasizes the importance of determining site-specific release if localized pathologies are to be studied.

A correction was made to: <a href="http://www.jove.com/video/962/">Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds</a>. A key reference was excluded. A revised abstract and the inclusion of a reference for figure 1 is republished here.

Erratum: Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

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<td>On-Q&reg; PainBuster&reg; Post-Op Pain Relief System </td>
<td>&nbsp;</td>
<td>I-Flow, Lake Forest, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Complete proteinase inhibitor </td>
<td>&nbsp;</td>
<td>Roche Bioscience, Palo Alto, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>17-multiplex bead immunoassay Bio-PlexTM plate</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Bio-Plex amine coupling kit</td>
<td>&nbsp;</td>
<td>BioRad, Hercules, CA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>The NGF antibody DY256</td>
<td>&nbsp;</td>
<td>R&D Systems, Minneapolis, MN</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Prostaglandin E2 and substance P ELISA Kits </td>
<td>&nbsp;</td>
<td>Assay Designs, Inc., Ann Arbor, Michigan</td>
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collecting and measuring nociceptive and inflammatory mediators in surgical wounds 

Los objetivos de este estudio fue probar la viabilidad de la recogida y medición de mediadores bioquímicos inflamatorios y nociceptivos en el sitio quirúrgico, para evaluar la relación entre la herida y los niveles séricos, y determinar las asociaciones entre la liberación de mediadores, el dolor y el consumo de analgésicos post-cesárea entrega. Veinte mujeres sanas sometidas a cesárea electiva con anestesia espinal se inscribieron. Exudado de la herida y los mediadores de suero, las puntuaciones de dolor y el consumo de analgésicos se mide a 1, 6, 24 y 48 horas post-cesárea. En el exudado de la herida, 19 de los 20 mediadores se detectaban correctamente incluyendo IL-1β, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL -17, TNFa, INFγ, G-CSF, GM-CSF, MCP-1 y MIP-1β, factor de crecimiento nervioso (NGF), la prostaglandina E2 (PG-E2) y la sustancia P. heridas PG-E2 y diversas citocinas alcanzó su punto máximo a principios , mientras que el NGF mostró una liberación más retardada. No hubo correlación entre la concentración frente al perfil temporal de la herida y citocinas séricas. Este estudio demuestra la viabilidad de la recogida y medición de mediadores inflamatorios y nociceptivos en las heridas quirúrgicas en momentos específicos. La falta de correlaciones significativas entre los niveles séricos de la herida y hace hincapié en la importancia de determinar el sitio específico de patologías localizadas en libertad si se van a estudiar.

Read this Scientific Article Protocol about Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds at JoVE.com

Collecting and Measuring Nociceptive and Inflammatory Mediators in Surgical Wounds

Una técnica para recoger y medir los mediadores bioquímicos de la herida quirúrgica en momentos específicos.

La recogida y medición de mediadores inflamatorios y nociceptivos en las heridas quirúrgicas

We describe a methodology by which we are able to collect and measure inflammatory and nociceptive biochemical mediators at the surgical wound site. ...

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