Department of Chemical Engineering,
Institute of Biochemical Engineering,
Key Laboratory of Industrial Biocatalysis,
Ministry of Education,
Center for Synthetic & Systems Biology,
Department of Chemical Engineering, Institute of Biochemical Engineering,
Key Laboratory of Industrial Biocatalysis, Ministry of Education
Chong Zhang is an Associate Professor in the Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University in Beijing, China. He received his Bachelor's degree and a Ph.D. from the Tsinghua University, China.
During Dr. Zhang’s training (2002-2008) he focused on the study of fluorescence-based quantification of Enterobacter aerogenes and its NADH pathway mechanism for hydrogen production. As a post-doctoral fellow (2008 to 2010) in the Xin-hui Xing’s lab at the Key Laboratory of Industrial Biocatalysis, Ministry of Education in Tsinghua University, China, he further studied the fluorescent proteins as a visible molecular signal for rapid quantification of bioprocesses, such as correcting for the inner filter effect in measurements of fluorescent proteins in high-cell-density cultures and the temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase.
Dr. Zhang was recruited as a research assistant in 2010 and then became an associate professor in 2011 at Tsinghua University. He received many awards, such as the Young Asian Biotechnologist Prize (2020), the First Prize of Technology Invention Award of China National Light Industry (2019), the “Lun Shiyi” Outstanding Young Scholar Award in the Fermentation Engineering (2018) and the gold medal at 45th International Exhibition of Inventions of Geneva (2017). His research programs are committed to microbial intelligent manufacturing, study and development of original high-throughput genotype-phenotype association (GPA) technologies and equipment, including high-throughput characterization and continuous evolution of microbial industrial phenotypes, in-depth mining of genome-scale genotype function, research equipment of genotype and industrial phenotype association.
Rapid detection of a gfp-marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery.
FEMS microbiology letters Aug, 2005 | Pubmed ID: 16006057
A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes.
Biotechnology letters Jul, 2005 | Pubmed ID: 16132849
Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process.
Bioresource technology Jun, 2009 | Pubmed ID: 19231169
Temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase.
Applied microbiology and biotechnology Sep, 2009 | Pubmed ID: 19390851
Correcting for the inner filter effect in measurements of fluorescent proteins in high-cell-density cultures.
Analytical biochemistry Jul, 2009 | Pubmed ID: 19394302
Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production.
Biotechnology letters Oct, 2009 | Pubmed ID: 19533026
Alteration of hydrogen metabolism of ldh-deleted Enterobacter aerogenes by overexpression of NAD+-dependent formate dehydrogenase.
Applied microbiology and biotechnology Mar, 2010 | Pubmed ID: 19830418
[Heterologous expression of particulate methane monooxygenase in different host cells].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Aug, 2009 | Pubmed ID: 19938451
Reconstruction of the violacein biosynthetic pathway from Duganella sp. B2 in different heterologous hosts.
Applied microbiology and biotechnology Apr, 2010 | Pubmed ID: 20012278
[Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Mar, 2011 | Pubmed ID: 21650028
Bioengineering of the Enterobacter aerogenes strain for biohydrogen production.
Bioresource technology Sep, 2011 | Pubmed ID: 21764301
Luciferase and fluorescent protein as dual reporters analyzing the effect of n-dodecyltrimethylammonium bromide on the physiology of Pseudomonas putida.
Applied microbiology and biotechnology Jan, 2012 | Pubmed ID: 22075632
Improved hydrogen production under microaerophilic conditions by overexpression of polyphosphate kinase in Enterobacter aerogenes.
Enzyme and microbial technology Feb, 2011 | Pubmed ID: 22112830
Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli.
Applied microbiology and biotechnology May, 2012 | Pubmed ID: 22588503
Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum.
Applied biochemistry and biotechnology Jun, 2012 | Pubmed ID: 22592776
[Recent progress in fusion enzyme design and applications].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Apr, 2012 | Pubmed ID: 22803390
Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection.
Journal of biotechnology Jan, 2013 | Pubmed ID: 23073152
Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols.
Applied and environmental microbiology Apr, 2013 | Pubmed ID: 23354700
Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility.
Microbial cell factories Mar, 2013 | Pubmed ID: 23497261
Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.
Microbes and environments , 2013 | Pubmed ID: 24240187
Controllable production of low molecular weight heparins by combinations of heparinase I/II/III.
Carbohydrate polymers Jan, 2014 | Pubmed ID: 24299802
Rapid mutation of Spirulina platensis by a new mutagenesis system of atmospheric and room temperature plasmas (ARTP) and generation of a mutant library with diverse phenotypes.
PloS one , 2013 | Pubmed ID: 24319517
Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool.
Applied microbiology and biotechnology Jun, 2014 | Pubmed ID: 24769904
Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community.
Bioresource technology Jan, 2015 | Pubmed ID: 25459816
High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway.
Microbial cell factories Jan, 2015 | Pubmed ID: 25592762
Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production.
Journal of bioscience and bioengineering Oct, 2015 | Pubmed ID: 25795571
DomSign: a top-down annotation pipeline to enlarge enzyme space in the protein universe.
BMC bioinformatics Mar, 2015 | Pubmed ID: 25888481
Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis.
Applied microbiology and biotechnology Jul, 2015 | Pubmed ID: 26025015
Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes.
Bioresource technology Oct, 2015 | Pubmed ID: 26188552
Construction of a linker library with widely controllable flexibility for fusion protein design.
Applied microbiology and biotechnology Jan, 2016 | Pubmed ID: 26394862
Insights into the global regulation of anaerobic metabolism for improved biohydrogen production.
Bioresource technology Jan, 2016 | Pubmed ID: 26476162
Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli.
Metabolic engineering Jan, 2016 | Pubmed ID: 26506462
Bioconversion of methanol to value-added mevalonate by engineered Methylobacterium extorquens AM1 containing an optimized mevalonate pathway.
Applied microbiology and biotechnology Mar, 2016 | Pubmed ID: 26521242
Characterization of a novel Acinetobacter baumannii xanthine dehydrogenase expressed in Escherichia coli.
Biotechnology letters Feb, 2016 | Pubmed ID: 26543035
A study on the effects of linker flexibility on acid phosphatase PhoC-GFP fusion protein using a novel linker library.
Enzyme and microbial technology Feb, 2016 | Pubmed ID: 26777244
Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin.
Carbohydrate polymers Jun, 2016 | Pubmed ID: 27083825
Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria.
Journal of bioscience and bioengineering Oct, 2016 | Pubmed ID: 27150511
Xanthine dehydrogenase: An old enzyme with new knowledge and prospects.
Bioengineered Nov, 2016 | Pubmed ID: 27537049
Improved production of trans-4-hydroxy-l-proline by chromosomal integration of the Vitreoscilla hemoglobin gene into recombinant Escherichia coli with expression of proline-4-hydroxylase.
Journal of bioscience and bioengineering Jan, 2017 | Pubmed ID: 27567047
Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors.
Biotechnology for biofuels , 2016 | Pubmed ID: 27895708
Biosensor-assisted transcriptional regulator engineering for Methylobacterium extorquens AM1 to improve mevalonate synthesis by increasing the acetyl-CoA supply.
Metabolic engineering 01, 2017 | Pubmed ID: 27919791
Non-anticoagulant effects of low molecular weight heparins in inflammatory disorders: A review.
Carbohydrate polymers Mar, 2017 | Pubmed ID: 28115102
Erratum to: Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors.
Biotechnology for biofuels , 2017 | Pubmed ID: 28174602
Metabolic engineering of Escherichia coli cell factory for highly active xanthine dehydrogenase production.
Bioresource technology Dec, 2017 | Pubmed ID: 28610971
Discovery of enzymatically depolymerized heparins capable of treating Bleomycin-induced pulmonary injury and fibrosis in mice.
Carbohydrate polymers Oct, 2017 | Pubmed ID: 28821136
Maltose Utilization as a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production.
ACS synthetic biology 12, 2017 | Pubmed ID: 28841296
Effects of Enzymatically Depolymerized Low Molecular Weight Heparins on CCl-Induced Liver Fibrosis.
Frontiers in pharmacology , 2017 | Pubmed ID: 28871223
Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up.
Current opinion in biotechnology 04, 2018 | Pubmed ID: 28892667
A rapid and specific colorimetric method for free tryptophan quantification.
Talanta Jan, 2018 | Pubmed ID: 28917797
Targeted mutagenesis: A sniper-like diversity generator in microbial engineering.
Synthetic and systems biotechnology Jun, 2017 | Pubmed ID: 29062964
Cre/loxP-Mediated Multicopy Integration of the Mevalonate Operon into the Genome of Methylobacterium extorquens AM1.
Applied biochemistry and biotechnology Jul, 2018 | Pubmed ID: 29243041
Design of Fusion Proteins for Efficient and Soluble Production of Immunogenic Ebola Virus Glycoprotein in Escherichia coli.
Biotechnology journal Jun, 2018 | Pubmed ID: 29500882
MiYA, an efficient machine-learning workflow in conjunction with the YeastFab assembly strategy for combinatorial optimization of heterologous metabolic pathways in Saccharomyces cerevisiae.
Metabolic engineering 05, 2018 | Pubmed ID: 29627507
Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution.
Biotechnology journal Jun, 2018 | Pubmed ID: 29729127
Therapeutic experience of severe and recurrent secondary hyperparathyroidism in a patient on hemodialysis for 18 years: A case report.
Medicine May, 2018 | Pubmed ID: 29768384
Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES.
Nature communications 05, 2018 | Pubmed ID: 29789541
Enhanced Production of Crude Violacein from Glucose in Escherichia coli by Overexpression of Rate-Limiting Key Enzyme(S) Involved in Violacein Biosynthesis.
Applied biochemistry and biotechnology Dec, 2018 | Pubmed ID: 29797295
Structural characterization and in vitro antioxidant activities of chondroitin sulfate purified from Andrias davidianus cartilage.
Carbohydrate polymers Sep, 2018 | Pubmed ID: 29891311
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Nature communications 06, 2018 | Pubmed ID: 29946130
Improved sgRNA design in bacteria via genome-wide activity profiling.
Nucleic acids research 08, 2018 | Pubmed ID: 29982721
Current Status of Surgical Treatment of Biliary Diseases in Elderly Patients in China.
Chinese medical journal Aug, 2018 | Pubmed ID: 30058588
Genome-wide screening identifies promiscuous phosphatases impairing terpenoid biosynthesis in Escherichia coli.
Applied microbiology and biotechnology Nov, 2018 | Pubmed ID: 30229323
E-cadherin is Required for the Homeostasis of Lgr5 Gastric Antral Stem Cells.
International journal of biological sciences , 2019 | Pubmed ID: 30662345
Mutagenesis of Rhodobacter sphaeroides using atmospheric and room temperature plasma treatment for efficient production of coenzyme Q10.
Journal of bioscience and bioengineering Jun, 2019 | Pubmed ID: 30709705
The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication.
Nature genetics 05, 2019 | Pubmed ID: 31043757
In vivo continuous evolution of metabolic pathways for chemical production.
Microbial cell factories May, 2019 | Pubmed ID: 31088458
Gel microdroplet-based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris.
Journal of bioscience and bioengineering Dec, 2019 | Pubmed ID: 31235414
[Micro-cultivation system in microbiology: frontiers and prospects].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Jul, 2019 | Pubmed ID: 31328472
[Interaction between orally administrated heparin and intestinal microbiota in mice].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Sep, 2019 | Pubmed ID: 31559755
Dynamics of transcription-translation coordination tune bacterial indole signaling.
Nature chemical biology 04, 2020 | Pubmed ID: 31873224
Highly Efficient Capture of Marine Microbial Strains in Seawater Using Bare FeO Magnetic Beads.
Current microbiology Jul, 2020 | Pubmed ID: 32103330
Microbial microdroplet culture system (MMC): An integrated platform for automated, high-throughput microbial cultivation and adaptive evolution.
Biotechnology and bioengineering 06, 2020 | Pubmed ID: 32159223
Establishment of CRISPR interference in Methylorubrum extorquens and application of rapidly mining a new phytoene desaturase involved in carotenoid biosynthesis.
Applied microbiology and biotechnology May, 2020 | Pubmed ID: 32215707
Hybrid nanoparticles with CO-responsive shells and fluorescence-labelled magnetic cores.
Journal of materials chemistry. B Jan, 2014 | Pubmed ID: 32261388
Electrochemically-responsive magnetic nanoparticles for reversible protein adsorption.
Journal of materials chemistry. B Jun, 2016 | Pubmed ID: 32263099
Increased stability and intracellular antioxidant activity of chlorogenic acid depend on its molecular interaction with wheat gluten hydrolysate.
Food chemistry Apr, 2020 | Pubmed ID: 32387948
Empowering a Methanol-Dependent via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System.
Frontiers in bioengineering and biotechnology , 2020 | Pubmed ID: 32733857
A heparin derivatives library constructed by chemical modification and enzymatic depolymerization for exploitation of non-anticoagulant functions.
Carbohydrate polymers Dec, 2020 | Pubmed ID: 32933671
Design and construction of chimeric linker library with controllable flexibilities for precision protein engineering.
Methods in enzymology , 2021 | Pubmed ID: 33482990
Ionic covalent organic frameworks for the magnetic solid-phase extraction of perfluorinated compounds in environmental water samples.
Mikrochimica acta 01, 2021 | Pubmed ID: 33483792
Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens.
Metabolic engineering 03, 2021 | Pubmed ID: 33493644
Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells.
Nucleic acids research 02, 2021 | Pubmed ID: 33503261
Effects of bioactive components of Pu-erh tea on gut microbiomes and health: A review.
Food chemistry Aug, 2021 | Pubmed ID: 33743430
[Technology development and instrumentation of a high-throughput and automated microbial microdroplet culture system for microbial evolution and screening].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Mar, 2021 | Pubmed ID: 33783163
Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics.
Biosensors & bioelectronics Jul, 2021 | Pubmed ID: 33839534
Genome-wide genotype-phenotype associations in microbes.
Journal of bioscience and bioengineering Jul, 2021 | Pubmed ID: 33895083
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution.
Trends in biotechnology Jan, 2022 | Pubmed ID: 33958227
[Functional discovery and production technology for natural bioactive peptides].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Jun, 2021 | Pubmed ID: 34227301
Construction and characterization of novel bifunctional fusion proteins composed of alcohol dehydrogenase and NADH oxidase with efficient oxidized cofactor regeneration.
Biotechnology and applied biochemistry Jul, 2021 | Pubmed ID: 34269481
New Method for Genome-Scale Functional Genomic Study in Bacteria with Superior Performance: CRISPR Interference Screen.
Methods in molecular biology (Clifton, N.J.) , 2022 | Pubmed ID: 34709614
A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris.
Applied microbiology and biotechnology Dec, 2021 | Pubmed ID: 34773154
Composite SPE Paper Membrane Based on the Functional Superstructure of Metal-Organic Frameworks and Ionic Liquids for Detection of Tetracycline-like Antibiotics.
ACS applied materials & interfaces Jan, 2022 | Pubmed ID: 34968035
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast.
ACS synthetic biology Jan, 2022 | Pubmed ID: 35089702
Xingjin Jian*,1,2,
Xiaojie Guo*,1,2,3,
Jia Wang4,
Zheng Lin Tan1,2,6,
Xin-hui Xing1,2,5,
Liyan Wang3,
Chong Zhang1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University,
2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University,
3, Luoyang TMAXTREE Biotechnology Co., Ltd.,
4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University,
5Center for Synthetic & Systems Biology, Tsinghua University,
6School of Life Science and Technology, Tokyo Institute of Technology
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