Chong Zhang

Rapid detection of a gfp-marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery.

A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes.

Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process.

Temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase.

Correcting for the inner filter effect in measurements of fluorescent proteins in high-cell-density cultures.

Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production.

Alteration of hydrogen metabolism of ldh-deleted Enterobacter aerogenes by overexpression of NAD+-dependent formate dehydrogenase.

[Heterologous expression of particulate methane monooxygenase in different host cells].

Reconstruction of the violacein biosynthetic pathway from Duganella sp. B2 in different heterologous hosts.

[Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants].

Bioengineering of the Enterobacter aerogenes strain for biohydrogen production.

Luciferase and fluorescent protein as dual reporters analyzing the effect of n-dodecyltrimethylammonium bromide on the physiology of Pseudomonas putida.

Improved hydrogen production under microaerophilic conditions by overexpression of polyphosphate kinase in Enterobacter aerogenes.

Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli.

Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum.

[Recent progress in fusion enzyme design and applications].

Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection.

Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols.

Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility.

Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.

Controllable production of low molecular weight heparins by combinations of heparinase I/II/III.

Rapid mutation of Spirulina platensis by a new mutagenesis system of atmospheric and room temperature plasmas (ARTP) and generation of a mutant library with diverse phenotypes.

Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool.

Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community.

High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway.

Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production.

DomSign: a top-down annotation pipeline to enlarge enzyme space in the protein universe.

Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis.

Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes.

Construction of a linker library with widely controllable flexibility for fusion protein design.

Insights into the global regulation of anaerobic metabolism for improved biohydrogen production.

Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli.

Bioconversion of methanol to value-added mevalonate by engineered Methylobacterium extorquens AM1 containing an optimized mevalonate pathway.

Characterization of a novel Acinetobacter baumannii xanthine dehydrogenase expressed in Escherichia coli.

A study on the effects of linker flexibility on acid phosphatase PhoC-GFP fusion protein using a novel linker library.

Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin.

Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria.

Xanthine dehydrogenase: An old enzyme with new knowledge and prospects.

Improved production of trans-4-hydroxy-l-proline by chromosomal integration of the Vitreoscilla hemoglobin gene into recombinant Escherichia coli with expression of proline-4-hydroxylase.

Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors.

Biosensor-assisted transcriptional regulator engineering for Methylobacterium extorquens AM1 to improve mevalonate synthesis by increasing the acetyl-CoA supply.

Non-anticoagulant effects of low molecular weight heparins in inflammatory disorders: A review.

Erratum to: Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors.

Metabolic engineering of Escherichia coli cell factory for highly active xanthine dehydrogenase production.

Discovery of enzymatically depolymerized heparins capable of treating Bleomycin-induced pulmonary injury and fibrosis in mice.

Maltose Utilization as a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production.

Effects of Enzymatically Depolymerized Low Molecular Weight Heparins on CCl-Induced Liver Fibrosis.

Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up.

A rapid and specific colorimetric method for free tryptophan quantification.

Targeted mutagenesis: A sniper-like diversity generator in microbial engineering.

Cre/loxP-Mediated Multicopy Integration of the Mevalonate Operon into the Genome of Methylobacterium extorquens AM1.

Design of Fusion Proteins for Efficient and Soluble Production of Immunogenic Ebola Virus Glycoprotein in Escherichia coli.

MiYA, an efficient machine-learning workflow in conjunction with the YeastFab assembly strategy for combinatorial optimization of heterologous metabolic pathways in Saccharomyces cerevisiae.

Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution.

Therapeutic experience of severe and recurrent secondary hyperparathyroidism in a patient on hemodialysis for 18 years: A case report.

Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES.

Enhanced Production of Crude Violacein from Glucose in Escherichia coli by Overexpression of Rate-Limiting Key Enzyme(S) Involved in Violacein Biosynthesis.

Structural characterization and in vitro antioxidant activities of chondroitin sulfate purified from Andrias davidianus cartilage.

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.

Improved sgRNA design in bacteria via genome-wide activity profiling.

Current Status of Surgical Treatment of Biliary Diseases in Elderly Patients in China.

Genome-wide screening identifies promiscuous phosphatases impairing terpenoid biosynthesis in Escherichia coli.

E-cadherin is Required for the Homeostasis of Lgr5 Gastric Antral Stem Cells.

Mutagenesis of Rhodobacter sphaeroides using atmospheric and room temperature plasma treatment for efficient production of coenzyme Q10.

The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication.

In vivo continuous evolution of metabolic pathways for chemical production.

Gel microdroplet-based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris.

[Micro-cultivation system in microbiology: frontiers and prospects].

[Interaction between orally administrated heparin and intestinal microbiota in mice].

Dynamics of transcription-translation coordination tune bacterial indole signaling.

Highly Efficient Capture of Marine Microbial Strains in Seawater Using Bare FeO Magnetic Beads.

Microbial microdroplet culture system (MMC): An integrated platform for automated, high-throughput microbial cultivation and adaptive evolution.

Establishment of CRISPR interference in Methylorubrum extorquens and application of rapidly mining a new phytoene desaturase involved in carotenoid biosynthesis.

Hybrid nanoparticles with CO-responsive shells and fluorescence-labelled magnetic cores.

Electrochemically-responsive magnetic nanoparticles for reversible protein adsorption.

Increased stability and intracellular antioxidant activity of chlorogenic acid depend on its molecular interaction with wheat gluten hydrolysate.

Empowering a Methanol-Dependent via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System.

A heparin derivatives library constructed by chemical modification and enzymatic depolymerization for exploitation of non-anticoagulant functions.

Design and construction of chimeric linker library with controllable flexibilities for precision protein engineering.

Ionic covalent organic frameworks for the magnetic solid-phase extraction of perfluorinated compounds in environmental water samples.

Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens.

Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells.

Effects of bioactive components of Pu-erh tea on gut microbiomes and health: A review.

[Technology development and instrumentation of a high-throughput and automated microbial microdroplet culture system for microbial evolution and screening].

Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics.

Genome-wide genotype-phenotype associations in microbes.

Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution.

[Functional discovery and production technology for natural bioactive peptides].

Construction and characterization of novel bifunctional fusion proteins composed of alcohol dehydrogenase and NADH oxidase with efficient oxidized cofactor regeneration.

New Method for Genome-Scale Functional Genomic Study in Bacteria with Superior Performance: CRISPR Interference Screen.

A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris.

Composite SPE Paper Membrane Based on the Functional Superstructure of Metal-Organic Frameworks and Ionic Liquids for Detection of Tetracycline-like Antibiotics.

Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast.

Development of a novel platform for recombinant protein production in on ethanol.

Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts.

Modeling the modulation characteristics of the Bradbury-Nielsen gate in ion mobility spectrometers.

The root-specific NtR12 promoter-based expression of RIP increased the resistance against bacterial wilt disease in tobacco.

Sodium formate redirects carbon flux and enhances heterologous mevalonate production in Methylobacterium extorquens AM1.

Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively.

Cross-species binding spectrum of ACE2 to SARS-CoV-2 RBD.

Single-cell microliter-droplet screening system (MISS Cell): An integrated platform for automated high-throughput microbial monoclonal cultivation and picking.

CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion.

CRISPR-Cas12a-assisted elimination of the non-specific signal from non-specific amplification in the Exponential Amplification Reaction.

Recent advances of integrated microfluidic suspension cell culture system.

Hotspots and difficulties of biliary surgery in older patients.

Mining and Validation of Novel Hemp Seed-Derived DPP-IV-Inhibiting Peptides Using a Combination of Multi-omics and Molecular Docking.

Exploring the Trans-Cleavage Activity with Rolling Circle Amplification for Fast Detection of miRNA.

Exploration of DPP-IV Inhibitory Peptide Design Rules Assisted by the Deep Learning Pipeline That Identifies the Restriction Enzyme Cutting Site.

Deep-learning-assisted Sort-Seq enables high-throughput profiling of gene expression characteristics with high precision.

Enhancing ion mobility spectrometry performance through a programmable ion swarm shaping method based on Bradbury-Nielsen gates.

Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes.

Deep whole-genome analysis of 494 hepatocellular carcinomas.

Encoding Genetic Circuits with DNA Barcodes Paves the Way for High-Throughput Profiling of Dose-Response Curves of Metabolite Biosensors.

Strain engineering of Bacillus coagulans with high osmotic pressure tolerance for effective L-lactic acid production from sweet sorghum juice under unsterile conditions.

Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis.

Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting .

Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth.

Characterization of maltose-binding protein-fused heparinases with enhanced thermostability by application of rigid and flexible linkers.

Effect of ionic wind on the sample distribution in corona discharge ion mobility spectrometry.

GLiDe: a web-based genome-scale CRISPRi sgRNA design tool for prokaryotes.