Science Education - Advanced Biology
Immunology
17:07Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur ...
11:35Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes
Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur ...
14:22ELISA Assays: Indirect, Sandwich, and Competitive
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 ...
11:53ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes
Source: Tonya J. Webb1 1 Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and Stewart Greenebaum ...
13:24Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy
Source: Michael S. Lee1 and Tonya J. Webb1 1 Department of Microbiology and Immunology, University of Maryland School of Medicine and the Marlene and ...
13:21Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, ...
09:56Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections
Source: Thomas Chaffee1, Thomas S. Griffith2,3,4, and Kathryn L. Schwertfeger1,3,4 1 Department of Lab Medicine and Pathology, University of Minnesota, ...
13:13Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
Source: Dominique R. Bollino1, Eric A. Legenzov2, Tonya J. Webb1 1 Department of Microbiology and Immunology, University of Maryland School of Medicine ...
13:46Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads
Source: Susannah C. Shissler1, Tonya J. Webb1 1 Department of Microbiology and Immunology, University of Maryland, Baltimore, MD 21201 Immunoprecipitation ...
10:57Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphpoiesis, Department of Immunology, Pasteur ...
11:04Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur ...
11:48Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, ...